Development and validation of a real-time PCR specific for the leukotoxin gene of <i>Bibersteinia trehalosi</i>

Killion, Halcyon J.; Jennings-Gaines, Jessica E.; Fox, Karen A.; Sondgeroth, Kerry S.

doi:10.1177/1040638717753497

Cited by 5 publications

(2 citation statements)

References 16 publications

(19 reference statements)

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“…The amino acid sequence of the structural toxin LktA was identical for the three M. pernigra strains, but <85 % similar to any other LktA within the genus, indicating a new member of the RTX toxin family [19]. The lktA gene might serve as template for developing a species-specific diagnostic PCR for M. pernigra [20].…”

Section: Full-textmentioning

confidence: 99%

Mannheimia pernigra sp. nov., isolated from bovine respiratory tract

Kuhnert

Brodard

Schönecker

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Over a period of 1 year, 270 isolates identified as Taxon 39 of Bisgaard were obtained from the nasopharynx of veal calves at 11 epidemiologically independent Swiss fattening farms. Two isolates from each farm and the Australian Taxon 39 reference strain BNO311 were further characterized by genetic and phenotypic methods. Phylogenetic analysis of 16S rRNA and recN gene sequences placed the isolates in a single, distinct cluster within the genus Mannheimia . As to the rpoB gene, most isolates clustered together, but four strains formed a separate cluster close to Mannheimia varigena . Genome sequence analysis of isolates from both rpoB clusters confirmed their species status, with an average nucleotide identity (ANI) >98.9 % between isolates and <84 % to the closest species, M. varigena . Based upon whole genome sequences, the G+C content was determined as 39.1 mol%. Similarly, analysis of MALDI-TOF MS reference spectra clustered the isolates clearly separated from the other Mannheimia species, making this the method of choice for identification. In addition, numerous biochemical markers based on classical as well as commercial identification schemes were determined, allowing separation from other Mannheimia species and identification of the new taxon. Major fatty acids for strain 17CN0883T are C14 : 0, C16 : 0, C16 : 1 ω7c and C18 : 1 ω7c. Major respiratory quinones are ubiquinone-7 and ubiquinone-8. We propose the name Mannheimia pernigra sp. nov. for former Taxon 39 of Bisgaard. The type strain is 17CN0883T (=CCUG 74657T=DSM 111153T) isolated from a veal calf in Switzerland.

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Section: Full-textmentioning

confidence: 99%

Mannheimia pernigra sp. nov., isolated from bovine respiratory tract

Kuhnert

Brodard

Schönecker

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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“…Several virulence factors have been identified in B. trehalosi, including fimbriae, polysaccharide capsule, and lipopolysaccharide (5), as well as a leukotoxin, which is considered to be a major virulence factor (6, 7). In addition to virulence factors, increasing antimicrobial resistance has been reported in bacterial respiratory pathogens (8).…”

Section: Announcementmentioning

confidence: 99%

Whole-Genome Sequence of Multidrug-Resistant Bibersteinia trehalosi Strain OADDL-BT1

Narayanan¹,

Bates²,

Confer³

et al. 2019

Microbiol Resour Announc

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Bibersteinia

Blackall¹

2020

Bergey's Manual of Systematics of Archaea and Bacteria

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Bi.ber.stei'ni.a. N.L. fem. n . Bibersteinia bacterial genus named after Ernst L. Biberstein, who did much of the early characterization work on this organism, including the creation of the serotyping scheme and some of the earliest DNA–DNA relatedness studies that indicated the unique nature of this taxon. Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae / Bibersteinia Bibersteina is a genus within the family Pasteurellaceae . Members of this genus are Gram‐negative, nonmotile, rod‐shaped, or pleomorphic cells occurring singly and in pairs or short chains. Most isolates are hemolytic on sheep blood agar and are CAMP‐positive. Growth is mesophilic and facultatively anaerobic or microaerophilic. Nitrate is reduced without gas production. The reaction in Hugh–Leifson medium with (+)‐ d ‐glucose is fermentative without gas production. Variable reactions have been reported for the catalase and oxidase tests. Members of this genus have been associated with systemic infections in lambs and adult sheep and pneumonia in domestic sheep. The role of the organism as a cause of disease in cattle and in wild sheep, particularly bighorn sheep ( Ovis canadensis ), is controversial with reports of the organism being part of the normal flora and other reports linking the organism to disease. DNA G + C content (mol%) : 42.6% ( T m ). Type species : Bibersteinia trehalosi Blackall et al. 2007 VP .

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Development and validation of a real-time PCR specific for the leukotoxin gene of Bibersteinia trehalosi

Cited by 5 publications

References 16 publications

Mannheimia pernigra sp. nov., isolated from bovine respiratory tract

Mannheimia pernigra sp. nov., isolated from bovine respiratory tract

Whole-Genome Sequence of Multidrug-Resistant Bibersteinia trehalosi Strain OADDL-BT1

Bibersteinia

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