Pharmacological characterization of a β‐adrenergic‐like octopamine receptor in <i>Plutella xylostella</i>

Huang, Qingting; Ma, Haihao; Deng, Xile; Zhu, Hang; Liu, Jia; Zhou, Yong; Zhou, Xiaomao

doi:10.1002/arch.21466

Cited by 20 publications

(30 citation statements)

References 32 publications

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“…In the brown planthopper, Nilaparvata lugens, Octβ2R was found to be essential for oviposition and could activate the cAMP levels of the oviducts (Wu et al, 2017). However, phentolamine, a consistently potent antagonist of Octβ-Rs in P. xylostella (Huang et al, 2018) and other insect such as rice stem borer, Chilo suppressalis (Wu et al, 2012), locusts, L. migratoria (Evans, 1984;Lange and Orchard, 1986), and brown planthopper, N. lugens (Wu et al, 2017), had a weak inhibitory effect and needed to be administered at a higher concentration than mianserin and epinastine, which are the antagonists of both Octα-Rs and Octβ-Rs. It can be assumed that Octβ-Rs might not be the only and key receptors involved in P. xylostella oviposition and can be compensated by Octα-Rs.…”

Section: Discussionmentioning

confidence: 99%

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Role of Biogenic Amines in Oviposition by the Diamondback Moth, Plutella xylostella L

et al. 2020

Front. Physiol.

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Oviposition is an important reproductive behavior that is triggered by mating in insects, and biogenic amines might be involved in its regulation. The effects of biogenic amines on oviposition have only been studied in a few insect species, and the findings to date have not been conclusive. In addition, there are few studies on the effects of biogenic amines on oviposition of the diamondback moth, Plutella xylostella L. Here, we tested how mating and biogenic amines regulate oviposition of P. xylostella by injecting amines and amine receptor antagonists into virgin and mated females and counting the number of eggs laid afterward. Biogenic amines of octopamine and tyramine could induce virgin adults of P. xylostella to lay eggs, while dopamine and serotonin had no such effect on oviposition. Furthermore, the octopamine antagonists mianserin, epinastine, and phentolamine inhibited oviposition by mated females. The tyramine antagonist yohimbine, dopamine antagonist SCH23390, and serotonin antagonist ketanserin did not block oviposition by mated females, and octopamine and tyramineinducing oviposition by virgin females could be inhibited by the octopamine antagonists mianserin and epinastine instead of the tyramine antagonist yohimbine. We conclude that octopamine and its receptors are involved in mating-triggered oviposition in P. xylostella, while tyramine acts as a subsidiary. Further, the inducing effect of tyramine on oviposition is achieved via octopamine receptors instead of tyramine receptors. This experiment is helpful to further understand the role of biogenic amines in mating regulation and to provide a new strategy for controlling P. xylostella.

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Section: Discussionmentioning

confidence: 99%

“…The diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae), is a worldwide and destructive pest of cruciferous vegetables (Liu et al, 2008;Huang et al, 2018). This moth has the characteristics of a short life cycle, great fecundity, and notorious insecticide resistance (Gong et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Role of Biogenic Amines in Oviposition by the Diamondback Moth, Plutella xylostella L

et al. 2020

Front. Physiol.

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“…The conserved protein domains were analyzed using the CD-search service (using the NCBI Conserved Domain Database CDD v3.18–55570 PSSMs, , accessed on 4 January 2017). Kir channel amino acid sequences from different species were collected and their evolutionary tree was analyzed using the neighbor-joining method based on the Poisson modified model in MEGA7 software [ 37 ].…”

Section: Methodsmentioning

confidence: 99%

“…nih.gov/Structure/cdd/wrpsb.cgi, accessed on 4 January 2017). Kir channel amino acid sequences from different species were collected and their evolutionary tree was analyzed using the neighbor-joining method based on the Poisson modified model in MEGA7 software [37].…”

Section: Sequences Alignment and Phylogenetic Analysismentioning

confidence: 99%

Identification and Expression of Inward-Rectifying Potassium Channel Subunits in Plutella xylostella

Lai

et al. 2020

Insects

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In insects, inward-rectifying potassium (Kir) channels regulate vital physiological functions, such as feeding behavior, silk secretion, renal excretion, and immune function. Therefore, they offer promising potential as targets for insecticides. Three types of Kir subunits have been identified in Diptera and Hemiptera, but the Kir subunits of Lepidoptera still remain unclear. This study identified five Kir subunit genes (pxkir1, pxkir2, pxkir3A, pxkir3B, and pxkir4) in the transcriptome of Plutella xylostella. Phylogenetic analysis identified pxkir1, pxkir2, pxkir3A, and pxkir3B as orthologous genes of kir1–3 in other insects. Interestingly, pxkir4 may be encoding a new class of Kir subunit in Lepidoptera that has not been reported to date. To identify further Kir channel subunits of P. xylostella, the gene expression profiles of five pxkir genes were studied by quantitative real-time PCR. These pxkir genes are expressed throughout the development of P. xylostella. pxkir1 and pxkir2 were highly expressed in thoraxes and legs, while pxkir3 (3A and 3B) and pxkir4 had high expression levels in the midgut and Malpighian tubules. This study identified the composition and distribution of Kir subunits in P. xylostella for the first time, and provides useful information for the further study of Kir channel subunits in Lepidoptera.

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“…Functional analyses through in vitro expression of β-adrenergic-like octopamine receptor in Plutella xylostella, Huang et al found that amitraz, octopamine, and tyramine all activate the β-adrenergic-like octopamine receptor, leading to elevated cyclic AMP levels. An expression pattern study indicated that the β-adrenergic-like octopamine receptor is involved in nearly every growth stage of P. xylostella (Huang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Genetic analysis and screening of detoxification-related genes in an amitraz-resistant strain of Panonychus citri

Cong

Liu

et al. 2020

Bull. Entomol. Res.

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Panonychus citri (McGregor) is the most common pest in citrus-producing regions. Special low-toxicity acaricides, such as spirocyclic tetronic acids and mite growth inhibitors, have been used for a long time in China. However, pesticide resistance in mites is a growing problem due to the lack of new acaricide development. Wide-spectrum insecticides, such as amitraz have gained acceptance among fruit growers. An amitraz-resistant strain of P. citri was obtained by indoor screening to examine field resistance monitoring of mites to acaricides and to explore the resistant mechanism of mites against amitraz. The amitraz-resistant strain of P. citri had an LC50 value of 2361.45 mg l−1. The resistance ratio was 81.35 times higher in the resistant strain of P. citri compared with the sensitive strain. Crossing experiments between the sensitive and resistant strains of P. citri were conducted, resulting in a D value of 0.11 for F1 SS♀×RS♂ and 0.06 for F1 RS♀×SS♂. Reciprocal cross experiments showed that the dose–mortality curves for the F1 generations coincided, indicating that the resistance trait was not affected by cytoplasmic inheritance. The dose–expected response relationship was evaluated in the backcross generation and a significant difference was observed compared with the actual value. The above results indicate that the inheritance of resistance trait was incompletely dominant, governed by polygenes on the chromosome. Synergism studies demonstrated that cytochrome P450s and esterase may play important roles in the detoxification of amitraz. Based on differential gene analysis, 23 metabolism-related genes of P. citri were identified, consistent with the results of synergism studies. Real-time PCR verification implied that P450s, ABC transporters, and acetylcholinesterase might influence the detoxification of amitraz by P. citri. These results provide the genetic and molecular foundation for the management of pest mite resistance.

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Pharmacological characterization of a β‐adrenergic‐like octopamine receptor in Plutella xylostella

Cited by 20 publications

References 32 publications

Role of Biogenic Amines in Oviposition by the Diamondback Moth, Plutella xylostella L

Role of Biogenic Amines in Oviposition by the Diamondback Moth, Plutella xylostella L

Identification and Expression of Inward-Rectifying Potassium Channel Subunits in Plutella xylostella

Genetic analysis and screening of detoxification-related genes in an amitraz-resistant strain of Panonychus citri

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