Punctual mutations in<i>23S rRNA</i>gene of clarithromycin-resistant<i>Helicobacter pylori</i>in Colombian populations

Matta, Andrés Jenuer; Ríos, Diana Carolina Zambrano; Pazos, Alvaro J.

doi:10.3748/wjg.v24.i14.1531

Cited by 27 publications

(18 citation statements)

References 23 publications

(38 reference statements)

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“…Therefore, alternative medication regimens (e.g., quadruple therapy or modified triple regimens) should be considered for effective eradication therapy in this area. Clarithromycin resistance is associated with point mutations of the V domain of 23S rRNA in the 50S subunit that decreases the affinity of drug binding to the ribosome [4,6]. In our study, A2143G was the most common type of mutation in strains.…”

Section: Discussionmentioning

confidence: 56%

“…In the case of untreated and prolonged infection, H. pylori can cause peptic ulcer disease (PUD) and chronic gastritis (CG). Furthermore, neoplastic processes such as mucosa-associated lymphoid tissue (MALT) and gastric cancer (GC) are associated with this microorganism [3,4]. The International Agency for Research on Cancer classified H. pylori as class I carcinogen in 1994 [5].…”

Section: Introductionmentioning

confidence: 99%

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Molecular Assessment of Resistance to Clarithromycin in Helicobacter pylori Strains Isolated from Patients with Dyspepsia by Fluorescent In Situ Hybridization in the Center of Iran

Vazirzadeh

Falahi

Moghim

et al. 2020

BioMed Research International

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Background and Aims. Helicobacter pylori is a common infectious bacterium mostly found in gastroduodenal diseases. The increased prevalence of clarithromycin-resistant H. pylori strains is a major challenge in the successful treatment of infections caused by this organism. The present study is aimed at detecting the clarithromycin resistance pattern of H. pylori strains isolated from gastric biopsies and evaluating point mutations of the 23S rRNA gene. Patients and methods. In the present descriptive cross-sectional study, 165 patients with gastrointestinal disorders, who were referred to the Endoscopy Center of Dr. Shariati Hospital of Isfahan, Iran, were enrolled from April to July 2018. H. pylori infection was diagnosed by culture, and susceptibility of the isolates to clarithromycin was assessed by the E-test. Minimum inhibitory concentration (MIC) values were obtained based on EUCAST recommendations. Also, fluorescence in situ hybridization (FISH) was used to determine point mutations associated with clarithromycin resistance. Results. By using culturing, H. pylori was isolated from 50.3% (83/165) gastric biopsy specimens. The overall frequency of resistance to clarithromycin was 25.3% (21/83) by the E-test. In the resistance genotypic analysis, 19 isolates had mutations. The prevalence of A2143G and A2144G mutations was 68.4% (13/19) and 31.5% (6/19), respectively. A2143C mutation was not tracked in any isolate. Two isolates with MIC > 0:5 μg/mL had no mutations that could be related to other mechanisms of resistance. Conclusion. As presented in the study, the high prevalence of clarithromycin-resistant H. pylori due to point mutations of the 23S rRNA gene indicates the necessity of revising the standard treatment regimen based on antibiotic susceptibility pattern of each region.

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Section: Discussionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

Molecular Assessment of Resistance to Clarithromycin in Helicobacter pylori Strains Isolated from Patients with Dyspepsia by Fluorescent In Situ Hybridization in the Center of Iran

Vazirzadeh

Falahi

Moghim

et al. 2020

BioMed Research International

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“…clarithromycin-resistant 23srRNA (Matta et al, 2018), rpmJ, hp0605, hp0971 (Iwamoto et al, 2014), acrB (Binh et al, 2014) Campylobacter spp.…”

Section: Helicobacter Pylorimentioning

confidence: 99%

Mechanism and challenges associated with adaptation and evolution of drug-resistant bacteria: an overview

Kapil

Kumar

Sharma

2020

APJMBB

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Antimicrobial resistance is one of the leading challenges in the human healthcare segment. Advances in antimicrobial resistance studies have revealed various intrinsic, adaptive or acquired factors to be involved for pathogenicity. Antimicrobial agents are either bactericidal or bacteriostatic in action and prescribed according to the mode of action. Various factors are confined for the antimicrobial activity of these agents via biochemical, mechanical, physiological and molecular mechanisms. Microbial cell expresses a number of alternates responsible for the evolution of resistance against these agent activities involving cell surface modifications, enzyme inhibitions, modifications in efflux system, protein carriers and mutations in nucleic acids. Apart from this, the successful adaptations of such microbes have also been observed with the transfer of responsible genes through miscellaneous operations such as vertical evolution, horizontal gene transfer, co-selection, compensatory and random mutation. In addition, alterations or modifications in biochemical and physiological mechanisms at cellular levels are also responsible for antibiotic resistance. This article briefly shows the present scenario of antimicrobial resistance and the alternatives to overcome this global issue in future.

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“…At present, the mechanism of antibiotic resistance of Hp is not completely understood [10][11] . It is widely accepted that the resistance to these antimicrobials is related to mutations in Hp gene: clarithromycin-resistant strains present three-point mutations in the region of domain V of 23S ribosomal RNA (rRNA): A2142G, A2142C and A2143G [12][13] . In addition to the mutations, the e ux pump cluster is also involved in the development of resistance to clarithromycin [14][15] .…”

Section: Introductionmentioning

confidence: 99%

Analysis of Drug Resistance in Helicobacter Pylori by Complete Genome Sequencing in Bama County, Guangxi, China

YanQiang¹,

Huang

et al. 2020

Preprint

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Background: The resistance rate of clinical Helicobacter pylori (Hp) isolates has increased, however, the mechanism of drug resistance is unclear. In this study, we isolated drug-resistant Hp strains isolated from different areas and different populations of China for genomic analysis.Objectives: The aim of this study was to investigate drug resistance in Hp from Bama County, Guangxi, China.Methods: Minimal inhibitory concentrations (MICs) of clarithromycin, metronidazole and levofloxacin were determined and complete genome sequencing was performed with annotation. The presence of hp1181 and hp1184 genes was detected by RT-PCR. The relationships between hp1181, hp1184 and clarithromycin resistance were confirmed by gene mutation and drug-resistant strains. Results: Three drug-resistant Hp strains were isolated from patients with gastritis in Bama County. The strains showed a high degree of homology with hp26695 through complete genome detection and identification. Differences in genome sequences, gene quantity and gene characteristics were detected amongst the three strains. Prediction and analysis of the function on drug-resistant genes indicated that the RNA expression of hp1181 and hp1184 increased in the three strains that were the same in the artificially induced clarithromycin-resistant bacteria. After gene knockout, the drug sensitivity of the strains increased significantly.Conclusions: The expressions of the genes hp1184 and hp1181 were associated with clarithromycin resistance in the Hp from Bama, Guangxi.

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Punctual mutations in23S rRNAgene of clarithromycin-resistantHelicobacter pyloriin Colombian populations

Cited by 27 publications

References 23 publications

Molecular Assessment of Resistance to Clarithromycin in Helicobacter pylori Strains Isolated from Patients with Dyspepsia by Fluorescent In Situ Hybridization in the Center of Iran

Molecular Assessment of Resistance to Clarithromycin in Helicobacter pylori Strains Isolated from Patients with Dyspepsia by Fluorescent In Situ Hybridization in the Center of Iran

Mechanism and challenges associated with adaptation and evolution of drug-resistant bacteria: an overview

Analysis of Drug Resistance in Helicobacter Pylori by Complete Genome Sequencing in Bama County, Guangxi, China

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