Evaluation of the molecular lipid organization in millimeter‐sized stratum corneum by synchrotron X‐ray diffraction

Suzuki, Tomonori; Uchino, Tomonobu; Hatta, Ichiro; Miyazaki, Yasunori; Kato, S.; Sasaki, Kiyotaka; Kagawa, Yoshiyuki

doi:10.1111/srt.12474

Cited by 5 publications

(1 citation statement)

References 29 publications

(84 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, topical skin delivery methods for various intermediate- and high-molecular-weight compounds have been developed recently 5 , 39 . The intercellular lipids in the SC have been reported to have rich and poor domains of lamellar- and lateral-packing structures 40 , and the poor domains are thought to contribute to the penetration of intermediate- and high-molecular-weight compounds. In addition, the SC has two layers: the stratum compactum and the stratum disjunctum.…”

Section: Discussionmentioning

confidence: 99%

Biocompatible topical delivery system of high-molecular-weight hyaluronan into human stratum corneum using magnesium chloride

Fujii,

Okishima,

Ichiwata

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Non-invasive delivery of hyaluronan into the stratum corneum (SC) is extremely difficult because of its high molecular weight and the strong barrier of the SC. We developed a safe method of administering hyaluronan into the human SC and determined its penetration route. The amount of hyaluronan that penetrated into the SC was 1.5–3 times higher in the presence of magnesium chloride hexahydrate (MgCl2) than other metal chlorides. The root-mean-square radius of hyaluronan in water decreased with the addition of MgCl2. Moreover, MgCl2 solutions maintained their dissolved state on a plastic plate for a long time, suggesting that size compaction and inhibition of hyaluronan precipitation on the skin enhanced hyaluronan into the SC. Our results also strongly suggest that an intercellular route contributes to the penetration of hyaluronan from the upper to the middle layer of the SC. No disruption to the SC barrier was observed after continuous use once a day for 1 month, demonstrating the potential of our method for the safe, topical application of hyaluronan.

show abstract

Section: Discussionmentioning

confidence: 99%

Biocompatible topical delivery system of high-molecular-weight hyaluronan into human stratum corneum using magnesium chloride

Fujii,

Okishima,

Ichiwata

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

Study on the drug permeation mechanism from flurbiprofen-loaded glyceryl monooleyl ether-based lyotropic liquid crystalline nanoparticles across the skin: Synchrotron X-ray diffraction and confocal laser scanning microscopy study

Uchino

Kato

Hatta

et al. 2019

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Development of novel polyglycerol fatty acid ester-based nanoparticles for the dermal delivery of tocopherol acetate

Uchino

Fujimori

Hatta

et al. 2021

International Journal of Pharmaceutics

View full text Add to dashboard Cite

Evaluation of the molecular lipid organization in millimeter‐sized stratum corneum by synchrotron X‐ray diffraction

Abstract: We found that the selection of a sample piece with strong diffraction peaks due to the lamellar and lateral structure enabled evaluation of the SC structure in small-sized samples by X-ray diffraction.

Cited by 5 publications

References 29 publications

Biocompatible topical delivery system of high-molecular-weight hyaluronan into human stratum corneum using magnesium chloride

Biocompatible topical delivery system of high-molecular-weight hyaluronan into human stratum corneum using magnesium chloride

Study on the drug permeation mechanism from flurbiprofen-loaded glyceryl monooleyl ether-based lyotropic liquid crystalline nanoparticles across the skin: Synchrotron X-ray diffraction and confocal laser scanning microscopy study

Development of novel polyglycerol fatty acid ester-based nanoparticles for the dermal delivery of tocopherol acetate

Contact Info

Product

Resources

About