Expression of the human UDP-galactose transporter gene hUGT1 in tobacco plants' enhanced plant hardness

Abedi, Tayebeh; Mohamed, Khalil; Koike, Kanae; Hagura, Yoshio; Tazoe, Yuma; Ishida, Nobuhiro; Kitamura, Kenji; Tanaka, Nobukazu

doi:10.1016/j.jbiosc.2018.03.002

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…Preparation of cross-sections was performed as described previously with minor modifications ( Nitta et al , 2009 ; Abedi et al , 2018 ). Briefly, 7-day-old seedlings treated with Dex were pre-fixed in 2% glutaraldehyde in 0.1 M sodium phosphate buffer (pH 7.4) at room temperature for 2 h and at 4 °C overnight.…”

Section: Methodsmentioning

confidence: 99%

Expression of a fungal exo-β-1,3-galactanase in Arabidopsis reveals a role of type II arabinogalactans in the regulation of cell shape

Yoshimi

Hara

Yoshimura

et al. 2020

Journal of Experimental Botany

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Abstract Arabinogalactan-proteins (AGPs) are a family of plant extracellular proteoglycans implicated in many physiological events. AGP is decorated with type II arabinogalactans (AGs) consisting of a β-1,3-galactan backbone and β-1,6-galactan side chains, to which other sugars are attached. Based on the fact that a type II AG-specific inhibitor, β-Yariv reagent, perturbs growth and development, it has been proposed that type II AGs participate in the regulation of cell shape and tissue organization. However, the mechanisms by which type II AGs participate have not yet been established. Here, we describe a novel system that causes specific degradation of type II AGs in Arabidopsis, by which a gene encoding a fungal exo-β-1,3-galactanase that specifically hydrolyzes β-1,3-galactan backbones of type II AGs is expressed under the control of a dexamethasone-inducible promoter. Dexamethasone treatment increased the galactanase activity, leading to a decrease in Yariv reagent-reactive AGPs in transgenic Arabidopsis. We detected the typical oligosaccharides released from type II AGs by Il3GAL in the soluble fraction, demonstrating that Il3GAL acted on type II AG in the transgenic plants. Additionally, this resulted in severe tissue disorganization in the hypocotyl and cotyledons, suggesting that the degradation of type II AGs affected the regulation of cell shape.

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Section: Methodsmentioning

confidence: 99%

Expression of a fungal exo-β-1,3-galactanase in Arabidopsis reveals a role of type II arabinogalactans in the regulation of cell shape

Yoshimi

Hara

Yoshimura

et al. 2020

Journal of Experimental Botany

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“…The number of amino acids with increased abundance also increased in the “ rbohd/f _NaCl versus WT_NaCl” comparison group, relative to the “ rbohd/f _CK versus WT_CK” comparison group (Figure 4b ). Most of the metabolites with increased abundance were involved in “galactose metabolism” and “arginine and proline metabolism,” which contribute to the energy supply available for plant growth, as well as plant defense (Abedi et al, 2018 ; Majumdar et al, 2016 ) (Figure 3a,b ). In contrast, the number of pyrimidine and purine metabolites decreased in rbohd/f _NaCl samples in the “ rbohd/f _NaCl versus WT_NaCl” comparison group, including cGMP and UDP‐D‐galactose (Figure 4b ).…”

Section: Resultsmentioning

confidence: 99%

FLS2‐RBOHD module regulates changes in the metabolome of Arabidopsis in response to abiotic stress

Liu

Qin

et al. 2023

Plant Enviro Interactions

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Through crosstalk, FLAGELLIN SENSITIVE 2 (FLS2) and RESPIRATORY BURST OXIDASE HOMOLOG D (RBOHD) are involved in regulating the homeostasis of cellular reactive oxygen species (ROS) and are linked to the metabolic response of plants toward both biotic and abiotic stress. In the present study, we examined the metabolome of Arabidopsis seedlings under drought and salt conditions to better understand the potential role of FLS2 and RBOHD‐dependent signaling in the regulation of abiotic stress response. We identified common metabolites and genes that are regulated by FLS2 and RBOHD, and are involved in the response to drought and salt stress. Under drought conditions, D‐aspartic acid and the expression of associated genes, such as ASPARAGINE SYNTHASE 2 (ASN2), increased in both fls2 and robed/f double mutants. The accumulation of amino acids, carbohydrates, and hormones, such as L‐proline, D‐ribose, and indoleacetaldehyde increased in both fls2 and rbohd/f double mutants under salt conditions, as did the expression of related genes, such as PROLINE IMINOPEPTIDASE, PHOSPHORIBOSYL PYROPHOSPHATE SYNTHASE 5, and NITRILASE 3. Collectively, these results indicate that the FLS2‐RBOHD module regulates plant response to drought and salt stress through ROS signaling by adjusting the accumulation of metabolites and expression of genes related to metabolite synthesis.

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“…On chromosome 2A, the candidate genes linked with SNPs of QTL involved with Sev_Type_II_2019 were UDP-galactose transporter 1-like and ABC transporter C family member 2-like. The former, also known as hUGT1, increased the lignin content and hardness of leaves and stems in tobacco [50], whereas ABC transporters are involved in cuticular lipid secretion [51]. We believe that both the aforesaid genes provided Type II resistance against FHB in ITMI/MP to some degree by providing hardness to the plant tissue which restricted the entry of the pathogen and caused little damage.…”

Section: Discussionmentioning

confidence: 98%

Mapping Resistance to Argentinean Fusarium (Graminearum) Head Blight Isolates in Wheat

Sgarbi

Malbrán

Saldúa

et al. 2021

IJMS

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Fusarium head blight (FHB) of wheat, caused by Fusarium graminearum (Schwabe), is a destructive disease worldwide, reducing wheat yield and quality. To accelerate the improvement of scab tolerance in wheat, we assessed the International Triticeae Mapping Initiative mapping population (ITMI/MP) for Type I and II resistance against a wide population of Argentinean isolates of F. graminearum. We discovered a total of 27 additive QTLs on ten different (2A, 2D, 3B, 3D, 4B, 4D, 5A, 5B, 5D and 6D) wheat chromosomes for Type I and Type II resistances explaining a maximum of 15.99% variation. Another four and two QTLs for thousand kernel weight in control and for Type II resistance, respectively, involved five different chromosomes (1B, 2D, 6A, 6D and 7D). Furthermore, three, three and five QTLs for kernel weight per spike in control, for Type I resistance and for Type II resistance, correspondingly, involved ten chromosomes (2A, 2D, 3B, 4A, 5A, 5B, 6B, 7A, 7B, 7D). We were also able to detect five and two epistasis pairs of QTLs for Type I and Type II resistance, respectively, in addition to additive QTLs that evidenced that FHB resistance in wheat is controlled by a complex network of additive and epistasis QTLs.

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Expression of the human UDP-galactose transporter gene hUGT1 in tobacco plants' enhanced plant hardness

Cited by 6 publications

References 29 publications

Expression of a fungal exo-β-1,3-galactanase in Arabidopsis reveals a role of type II arabinogalactans in the regulation of cell shape

Expression of a fungal exo-β-1,3-galactanase in Arabidopsis reveals a role of type II arabinogalactans in the regulation of cell shape

FLS2‐RBOHD module regulates changes in the metabolome of Arabidopsis in response to abiotic stress

Mapping Resistance to Argentinean Fusarium (Graminearum) Head Blight Isolates in Wheat

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