The extracellular proteases produced by Vibrio parahaemolyticus

Osei‐Adjei, George; Huang, Xinxiang; Zhang, Yiquan

doi:10.1007/s11274-018-2453-4

Cited by 37 publications

(22 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Extracellular proteases are also one of the important virulence factors of bacteria. Osei‐Adjei et al () showed that extracellular protease from Vibrio parahaemolyticus is closely related to its pathogenicity. Luan et al () reported that zinc metalloprotease may cause tissue damage by directly degrading the host tissues and promoting pathogenesis through bacterial invasion.…”

Section: Discussionmentioning

confidence: 99%

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Mao

Qin

Kang

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

LuxR‐type transcriptional factors are essential in many bacterial physiological processes. However, there have been no reports on their roles in Aeromonas hydrophila. In this study, six stable silent strains were constructed using shRNA. Significant decreases in the expression levels of luxR05, luxR08, luxR19, luxR11, luxR164 and luxR165 were shown in their respective strains by qRT‐PCR. The luxR05‐RNAi and luxR164‐RNAi exhibit the most significant changes in sensitivity to kanamycin and gentamicin. The luxR05‐RNAi showed minimum biofilm formation and the least motility, while luxR164‐RNAi showed minimum biofilm formation, adhesion, growth and extracellular protease activity compared to the wild‐type strain. In summary, the results of this paper suggest that all six luxR genes are involved in multiple physiological processes in A. hydrophila and that the roles of luxR05 and luxR164 are highly significant. The sensitivity of luxR05‐RNAi and luxR164‐RNAi to drugs may be closely related to biofilm formation. The luxR05 may play an important role in the pathogenicity of A. hydrophila by regulating the movement, adhesion and biofilm formation of bacteria, whereas luxR164 may be involved in similar functions by regulating bacterial adhesion, extracellular enzyme activity and growth. These results help further our understanding of the drug resistance and pathogenesis of A. hydrophila.

show abstract

Section: Discussionmentioning

confidence: 99%

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Mao

Qin

Kang

et al. 2019

Journal of Fish Diseases

View full text Add to dashboard Cite

show abstract

“…However, PirA VP and PirB VP toxin mixture was reported to form complex and through receptor binding, oligomerization and pore formation, exhibits a higher toxic effect on experimental animals [ 19 , 28 , 38 , 53 ]. Although, PirA VP and PirB VP toxins are directly responsible for shrimp mortality during AHPND [ 33 , 59 ], several other pathogenic extracellular proteins (ECP) are identified in V. parahaemolyticus strains like hemin; enterobactin; vibrioferrin; type I, II and VI secretion system protein; chemotaxis protein (60 kDa); flagellin (40 kDa); metalloproteases (PrtV protein, 62 kDa; VppC protein, 90 kDa and VPM protein, 90 kDa); and serine proteases (VPP1, 43 kDa; VpSP37, 37 kDa and PrtA, 71 kda), which might contribute in toxicity of AHPND-causing bacteria [ 63 , 64 , 65 , 66 , 67 ]. For example, the AHPND pathology induced by 1 µg of crude protein (60% ammonium sulfate) precipitated from AHPND-causing V. parahaemolyticus broth culture, is equivalent to AHPND caused by 10 µg each of pure PirA VP and PirB VP toxins [ 28 , 37 ].…”

Section: Acute Hepatopancreatic Necrosis Disease (Ahpnd)—an Overviewmentioning

confidence: 99%

Acute Hepatopancreatic Necrosis Disease (AHPND): Virulence, Pathogenesis and Mitigation Strategies in Shrimp Aquaculture

Kumar

Roy

Behera

et al. 2021

Toxins

117

View full text Add to dashboard Cite

Shrimp, as a high-protein animal food commodity, are one of the fastest growing food producing sectors in the world. It has emerged as a highly traded seafood product, currently exceeding 8 MT of high value. However, disease outbreaks, which are considered as the primary cause of production loss in shrimp farming, have moved to the forefront in recent years and brought socio-economic and environmental unsustainability to the shrimp aquaculture industry. Acute hepatopancreatic necrosis disease (AHPND), caused by Vibrio spp., is a relatively new farmed penaeid shrimp bacterial disease. The shrimp production in AHPND affected regions has dropped to ~60%, and the disease has caused a global loss of USD 43 billion to the shrimp farming industry. The conventional approaches, such as antibiotics and disinfectants, often applied for the mitigation or cure of AHPND, have had limited success. Additionally, their usage has been associated with alteration of host gut microbiota and immunity and development of antibiotic resistance in bacterial pathogens. For example, the Mexico AHPND-causing V. parahaemolyticus strain (13-306D/4 and 13-511/A1) were reported to carry tetB gene coding for tetracycline resistance gene, and V. campbellii from China was found to carry multiple antibiotic resistance genes. As a consequence, there is an urgent need to thoroughly understand the virulence mechanism of AHPND-causing Vibrio spp. and develop novel management strategies to control AHPND in shrimp aquaculture, that will be crucially important to ensure food security in the future and offer economic stability to farmers. In this review, the most important findings of AHPND are highlighted, discussed and put in perspective, and some directions for future research are presented.

show abstract

“…Vibrio parahaemolyticus expresses extracellular metalloproteases-PrtV, VppC, and VPM-and extracellular serine proteases-VPP1, VpS37, and PrtA (Osei-Adjei et al, 2018). PrtV is a collagenase and can be inhibited by ethylenediaminetetraacetic acid (EDTA) (Yu et al, 2000), VppC acts in the early stationary phase of growth (Miyoshi et al, 2008), VPM is a putative virulence factor (Luan et al, 2007), VVP1 causes cytotoxicity and lethality in mice (Miyoshi, 2013), VpS37 exhibits gelatinolytic activity (Salamone et al, 2015), and PrtA causes abdominal hemorrhage in mice and is toxic to various mammal cells (Lee et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Quorum-Sensing Regulator OpaR Directly Represses Seven Protease Genes in Vibrio parahaemolyticus

Chang

Lee

2020

Front. Microbiol.

View full text Add to dashboard Cite

Proteases play a key role in numerous bacterial physiological events. Microbial proteases are used in the pharmaceutical industry and in biomedical applications. The genus Vibrio comprises protease-producing bacteria. Proteases transform polypeptides into shorter chains for easier utilization. They also function as a virulence factor in pathogens. The mechanism by which protease genes are regulated in Vibrio parahaemolyticus, an emerging worldwide human pathogen, however, still remains unclear. Quorum sensing is the communication system of bacteria. OpaR is the master quorumsensing regulator in V. parahaemolyticus. In the present study, quantitative reverse transcriptase-polymerase chain reaction and protease gene promoter-fusion reporter assays revealed that OpaR represses seven protease genes-three metalloprotease genes and four serine protease genes-which are involved in environmental survival and bacterial virulence. Furthermore, the electrophoresis mobility shift assay demonstrated that OpaR is bound directly to the promoter region of each of the seven protease genes. DNase I footprinting identified the sequence of these OpaR-binding sites. ChIPseq analyses revealed 435 and 835 OpaR-binding sites in the late-log and stationary phases, respectively. These OpaR-binding sequences indicated a conserved OpaRbinding motif: TATTGATAAAATTATCAATA. These results advance our understanding of the protease regulation system in V. parahaemolyticus. This study is the first to reveal the OpaR motif within V. parahaemolyticus in vivo, using ChIP-seq, and to provide a database for OpaR direct regulon.

show abstract

The extracellular proteases produced by Vibrio parahaemolyticus

Cited by 37 publications

References 70 publications

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Role of LuxR‐type regulators in fish pathogenic Aeromonas hydrophila

Acute Hepatopancreatic Necrosis Disease (AHPND): Virulence, Pathogenesis and Mitigation Strategies in Shrimp Aquaculture

Quorum-Sensing Regulator OpaR Directly Represses Seven Protease Genes in Vibrio parahaemolyticus

Contact Info

Product

Resources

About