Complete Genome Sequences of Six Human Bocavirus Strains from Patients with Acute Gastroenteritis in the North Region of Brazil

Watanabe, Aripuanã Sakurada Aranha; Luchs, Adriana; Leal, Élcio; Milagres, Flavio Augusto de Pádua; Komninakis, Shirley Vasconcelos; Gill, Danielle Elise; Lobato, Márcia Cristina Alves Brito Sayão; Brustulin, Rafael; Chagas, Rogério Togisaki das; Abrão, Maria de Fátima Neves dos Santos; Soares, Cassia Vitória de Deus Alves; Deng, Xutao; Sabino, Éster Cerdeira; Delwart, Eric; Costa, Antônio Charlys da

doi:10.1128/genomea.00235-18

Cited by 11 publications

(8 citation statements)

References 7 publications

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“…Regarding the analysis of HBoV sequences, a similarity was observed with HBoV‐1 strains described in India and Brazil among children with gastroenteritis and pneumonia, respectively . Among the HBoV‐3 species, the similarity was observed with strains which circulated in the state of Tocantins in 2014, the current research period, as well as with strains from the respiratory tract reported in China …”

Section: Discussionmentioning

confidence: 53%

Prevalence of rotavirus and human bocavirus in immunosuppressed individuals after renal transplantation in the Northern Region of Brazil

Castro¹,

Calvet

Sousa

et al. 2019

Journal of Medical Virology

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Immunosuppressive therapy causes severe impairment of host defense and diarrhea is a frequent complication in renal transplant recipients. This study aimed to describe the occurrence of Rotavirus A (RVA) and Human Bocavirus (HBoV) in fecal samples of immunosuppressed patients submitted to renal transplantation during posttransplant follow‐up. A longitudinal study was carried out involving a 25‐patient cohort, selected for kidney transplantation. A total of 126 fecal samples were collected between May 2014 and May 2016. Molecular techniques were used to detect and characterize circulating RVA and HBoV genotypes and statistical analysis were applied to verify the association between epidemiological and clinical characteristics. The prevalence of RVA and HBoV was 24% (6/25) and 40% (10/25), respectively. Among RVA and HBoV positive cases, the majority was female; did not conduct water treatment nor had adequate sewage facilities. The most detected genotypes were RVA G3 (62.5%) and HBoV‐3 (95%). Phylogenetic analysis of HBoV strains indicated that studied samples were similar to those found in Asian and American countries. The present study point out the circulation of these viral agents among immunosuppressed individuals and these findings will enable the construction of new knowledge and care perspectives on the cause of diarrhea in this population.

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Section: Discussionmentioning

confidence: 53%

Prevalence of rotavirus and human bocavirus in immunosuppressed individuals after renal transplantation in the Northern Region of Brazil

Castro¹,

Calvet

Sousa

et al. 2019

Journal of Medical Virology

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“…The samples described above were initially sent to the Public Health Laboratory of Tocantins (LACEN-TO) and stored at −20 °C, and the frozen faecal specimens were then taken to USP's Institute of Tropical Medicine (IMT/USP) to identify common enteric viruses, including RVAs, NoVs, HAstVs [32], and HSaVs [33], as well as rare [34][35][36] or potential new viruses [37][38][39][40][41][42], through NGS investigation.…”

Section: Study Populationmentioning

confidence: 99%

Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics

Tahmasebi

Luchs

Tardy

et al. 2020

Journal of General Virology

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Human enteric adenovirus species F (HAdV-F) is one of the most common pathogens responsible for acute gastroenteritis worldwide. Brazil is a country with continental dimensions where continuous multiregional surveillance is vital to establish a more complete picture of the epidemiology of HAdV-F. The aim of the current study was to investigate the molecular epidemiology of HAdV-F using full-genome data in rural and low-income urban areas in northern Brazil. This will allow a genetic comparison between Brazilian and global HAdV-F strains. The frequency of HAdV-F infections in patients with gastroenteritis and molecular typing of positive samples within this period was also analysed. A total of 251 stool samples collected between 2010 and 2016 from patients with acute gastroenteritis were screened for HAdV-F using next-generation sequencing techniques. HAdV-F infection was detected in 57.8 % (145/251) of samples. A total of 137 positive samples belonged to HAdV-F41 and 7 to HAdV-F40. HAdV-F40/41 dual infection was found in one sample. Detection rates did not vary significantly according to the year. Single HAdV-F infections were detected in 21.9 % (55/251) of samples and mixed infections in 37.4 % (94/251), with RVA/HAdV-F being the most frequent association (21.5 %; 54/251). Genetic analysis indicated that the HAdV-F strains circulating in Brazil were closely related to worldwide strains, and the existence of some temporal order was not observed. This is the first large-scale HAdV-F study in Brazil in which whole-genome data and DNA sequence analyses were used to characterize HAdV-F strains. Expanding the viral genome database could improve overall genotyping success and assist the National Center for Biotechnology Information (NCBI)/GenBank in standardizing the HAdV genome records by providing a large set of annotated HAdV-F genomes.

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“…and Taenia solium ), using conventional cultivation techniques and parasitological tests, such as Hoffman’s method and fresh direct examination. The samples were stored at −20 °C and forwarded to the USP Institute of Tropical Medicine of the University of São Paulo (IMT-USP) for identification of gastroenteric viruses (rotavirus, norovirus, human adenovirus, human astrovirus, human bocavirus, and human sapovirus) [ 23 , 24 , 25 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis

Ramos

Rosa

Ribeiro

et al. 2021

Viruses

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Echoviruses (E) are a diverse group of viruses responsible for various pathological conditions in humans including aseptic meningitis, myocarditis, and acute flaccid paralysis. The detection and identification of echovirus genotypes in clinical samples is challenging due to its high genetic diversity. Here, we report the complete genome sequences of nine echoviruses, obtained by next-generation sequencing of 238 fecal samples from individuals with gastroenteritis in regions of Brazil. Detected viruses were classified into six genotypes: Three E1 sequences (BRA/TO-028, BRA/TO-069 and BRA/TO-236), one E3 (BRA/TO-018), one E11 (BRA/TO-086), one E20 (BRA/TO-016), two E29 (BRA/TO-030 and BRA/TO-193), and one E30 sequence (BRA/TO-032). Phylogenetic analysis indicated that the echoviruses E1 and E29 circulating in Brazil are divergent from strains circulating worldwide. The genotype diversity identified in our study may under-represent the total echovirus diversity in Brazil because of the small sample size and the restricted geographical distribution covered by the survey.

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Complete Genome Sequences of Six Human Bocavirus Strains from Patients with Acute Gastroenteritis in the North Region of Brazil

Cited by 11 publications

References 7 publications

Prevalence of rotavirus and human bocavirus in immunosuppressed individuals after renal transplantation in the Northern Region of Brazil

Prevalence of rotavirus and human bocavirus in immunosuppressed individuals after renal transplantation in the Northern Region of Brazil

Viral gastroenteritis in Tocantins, Brazil: characterizing the diversity of human adenovirus F through next-generation sequencing and bioinformatics

High Heterogeneity of Echoviruses in Brazilian Children with Acute Gastroenteritis

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