Gene expression profiling in healthy newborns from diverse localities of the Czech Republic

Abstract

Prenatal exposure to air pollution is associated with intrauterine growth restriction and low birth weight. Gene expression changes in newborns in relation to air pollution have not been sufficiently studied. We analyzed whole genome expression in cord blood leukocytes of 202 newborns from diverse localities of the Czech Republic, differing among other factors in levels of air pollution: the district of Karvina (characterized by higher concentration of air pollutants) and Ceske Budejovice (lower air pollution levels). We aimed to identify differentially expressed genes (DEGs) and pathways in relation to locality and concentration of air pollutants. We applied the linear model to identify the specific DEGs and the correlation analysis, to investigate the relationship between the concentrations of air pollutants and gene expression data. An analysis of biochemical pathways and gene set enrichment was also performed. In general, we observed modest changes of gene expression, mostly attributed to the effect of the locality. The highest number of DEGs was found in samples from the district of Karvina. A pathway analysis revealed a deregulation of processes associated with cell growth, apoptosis or cellular homeostasis, immune response-related processes or oxidative stress response. The association between concentrations of air pollutants and gene expression changes was weak, particularly for samples collected in Karvina. In summary, as we did not find a direct effect of exposure to air pollutants, we assume that the general differences in the environment, rather than actual concentrations of individual pollutants, represent a key factor affecting gene expression changes at delivery. Environ. Mol. Mutagen. 59:401-415, 2018. © 2018 Wiley Periodicals, Inc.

Benzo[a]pyrene-associated Oxidative Stress is Divergent in Non-atopic Versus Atopic Children, and Suggest They May Represent Asthma Endotypes: A Case-control Study

“…In our investigation, we could not directly examine whether non-atopic asthma represents T-helper 2 or type 2 low asthma, while atopic asthma captures Thelper 2 or type 2 high asthma, two known endotypes [3,30]. This is because we did not measure typical [9,10,13,19,[31][32][33][34][35][36]. Both the laboratory and epidemiologic evidence have shown that PAHs could induce or enhance allergic sensitization, exacerbate pre-existing asthma, and enhance the risk of de novo asthma development [33,37,38].…”

“…cigarette smoke and other anthropogenic compounds) have been shown to predict de novo asthma development during childhood [8]. Our group too has documented signi cant associations among neighborhood level of polyaromatic hydrocarbon (PAH), speci cally, benzo[a]pyrene (B[a]P), multiple putative biomarkers, and clinically diagnosed asthma in children [9][10][11][12][13][14][15]. Furthermore, our analysis suggest for the rst time that childhood B[a]P exposure predicts dose-responsive, integrative -Omics based mechanisms of asthma, mediated by dysregulated hematopoiesis among the asthma cases, compared to the controls [11].…”

Impact of Air Pollution on the Health of the Population in Parts of the Czech Republic

“…It significantly increased in newborns in Karvina. DNA adducts [ 52 ], oxidative damage (DNA oxidation, lipid peroxidation) [ 53 ], and whole genome expression revealed a deregulation of processes associated with immune response or oxidative stress response [ 54 ]. In the urine of newborns, monohydroxylated metabolites of PAHs (OH-PAHs) were analyzed.…”

Influences the Aeromath in the Way of Ending Births

“…Honkova et al [40] analyzed whole genome expression in cord blood of leukocytes of 202 newborns from the districts of Karvina and Ceske Budejovice. They aimed to identify differentially expressed genes and pathways in relation to locality and concentration of air pollutants.…”