Toxicogenetic study of omeprazole and the modulatory effects of retinol palmitate and ascorbic acid on Allium cepa

Braga, Antonio Lima; Meneses, Ag-Anne Pereira Melo de; Santos, José Victor de Oliveira; Reis, Antonielly Campinho dos; Lima, Róbson Borges de; Mata, Ana M.; Paz, Márcia Fernanda Correia Jardim; Alves, Leane Brunelle dos Santos; Shaw, Subrata; Uddin, Shaikh Jamal; Rouf, Razina; Das, Asish Kumar; Dev, Shrabanti; Shil, Manik Chandra; Shilpi, Jamil A.; Khan, Ishaq N.; Islam, Muhammad Torequl; Ali, Eunüs S.; Mubarak, Mohammad S.; Mishra, Siddhartha; Sousa, João Marcelo de Castro e; Melo‐Cavalcante, Ana Amélia de Carvalho

doi:10.1016/j.chemosphere.2018.04.021

Cited by 16 publications

(8 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compounds. New thiazole derivatives N(5benzyl1,3thiazol2yl)3,5dimethyl 1-benzofuran-2-carboxamide (compound 1) [6] and 8methyl2Me7[3CFphenilme thyl]pyrazolo [4,3e] [1,3]thiazolo [3,2a]pyrimidin4(2H)ones (compound 2) [7] were synthesized at the Department of Organic Chemistry of Ivan Franko National University of Lviv, Ukraine. 10 mM stock solutions were prepared in the Dimethyl sulfoxide (DMSO, SigmaAldrich, USA), and the working solutions were prepared using distilled water.…”

Section: Methodsmentioning

confidence: 99%

“…Anatelophase test in the mitotic root cells of Allium cepa is widely used plant bioassay in the genotoxicity studies. This test has been applied to investigate genotoxicity of different chemicals [3,11,14,15], anticancer drugs, for example, cisplatin, 5fluorouracil, imatinib mesylate [17], gambogic acid [20], alkylating agents (ethyl methanesulphonate), and methyl methanesulphonate [5,14], food synthetic flavors [2] and nanoparticles [4,12,13,16]. Bagatini et al also used Allium cepa test to evaluate genotoxic effects of hospital effluent in Brasil.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of new derivatives of 2-amino-5-benzylthiazole of genotoxicity and acute toxicity in Allium bioassays

et al. 2018

View full text Add to dashboard Cite

2 (1 and 10 µM). The compounds 1 (10 µM) and 2 (1 and 10 µM) did not affect the mitotic and phase (prophase, metaphase, anaphase, telophase) indices. A commercial anticancer drug Doxorubicin (0.1 and 1 µM) possessed a significant inhibitory effect on root growth and seed germination, mitotic index and enhanced a level of chromosomal aberrations in Allium cepa. The compound 1 at 10 µM and compound 2 at 1 µM and 10 µM did not possess a significant acute toxicity (inhibition of cell division, seed germination and growth of Allium roots), did not demonstrated the genotoxic effects (induction of chromosomal aberrations) in Allium bioassay. These results give primary evidence about a possibility of using the synthetic 2-amino-5-benzylthiazole derivativescompounds 1 and 2-as novel antineoplastic agents that will have no negative side effects in the treated plant organism. Additional experiments should be performed in order to evaluate the adverse effects of new derivatives of 2-amino-5-benzylthiazole in a vide spectrum of the concentrations for the prediction of environmental toxicity and genotoxicity of chemicals.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of new derivatives of 2-amino-5-benzylthiazole of genotoxicity and acute toxicity in Allium bioassays

et al. 2018

View full text Add to dashboard Cite

show abstract

“…In non-clinical studies, OME toxicogenic effects were identified in plant cells ( Allium cepa) [ 13 ], as well as Saccharomyces cerevisiae and murine Sarcoma 180 cells [ 75 ]. Thus, based in previous investigations, the present study aimed to evaluate cytogenetic damages in patients whith gastritis undergoing OME therapy, with and without H. pylori co-infection, through micronucleus test and expression of catalase and superoxide dismutase.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of mutagenesis, necrosis and apoptosis induced by omeprazole in stomach cells of patients with gastritis

et al. 2022

Self Cite

View full text Add to dashboard Cite

Background Gastritis is a superficial and prevalent inflammatory lesion that is considered a public health concern once can cause gastric ulcers and gastric cancer, especially when associated with Helicobacter pylori infection. Proton pump inhibitors, such as omeprazole, are the most widely used drugs to treat this illness. The aim of the study was evaluate cytogenetic effects of omeprazole in stomach epithelial cells of patients with gastritis in presence and absence of H. pylori, through cytogenetic biomarkers and catalse and superoxide dismutase analysis. Methods The study included 152 patients from the Gastroenterology Outpatient Clinic of Hospital Getúlio Vargas, Teresina—Brazil, that reported continuous and prolonged omeprazole use in doses of 20, 30 and 40 mg/kg. The participants were divided into groups: (1) patients without gastritis (n = 32); (2) patients without gastritis but with OME use (n = 24); (3) patients with gastritis (n = 26); (4) patients with gastritis undergoing OME therapy (n = 26); (5) patients with gastritis and H. pylori (n = 22) and (6) patients with gastritis and H. pylori on OME therapy (n = 22). Results OME induced cytogenetic imbalance in the stomach epithelium through the formation of micronuclei (group 6 > 1, 2, 3, 4, 5; group 5 > 1, 2, 3; group 4 > 1, 2, 3); bridges (groups 4 and 6 > 1, 2, 3, 5 and group 2 > 3, 5); buds (groups 2,4,6 > , 1, 3, 5); binucleated cells (group 6 > 1, 2, 3, 4, 5; group 4 > 1, 2, 3); (groups 2 and 3 > 1); picnoses (group 6 > 1, 2, 3, 4, 5), groups 2 and 5 > 1, 3; group 4 > 1, 2, 3, 5); cariorrexis (groups 6 and 4 > 1, 2, 3, 5; groups 2, 3, 5 > 1) and karyolysis (groups 2, 4, and 6 > 1, 3, 5; groups 3 and 5 > 1). The OME cytogenetic instability was associated with H. pylori infection, indicating clastogenic/aneugenic effects, chromosomes alterations, gene expression changes, cytotoxicity and apoptosis. Conclusions The cytogenetic changescan be attributed to several mechanisms that are still unclear, including oxidative damage, as observed by increased catalase and superoxide dismutase expresion. Positive correlations between antioxidant enzymes were found with micronuclei formation, and were negative for picnoses. Thus, the continuous and prolonged omeprazole use induces genetic instability, which can be monitored through cytogenetic analyzes, as precursor for gastric cancer.

show abstract

“…In non-clinical studies, toxicogenic effects of OME were identi ed in Allium cepa (Braga et al, 2018), as well as oxidative effects in Saccharomyces cerevisiae and cytogenetics in Sarcoma 180 cells (Paz et al, 2019). Thus, in continuity with the previousinvestigations, the present study aimed to evaluate the possible cytogenetic damages of the use and/or therapy of OME in the gastric epithelium of patients with gastritis, with and without H. pylori infection, by the application of the micronucleus test and dosages of catalase and superoxide dismutase.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Mutagenesis, Necrosis and Apoptosis induced by Omeprazole in Stomach Cells of Patients with Gastritis

Mata

Paz²,

Menezes³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Background: Gastritis is a superficial and prevalent inflammatory lesion that is considered a public health concernoncecause gastric ulcers and gastric cancer, especially due to Helicobacter pylori infection. Proton pump inhibitors, such as omeprazole, are the most widely used drugs in order to treat this illness. The aim of the study was evaluating the cytogenetic effects of omeprazole in stomach epithelial cells of patients with gastritis in presence and absence of H. pylori, using to this the application of cytogenetic biomarkers and measurements of catalse and superoxide dismutase. Methods: The study included 152 patients from the Gastroenterology Outpatient Clinic of Hospital Getúlio Vargas, Teresina - PI, that reported the continuous and prolonged use of omeprazole in doses of 20, 30 and 40 mg/kg. The participants were divided into groups: (1) patients without gastritis (n = 32); (2) patients without gastritis but with use of OME (n = 24); (3) patients with gastritis (n = 26); (4) patients with gastritis undergoing OME therapy (n = 26); (5) patients with gastritis and H. pylori (n = 22) and (6) patients with gastritis and H. pylori on OME therapy (n = 22). Results: OME induces cytogenetic risks in the epithelium of the stomach due to the formation of micronuclei (group 6> 1,2,3,4,5; group 5> 1,2,3; group 4> 1,2,3); bridges (groups 4 and 6> 1,2,3,5 and group 2> 3,5); buds (groups 2,4,6>, 1,3,5); binucleated cells (group 6> 1,2,3,4,5; group 4> 1,2,3); groups 2 and 3> 1); picnoses (group 6> 1,2,3,4,5), groups 2 and 5> 1,3; group 4> 1,2,3,5); cariorrexis (groups 6 and 4> 1,2,3,5; groups 2,3,5> 1) and karyolysis (groups 2,4, and 6> 1,3,5; groups 3 and 5> 1). These data show that omeprazole induces cytogenetic risks, especially due to infection with H. pylori, thus indicating the clastogenic and/or aneugenic effects, chromosomes changing, gene expression, cytotoxicity and apoptosis. Conclusions: These risks can be attributed to several mechanisms that are still unclear, including oxidative damage, as observed by increases in catalase and superoxide dismutase. Positive correlations between these antioxidant enzymes were obtained with the formation of micronuclei, and negative for picnoses. Thus, the continuous and prolonged use of omeprazole induces genetic instability, which can be monitored, in cytogenetic analyzes, as anticipation for cancer, especially gastric.

show abstract

Toxicogenetic study of omeprazole and the modulatory effects of retinol palmitate and ascorbic acid on Allium cepa

Cited by 16 publications

References 53 publications

Effects of new derivatives of 2-amino-5-benzylthiazole of genotoxicity and acute toxicity in Allium bioassays

Effects of new derivatives of 2-amino-5-benzylthiazole of genotoxicity and acute toxicity in Allium bioassays

Evaluation of mutagenesis, necrosis and apoptosis induced by omeprazole in stomach cells of patients with gastritis

Evaluation of Mutagenesis, Necrosis and Apoptosis induced by Omeprazole in Stomach Cells of Patients with Gastritis

Contact Info

Product

Resources

About