Effects of new derivatives of 2-amino-5-benzylthiazole of genotoxicity and acute toxicity in Allium bioassays

Abstract

2 (1 and 10 µM). The compounds 1 (10 µM) and 2 (1 and 10 µM) did not affect the mitotic and phase (prophase, metaphase, anaphase, telophase) indices. A commercial anticancer drug Doxorubicin (0.1 and 1 µM) possessed a significant inhibitory effect on root growth and seed germination, mitotic index and enhanced a level of chromosomal aberrations in Allium cepa. The compound 1 at 10 µM and compound 2 at 1 µM and 10 µM did not possess a significant acute toxicity (inhibition of cell division, seed germination and growth of Allium roots), did not demonstrated the genotoxic effects (induction of chromosomal aberrations) in Allium bioassay. These results give primary evidence about a possibility of using the synthetic 2-amino-5-benzylthiazole derivativescompounds 1 and 2-as novel antineoplastic agents that will have no negative side effects in the treated plant organism. Additional experiments should be performed in order to evaluate the adverse effects of new derivatives of 2-amino-5-benzylthiazole in a vide spectrum of the concentrations for the prediction of environmental toxicity and genotoxicity of chemicals.

Genotoxicity and acute toxicity of 2-amino-5-benzylthiazole in complex with polymeric nanocarrier in Allium bioassay

“…Inhibition of mitotic activity is one of the parameters for evaluating the cytotoxicity of various compounds. N. Finiuk et al (2018) show that Doxorubicin at 1 µM significantly reduced the indices of prophase, metaphase, anaphase and telophase. Thus, it was reconfirmed that anticancer drugs inhibit Allium cepa cell division.…”

“…There were no significant changes in the value of the chromosomal aberrations at the action of Th2 complex or polymeric carrier Th1. It should be noted that sodium azide and Doxorubicin (positive control agents) induced much more significant changes in the values of the mitotic index and chromosomal aberrations (Finiuk et al, 2018).…”

“…It was known, that BF1 (10 mM) in concentration equal to the IC 50 for tumor cells did not possess acute toxicity towards Allium cepa, while a significant inhibition of root growth and seed germination effects were detected at using BF1 only in the dose that is 10 times higher than IC 50 for tumor cells (Finiuk et al, 2018).…”

“…It was found that new derivatives of N-(5-benzyl-1,3-thiazol-2-yl)-3,5-dimethyl-1benzofuran-2-carboxamide (BF1) possess cytotoxic action towards human tumor cells ISSN 1996-4536 (print) (Finiuk et al, 2018). On the other hand, BF1 conjugated with novel polymeric nanoparticles based on polyethylene glycol (PEG-PN) to increase its solubility exhibited a higher level of cytotoxicity towards specific tumor cell lines than the pure (unconjugated) thiazole derivative or/and Doxorubicin (positive control) (Finiuk et al, 2021).…”