Growth Factors Release From Concentrated Growth Factors: Effect of β-Tricalcium Phosphate Addition

Bonazza, Veronica; Hajistilly, Christopher; Patel, Dinker; Patel, J R; Woo, Rita; Cocchi, Marco Angelo; Buffoli, Barbara; Lancini, Davide; Gheno, Ezio; Rezzani, Rita; Jahanzeb, Sadia; Hunnisett, Adrian; Almasri, Maher; Lucchina, A Greco; Brucoli, Matteo; Mortellaro, Carmen; Rodella, Luigi Fabrizio

doi:10.1097/scs.0000000000004607

Cited by 26 publications

(22 citation statements)

References 50 publications

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“…In the present study, a slow release of BMP-2 was detected during at least 7 days in both L-PRF and CGF, even if the quantities remained quite small. It is interesting to notice that small quantities of BMP-2 has also been detected in L-PRF (Dohan Ehrenfest et al, 2018) and CGF (Bonazza et al, 2018) in previous publications. It has been reported that the different cell populations (mostly leukocytes) living in the fibrin clot may contribute to the release of BMP-2 (Dohan Ehrenfest et al, 2018).…”

Section: Discussionmentioning

confidence: 65%

Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study

Zhang

Zhao

et al. 2019

PeerJ

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BackgroundPlatelet concentrates have been used in tissue regeneration. The purpose of this study was to examine effects of growth factors released from leukocyte- and platelet-rich fibrin (L-PRF) and concentrated growth factor (CGF) on the osteogenic differentiation of periodontal ligament fibroblasts (PDLFs).MethodsLeukocyte- and platelet-rich fibrins, CGFs and PDLFs were obtained from New Zealand rabbits. The release of basic fibroblast growth factor (bFGF), bone morphogenetic protein 2 (BMP-2) and transforming growth factor β1 (TGF-β1) from L-PRFs and CGFs was measured at 5 h and 1, 3, 5, 7 days, using the enzyme linked immunosorbent assay. The PDLFs were treated with exudates of L-PRF or CGF. After the treatment, cell counting kit-8 assay was performed at day 1, 3, 5 and 7. Alkaline phosphatase (ALP) assay and Western blotting were applied at day 7. Three blocking antibodies were used to neutralize the proteins of bFGF, BMP-2 and TGF-β1.ResultsLeukocyte- and platelet-rich fibrin and CGF showed different growth factor release pattern, but similar accumulated concentration of these growth factors. PDLFs proliferation was significantly promoted by both L-PRF and CGF at day 1, 3 and 7, and CGF group was superior to L-PRF group at day 1 and 3. Both L-PRF and CGF significantly enhanced PDLFs ALP activity and protein expression of osteogenic markers. The osteopontin level was higher in CGF group than in L-PRF group, but no significant differences were found between two groups for ALP activity. Three blocking antibodies significantly downregulated both L-PRF and CGF induced osteogenic markers expression.ConclusionBoth CGF and L-PRF can promote the proliferation and osteogenic differentiation of PDLFs. The bFGF, BMP-2 and TGF-β1 are involved in both L-PRF and CGF induced osteogenic differentiation of PDLFs.

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Section: Discussionmentioning

confidence: 65%

Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study

Zhang

Zhao

et al. 2019

PeerJ

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“…Architecture and components of different scaffolds may provide more interface area for release or affect its cell attachment (Yu et al, ). Bonazza et al showed that the mixture of CGFs with β‐TCP could increase the release of BMP‐2 and BMP‐7 due to the presence of Ca ions in β‐TCP (Bonazza et al, ).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, a study on the release pattern of CGF in scaffolds, like deproteinized bovine bone mineral and intrafibrillar‐mineralized collagen (IMC), showed that mixture of CGF with IMC causes a sustained release of growth factors and cytokines until 28 days (Yu, Wang, Liu, & Qiao, ). It seems that addition of some biomaterials—like beta‐tricalcium phosphate (β‐TCP)—could enhance the release of some growth factors, like bone morphogenetic protein (BMP) 2 and 7, which have an important role in bone regeneration (Bonazza et al, ).…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo effects of concentrated growth factor on cells and tissues

Tabatabaei

Aghamohammadi

Tayebi

2020

J Biomedical Materials Res

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This article reviews the biological outcome of the concentrated growth factor (CGF), a new platelet derivative used for tissue regeneration, in published articles related to the use of this product in basic and clinical studies. An electronic literature research using PubMed and SCOPUS was performed using combination of keywords: “concentrated growth factor” (OR “CGF”), AND “stem cells,” AND “cells” OR “cell proliferation” OR “cell migration” OR “cell differentiation,” AND “repair” OR “survival” OR “revitalization,” AND “tissue” OR “bone.” Forty‐five articles that were published between 2012 and 2020 met the inclusion criteria. These studies have used CGF as fresh solid form, freeze‐dried, membrane, extract, or exudate. Most studies demonstrate the positive effects of CGF in a dose‐dependent manner under certain concentrations. Studies comparing CGF with other platelet concentrates, report lower efficiency, no statistically significant differences, or better results for CGF. Combination of CGF with stem cells and biomaterials significantly improves bone regeneration and the effect of allograft or collagen membrane is better than CGF alone. For a better examination of the biological outcomes of CGF, the standardization of CGF preparation regarding the choice of the test tube material for blood collection, the required volume of blood, the necessary count of platelets in CGF, and the most appropriate type of CGF are recommended.

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“…Cihaz içindeki kan tüpleri 30 saniye hızlanma, 2 dakika 2700 rpm (600 g), 4 dakika 2400 rpm (400 g), 4 dakika 2700 rpm (600 g), 3 dakika 3000 rpm ve 36 saniye yavaşlama içeren bir programla çalışıldı. İşlem sonunda 3 farklı kan fraksiyonu tanımlandı; 1) en üstte sıvı yapıda trombositten fakir plazma (PPP), 2) ortada daha katı kıvamda CGF, 3) en altta ise kırmızı kan hücrelerinden oluşan tabaka (12). Alınan orta CGF tabakası özel bir maşa yardımı ile sıkıştırılarak ince bir CGF matriks elde edildi (Şekil 1).…”

Section: Konsantre Büyüme Faktörü Hazırlanmasıunclassified

“…Fibroblast büyüme faktörü (FGF) granülasyon doku formasyonunda, reepitelizasyonda ve doku yeniden şekillendirilmesinde rol alır (1). İnsülin benzeri büyüme faktörü 1 (IGF-1), hücre apopitozunu geciktirir ve IGF-1 reseptörü endotelial cevapta önemli rol alır (12). Granüller içinde ayrıca trombosit kaynaklı anjiogenez faktörü (PDAF), trombosit faktör 4 (PF-4), keratinosit büyüme faktörü (KGF) ve interlökin 1 (IL-1) bulunmaktadır (8,11).…”

Section: Introductionunclassified

Konsantre büyüme faktörünün random patern cilt flep yaşayabilirliği üzerine etkisi: deneysel çalışma

Gürsoy

Koca

Alişik

et al. 2020

Journal of Health Sciences and Medicine

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Introduction: Random pattern skin flaps are used frequently for the reconstruction of random pattern skin defects from various causes in plastic and reconstructive surgery. Most frequent complication with these flaps is the necrosis of distal parts due to inadequate blood supply. Various types of techniques have been defined to avoid and reduce flap loss. The aim of this study is, for the first time, to evaluate the effect of concentrated growth factor (CGF), which is derived from whole blood and is rich in growth factors, on the survival of random pattern skin flaps. Material and Method: Fifty Sprague-Dawley rats were used for the study. Forty of them were used for the experiment group and ten for the preparation of CGF. Distal based 3x9 cm McFarlene flaps were elevated from the back of the rats and in CGF group (n:20), CGF matrix was placed before closure. No treatment was given to the control group (n:20). On postoperative day 7, the flaps were evaluated by photographic, scintigraphic, and histopathological methods. Results: The median rate of surviving flap areas in control group was 53% (47-58). In CGF group the median rate of flap viability was %58 (55-64). The higher flap viability rates obtained from CGF applied group was found to be statistically significantly higher from control group (p<0,001). For the scintigraphic perfusion area evaluations, the median rate of flap perfusion was 54% (51-60) for the control group and 61% (59-64) for the CGF group. The higher flap perfusion rates obtained from CGF applied group was found to be statistically significantly higher from control group (p=0,001). The median rate of vascularity scores in CGF treated group was 10,0 (8,25-11,75) and 7,5 (6,25-9,00) in control group. The difference was found to be statistically significantly higher in the study group (p=0,003). Inflammation scores were not found to be statistically significantly different (p=0,246). Conclusions: This study shows that locally applied CGF under skin flap increases flap viability which might be associated with the increase in angiogenesis. This study for the first time shows that in flap surgery, applying CGF which is rich in growth factors, might have favorable effects on the flaps that are prone to ischemia but further studies are needed.

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Growth Factors Release From Concentrated Growth Factors: Effect of β-Tricalcium Phosphate Addition

Abstract: The present data suggest that β-TCP addition to CGF could enhance and improve tissue regeneration, especially bone regeneration, increasing the release of some growth factors that play an important role in osteogenesis.

Cited by 26 publications

References 50 publications

Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study

Effect of autogenous growth factors released from platelet concentrates on the osteogenic differentiation of periodontal ligament fibroblasts: a comparative study

In vitro and in vivo effects of concentrated growth factor on cells and tissues

Konsantre büyüme faktörünün random patern cilt flep yaşayabilirliği üzerine etkisi: deneysel çalışma

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