Recent development in mass spectrometry and its hyphenated techniques for the analysis of medicinal plants

Zhu, Mingzhi; Chen, Guilin; Wu, Jian‐Lin; Li, Na; Z, Liu; Chen, Guofang

doi:10.1002/pca.2763

Cited by 33 publications

(29 citation statements)

References 82 publications

(141 reference statements)

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“…The utility of IM‐MS has been explored in a continuation of our research into profiling Passiflora species, the approach provides an additional strategy to the 2D separation techniques discussed by Zhu et al . and Cao et al .…”

Section: Resultsmentioning

confidence: 99%

“…Zhu et al . performed a comprehensive review in the use of MS and hyphenated techniques in the analysis of medicinal plants, one‐dimensional, multi‐dimensional, two‐dimensional (2D) LC‐MS/GC‐MS and direct analysis techniques were discussed, each technique was determined to be indispensable (where the most suitable MS technique should be selected according to the aim of the experiment) . Cao et al .…”

Section: Introductionmentioning

confidence: 99%

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Use of ion mobility mass spectrometry to enhance cumulative analytical specificity and separation to profile 6‐C/8‐C‐glycosylflavone critical isomer pairs and known–unknowns in medicinal plants

McCullagh

Pereira

Yariwake

2019

Phytochemical Analysis

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Introduction Plant medicine/herbal extracts are typically complex, encompassing a wide range of flavonoid diversity and biological benefits. Combined with a lack of standards; species authentication profiling is a challenge. A non‐targeted screening strategy using two‐dimensional (2D) separation and specificity of ultra‐high‐performance liquid chromatography ion mobility collision‐induced dissociation mass spectrometry (UHPLC‐IM‐CID‐MS) has been investigated, to identify the 6‐C and 8‐C‐glycosylflavone isomer orientin/isoorientin and vitexin/isovitexin pairs in Passiflora species. Utilising available standards and “known–unknowns” a reference CCS (collision cross‐section) speciation finger print for Passiflora extracts could be generated to illustrate species profiling. Material and Methods SPE was performed to extract flavonoids of interest from powdered and ground Passiflora leaf. Chromatographic separation was achieved via UHPLC and analysis performed using positive/negative ion electrospray coupled with linear T‐wave IM‐MS (calibrated to perform accurate mass and CCS measurements). Results Comparative phytochemical screening of Passiflora alata, P. edulis, P. incarnata and P. caerulea leaf extracts has generated CCS, CID IM product ion spectra, 2D separation with UHPLC‐IM‐MS, enabling the unequivocal identification of flavone C‐glycosides in complex extracts. A phytochemical reference CCS library was generated comprised of “knowns” and “known–unknowns”. Isomers have been differentiated using a CCS metric enabling novel CCS specific isomeric quantitation of co‐eluting isomers. Conclusions The screening approach illustrated has the potential to play an important role in the profiling of medicinal plants to determine phytochemical make‐up and improve consumer safety through generation of highly specific speciation profiles.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Use of ion mobility mass spectrometry to enhance cumulative analytical specificity and separation to profile 6‐C/8‐C‐glycosylflavone critical isomer pairs and known–unknowns in medicinal plants

McCullagh

Pereira

Yariwake

2019

Phytochemical Analysis

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“…As a matter of fact, however, there are still few reports about the potentials of polyphenols in R. prinoides in Kenya for antioxidants and their related activities. Today, more than 60% of the population in South Africa consumes traditional herbal medicines, and still up to 1.5 billion people worldwide consult the medicinal plants as their primary health care, especially in rural areas of developing countries [22]. Hence, with the growing interests in traditional herb medicines worldwide, the present study aims to comprehensively explore the antioxidant and anti-inflammatory characteristics and the polyphenol profile of the traditional ethno-medicine, R. prinoides in Kenya, and then further provide practical guidance for quality control (QC) and its daily and clinical usages as valuable research resource for the development of new natural pharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant, Anti-inflammatory Activities and Polyphenol Profile of Rhamnus prinoides

Chen

Mutie

et al. 2020

Pharmaceuticals

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Rhamnus prinoides L’Herit (R. prinoides) has long been widely consumed as folk medicine in Kenya and other Africa countries. Previous studies indicated that polyphenols were abundant in genus Rhamnus and exhibited outstanding antioxidant and anti-inflammatory activities. However, there are very few studies on such pharmacological activities and the polyphenol profile of this plant up to now. In the present study, the antioxidant activities of the crude R. prinoides extracts (CRE) and the semi-purified R. prinoides extracts (SPRE) of polyphenol enriched fractions were evaluated to show the strong radical scavenging effects against 1,1-diphenyl-2- picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) (0.510 ± 0.046 and 0.204 ± 0.005, mg/mL), and 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) (0.596 ± 0.005 and 0.096 ± 0.004, mg/mL), respectively. Later, the SPRE with higher contents of polyphenols and flavonoids displayed obvious anti-inflammatory activities through reducing the NO production at the dosage of 11.11 − 100 μg/mL, and the COX-2 inhibitory activity with an IC50 value at 20.61 ± 0.13 μg/mL. Meanwhile, the HPLC-UV/ESI-MS/MS analysis of polyphenol profile of R. prinoides revealed that flavonoids and their glycosides were the major ingredients, and potentially responsible for its strong antioxidant and anti-inflammatory activities. For the first time, the present study comprehensively demonstrated the chemical profile of R. prinoides, as well as noteworthy antioxidant and anti-inflammatory activities, which confirmed that R. prinoides is a good natural source of polyphenols and flavonoids, and provided valuable information on this medicinal plant as folk medicine and with good potential for future healthcare practice.

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“…Modern analytical instruments, especially the chromatography tandem mass spectrometry techniques, are capable of analyzing a large number of target compounds in a single analysis [5]. However, sample preparation procedure is still the crucial variable of multi-analyte analysis in achieving complete and accurate information [6].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Partition Efficiencies of Multiple Phenolic Compounds Contained in Propolis in Different Modes of Acetonitrile–Water-Based Homogenous Liquid–Liquid Extraction

Chen

et al. 2019

Molecules

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Homogeneous liquid–liquid extraction (HLLE) has attracted considerable interest in the sample preparation of multi-analyte analysis. In this study, HLLEs of multiple phenolic compounds in propolis, a polyphenol-enriched resinous substance collected by honeybees, were performed for improving the understanding of the differences in partition efficiencies in four acetonitrile–water-based HLLE methods, including salting-out assisted liquid–liquid extraction (SALLE), sugaring-out assisted liquid–liquid extraction (SULLE), hydrophobic-solvent assisted liquid–liquid extraction (HSLLE), and subzero-temperature assisted liquid–liquid extraction (STLLE). Phenolic compounds were separated in reversed-phase HPLC, and the partition efficiencies in different experimental conditions were evaluated. Results showed that less-polar phenolic compounds (kaempferol and caffeic acid phenethyl ester) were highly efficiently partitioned into the upper acetonitrile (ACN) phase in all four HLLE methods. For more-polar phenolic compounds (caffeic acid, p-coumaric acid, isoferulic acid, dimethoxycinnamic acid, and cinnamic acid), increasing the concentration of ACN in the ACN–H2O mixture could dramatically improve the partition efficiency. Moreover, results indicated that NaCl-based SALLE, HSLLE, and STLLE with ACN concentrations of 50:50 (ACN:H2O, v/v) could be used for the selective extraction of low-polarity phenolic compounds. MgSO4-based SALLE in the 50:50 ACN–H2O mixture (ACN:H2O, v/v) and the NaCl-based SALLE, SULLE, and STLLE with ACN concentrations of 70:30 (ACN:H2O, v/v) could be used as general extraction methods for multiple phenolic compounds.

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Recent development in mass spectrometry and its hyphenated techniques for the analysis of medicinal plants

Cited by 33 publications

References 82 publications

Use of ion mobility mass spectrometry to enhance cumulative analytical specificity and separation to profile 6‐C/8‐C‐glycosylflavone critical isomer pairs and known–unknowns in medicinal plants

Use of ion mobility mass spectrometry to enhance cumulative analytical specificity and separation to profile 6‐C/8‐C‐glycosylflavone critical isomer pairs and known–unknowns in medicinal plants

Antioxidant, Anti-inflammatory Activities and Polyphenol Profile of Rhamnus prinoides

Comparison of the Partition Efficiencies of Multiple Phenolic Compounds Contained in Propolis in Different Modes of Acetonitrile–Water-Based Homogenous Liquid–Liquid Extraction

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