Emergence of XDR Escherichia coli carrying both blaNDM and mcr-1 genes in chickens at slaughter and the characterization of two novel blaNDM-bearing plasmids

Lv, Luchao; Zeng, Zhenling; Song, Qianhua; Cao, Yuping; Wang, Jing; Li, Wei; Wen, Qiaoling; Zhang, Qianhui; Wan, Miao; Yang, Jun; Liu, Jianhua

doi:10.1093/jac/dky176

Cited by 11 publications

(5 citation statements)

References 13 publications

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“…3 Though carbapenems are not legally prescribed for use in livestock production, the occurrence of carbapenemresistant Enterobacteriaceae (CRE), especially NDMproducing Enterobacteriaceae has been increasingly reported in livestock and meat products in the world. [7][8][9] Yet, systematic study on the prevalence and characterization of NDM-producing Enterobacteriaceae from food animals and animal-derived foods remain to be sporadic in Pakistan. 10 Here, for the first time, we characterized two NDM-producing Escherichia coli strains that were recovered from retail chicken meat samples in Pakistan.…”

Section: Introductionmentioning

confidence: 99%

Emergence of Almost Identical F36:A-:B32 Plasmids Carrying blaNDM-5 and qepA in Escherichia coli from Both Pakistan and Canada

Baloch¹,

Lv²,

Yi³

et al. 2019

IDR

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The New Delhi Metallo-β-lactamase (NDM) producing Enterobacteriaceae is spreading worldwide. Although the bla NDM gene has been identified in animal associated Enterobacteriaceae isolates in many countries, little is known about its occurrence in animal products in Pakistan. In this study, 13 Escherichia coli isolates were collected from chicken meat samples in Pakistan. Two isolates, 15978 and C4109, exhibited reduced susceptibility (MIC ≥1 μg/mL) to imipenem, and carried bla NDM-5 and bla NDM-7 gene, respectively. Whole-genome sequencing and Oxford Nanopore MinION sequencing revealed that 15978 and C4109 belonged to ST156 and ST167, respectively. bla NDM-7 was carried by an IncX3 plasmid that has disseminated worldwide, whereas bla NDM-5 was located on an F36: A-: B32 plasmid, which shared high identity with two plasmids carried by E. coli isolates from other countries (one from a patient in Canada). To the best of our knowledge, this is the first report characterizing bla NDM -carrying plasmids from chicken meat samples in Pakistan. The dissemination of almost identical bla NDM-5 -bearing F36:A-:B32 and bla NDM-7 -bearing IncX3 plasmids in different countries highlights the importance of international trade and travel in the spread of antimicrobial resistance strains and plasmids worldwide.

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Section: Introductionmentioning

confidence: 99%

Emergence of Almost Identical F36:A-:B32 Plasmids Carrying blaNDM-5 and qepA in Escherichia coli from Both Pakistan and Canada

Baloch¹,

Lv²,

Yi³

et al. 2019

IDR

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“…The VR2 comprised a typical bla NDM−1 unit (trpF-blebla NDM−1 ), a truncated catB, and an arr-3 gene. This VR2 structure flanked by ISCR1 and 3 CS has also been found in several enterobacterial plasmids including E. coli plasmids p1540-2 (CP019053.1), p6061604-KPC (MN823987.1), and pHNSD133T1 (MG196293.1) (Lv et al, 2018), Citrobacter freundii plasmid pCf75 (CP047308.1) (Li et al, 2020), and Klebsiella pneumoniae plasmid pNDM_3214 (CP028851.1) (Liu and Su, 2019). The MDR region (InSGI1-2NDM) of SGI1-2NDM also contained an In4-type complex class 1 integron.…”

Section: Genetic Features Of Two Novel Salmonella Genomic Island 1 Va...mentioning

confidence: 73%

Nosocomial Outbreak of Carbapenemase-Producing Proteus mirabilis With Two Novel Salmonella Genomic Island 1 Variants Carrying Different blaNDM–1 Gene Copies in China

Yang¹,

Chen

et al. 2022

Front. Microbiol.

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NDM-1-producing multidrug-resistant Proteus mirabilis brings formidable clinical challenges. We report a nosocomial outbreak of carbapenem-resistant P. mirabilis in China. Six P. mirabilis strains collected in the same ward showed close phylogenetic relatedness, indicating clonal expansion. Illumina and MinION sequencing revealed that three isolates harbored a novel Salmonella genomic island 1 carrying a blaNDM–1 gene (SGI1-1NDM), while three other isolates showed elevated carbapenem resistance and carried a similar SGI1 but with two blaNDM–1 gene copies (SGI1-2NDM). Four new single nucleotide mutations were present in the genomes of the two-blaNDM–1-harboring isolates, indicating later emergence of the SGI1-2NDM structure. Passage experiments indicated that both SGI variants were stably persistent in this clone without blaNDM–1 copy number changes. This study characterizes two novel blaNDM–1-harboring SGI1 variants in P. mirabilis and provides a new insight into resistance gene copy number variation in bacteria.

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“…Therefore, the presence of XDR E. coli, isolated from the influent of WWTP located in a city, along with the carbapenem-resistance gene raises public health concerns due to the possible dissemination of ARGs to other pathogenic bacteria, and difficulty in treatment of infections. Indeed, XDR pathogenic E. coli strains have been reported from human patients (harboring bla KPC−2 ) (Jeong et al, 2018) and from chickens (co-producing bla NDM and mcr-1) (Lv et al, 2018), increasing the likelihood of infectious disease outbreaks. The characteristics of XDR E. coli strain N7 can be attributed to the presence of corresponding resistance genes located on two plasmids of an IncX3 and a hybrid IncHI2/N.…”

Section: Discussionmentioning

confidence: 99%

Emergence of High Level Carbapenem and Extensively Drug Resistant Escherichia coli ST746 Producing NDM-5 in Influent of Wastewater Treatment Plant, Seoul, South Korea

et al. 2021

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High level carbapenem and extensively drug resistant (XDR) Escherichia coli strain N7, which produces a variant of New Delhi metallo-β-lactamase (NDM-5), was isolated from the influent of the Jungnang wastewater treatment plant located on Han River, Seoul, South Korea. Phenotypic and genotypic resistances to carbapenem were tested using agar and broth dilution methods, and polymerase chain reaction. Whole-genome sequencing was performed to characterize the genetic structure of strain N7. E. coli strain N7, which harbors the blaNDM–5 gene, showed high level of carbapenem resistance at concentrations of doripenem (512 mg/L) and meropenem (256 mg/L), and XDR to 15 antibiotics. Based on the genomic sequence analysis, two plasmids, a hybrid IncHI2/N-type and an IncX3 type, were present. The former contains a cluster (blaNDM–5-bleMBL-trpF-dsbD) bracketed by multi-insertional sequences, IS3000, ISAba125, IS5, and IS26. The latter carries the following resistance genes: blaCTX–14, aac(3)-IV, aadA1, aadA2, aph(3′)-Ia, aph(4)-Ia, sul1, sul2, sul3, dfrA12, fosA3, oqxA, oqxB, mph(A), and floR, and cmlA1. The chromosome, contig3, and contig5 also carry blaCTX–64 and mdf(A), tet(A), and erm(B), tet(M) and aadA22, respectively. Strain N7 also harbors virulence factors such as fimH, flu, ecpABCDE, sfmA, hlyE, and gadA. This study demonstrates the emergence of high level carbapenem resistant XDR E. coli strain N7 containing blaNDM–5 in aquatic environment, Seoul, South Korea. Due to the presence of mobile genetic elements, this strain could horizontally transfer resistance genes, including blaNDM–5 to environmental bacteria. Thus, it is necessary to conduct continuous surveillance for carbapenem resistance in various aquatic environments.

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Emergence of XDR Escherichia coli carrying both blaNDM and mcr-1 genes in chickens at slaughter and the characterization of two novel blaNDM-bearing plasmids

Cited by 11 publications

References 13 publications

<p>Emergence of Almost Identical F36:A-:B32 Plasmids Carrying <em>bla</em><sub>NDM-5</sub> and <em>qepA</em> in <em>Escherichia coli </em>from Both Pakistan and Canada</p>

<p>Emergence of Almost Identical F36:A-:B32 Plasmids Carrying <em>bla</em><sub>NDM-5</sub> and <em>qepA</em> in <em>Escherichia coli </em>from Both Pakistan and Canada</p>

Nosocomial Outbreak of Carbapenemase-Producing Proteus mirabilis With Two Novel Salmonella Genomic Island 1 Variants Carrying Different blaNDM–1 Gene Copies in China

Emergence of High Level Carbapenem and Extensively Drug Resistant Escherichia coli ST746 Producing NDM-5 in Influent of Wastewater Treatment Plant, Seoul, South Korea

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