Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013

Kenmoe, Sébastien; Vernet, Marie‐Astrid; Miszczak, Fabien; Dina, Julia; Schoenhals, Matthieu; Beng, Véronique Penlap; Vabret, Astrid; Njouom, Richard

doi:10.1186/s41182-018-0088-7

Cited by 16 publications

(13 citation statements)

References 49 publications

(60 reference statements)

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“…These results are similar to those observed in Central African Republic where a prevalence of 3.3% was noticed (24). But they are inferior to the results observed in Cameroon, Nigeria and Gabon where high prevalence rates of RSV were detected in febrile children with 7% in Cameroon (29), 17.7% in Nigeria (30) and 13.5% in Gabon (31). The study performed in Nigeria identified two subtypes of RSV, A and B, with a predominance of the subtype A.…”

Section: Discussioncontrasting

confidence: 79%

FilmArray Respiratory Panel Integrated in a field Point Of Care dispositive for the diagnosis of respiratory tract infections in rural areas in Senegal

Basséne¹,

Edouard

Diatta³

et al. 2018

Preprint

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17The development of molecular syndrome-based kits for the diagnosis of respiratory infections 18 offers rapid and sensitive detection of common respiratory pathogens and will have a 19 significant impact on the care of patients. In this study, we present the results obtained after 20 the introduction of the FilmArray respiratory panel in a field Point of care (POC) for the 21 diagnosis of virus and bacteria responsible of respiratory tract infections in Senegal rural area. 22 36 37

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Section: Discussioncontrasting

confidence: 79%

FilmArray Respiratory Panel Integrated in a field Point Of Care dispositive for the diagnosis of respiratory tract infections in rural areas in Senegal

Basséne¹,

Edouard

Diatta³

et al. 2018

Preprint

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“…Our study showed an overall prevalence of RSV of 8.0% in the period of 2015 to 2018, with significant annual (6.4%-10.6%) and seasonal (12.7% in rainy season vs 3.0% in dry season) fluctuations. While RSV circulation is high during the winter in temperate climates, 12 a high RSV incidence usually coincides with the rainy season in African countries with a tropical climate, such as in Kenya, 45 Cameroon, 46 Senegal, 34 and Ghana. 47 Also in CAF, most cases were reported in November in a study from 2010.…”

Section: Discussionmentioning

confidence: 99%

Epidemiology and genetic characterization of respiratory syncytial virus in children with acute respiratory infections: Findings from the influenza sentinel surveillance network in Central African Republic, 2015 to 2018

Komoyo

Yambiyo

Manirakiza

et al. 2021

Health Science Reports

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Background and aims Respiratory syncytial virus (RSV) is one of the main viral pathogens causing acute respiratory infections in children under 5 years of age but has seldom been studied in Central African Republic (CAF). Taking advantage of the national influenza surveillance network in CAF, this study aimed at providing the first insights into RSV prevalence and seasonality over 4 years of surveillance and the clinical manifestations of RSV in this population in CAF. Methods A total of 3903 children under 5 years matching the influenza‐like illness (ILI, 68.5%) or severe acute respiratory infection (SARI, 31.5%) case definitions were recruited from January 2015 to December 2018. The presence of RSV viral RNA in nasopharyngeal samples was assessed by RT‐PCR, followed by RSV‐A and RSV‐B typing and Sanger sequencing on a subset of samples. Phylogenetic analyses were carried on partial G protein sequences. Associations between RSV and demographic or clinical manifestations were investigated by statistical analyses. Results RSV prevalence was significantly higher in infants <6 months (13.4%), in hospitalized children (13.3% vs 5.5%) and in male patients (9.5% vs 6.4%). An overall prevalence of RSV of 8.0% in the period of 2015 to 2018 was shown, with significant annual (6.4%‐10.6%) and seasonal (12.7% in rainy season vs 3.0% in dry season) fluctuations. While RSV seasons in 2015, 2016, and 2018 were relatively similar, 2017 showed deviations from the overall patterns with significantly higher RSV circulation and an outbreak peak 3 to 5 months earlier. Concomitant circulation of RSV‐A and RSV‐B with an alternating predominance of RSV‐A and RSV‐B strains and temporal RSV‐A genotype replacement from NA1 to ON1 was observed. Conclusion This study represents the first in‐depth epidemiological analysis of RSV in CAF and provides first insights into RSV genetic diversity and seasonality in the country.

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“…Nasopharyngeal swabs were screened to detect respiratory viruses using r-gene commercial duplex AdV/HBoV r-gene™ (Respiratory Multi Well System r-gene™, BioMerieux, Lyon, France) as described elsewhere [ 14 ]. A total of 220 HAdV positive samples were used for further characterization by PCR carried out on hexon gene.…”

Section: Methodsmentioning

confidence: 99%

Molecular characterization of human adenovirus associated with acute respiratory infections in Cameroon from 2011 to 2014

Kenmoe

Vernet

Goff

et al. 2018

Virol J

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BackgroundHuman adenoviruses (HAdVs) cause a wide range of diseases worldwide, including respiratory infections. Studies on HAdV molecular epidemiology are limited in Cameroon. The purpose of this study is to document the different types HAdV circulating in Cameroon in children with acute respiratory infections.MethodsNasopharyngeal swabs were collected from 811 children under 15 years from 2011 to 2014. The HAdV detection was assessed by semi-quantitative generic PCR r-gene®. The HAdV-positive samples were typed by amplification and sequencing of partial hexon gene and a real-time PCR. Demographic data were collected and analyzed. The infection and hospitalization risk factors were assessed thought the Chi-square test.ResultsA total of 137/220 HAdV-positive samples were amplified successfully. Six species of HAdV (Mastadenovirus A to F) were detected with B (108/220) and C (47/220) being the predominant strains. Hospitalization and age were significantly associated to HAdV-B and HAdV-C respectively. Phylogenetic analysis of HAdV-B3 virus (18) and B7 (5) shows a conserved and a significant temporal stability in relation to the reference sequence (99.1 to 100% of similarity).ConclusionThis study reported HAdV species and types detected in children with acute respiratory infections in Cameroon between September 2011 and July 2014. These results support further evaluation of the spatio-temporal circulation pattern of HAdV species and types in Cameroon.

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Genetic diversity of human respiratory syncytial virus isolated among children with acute respiratory infections in Southern Cameroon during three consecutive epidemic seasons, 2011–2013

Cited by 16 publications

References 49 publications

FilmArray Respiratory Panel Integrated in a field Point Of Care dispositive for the diagnosis of respiratory tract infections in rural areas in Senegal

FilmArray Respiratory Panel Integrated in a field Point Of Care dispositive for the diagnosis of respiratory tract infections in rural areas in Senegal

Epidemiology and genetic characterization of respiratory syncytial virus in children with acute respiratory infections: Findings from the influenza sentinel surveillance network in Central African Republic, 2015 to 2018

Molecular characterization of human adenovirus associated with acute respiratory infections in Cameroon from 2011 to 2014

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