Whole-Genome Sequence of Multidrug-Resistant Bibersteinia trehalosi Strain OADDL-BT1

Narayanan, Sai; Bates, Haley; Confer, Anthony W.; Couger, Brian; Ramachandran, Akhilesh

doi:10.1128/mra.01690-18

Cited by 3 publications

(1 citation statement)

References 15 publications

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“…H. somni , P. multocida , and B. trehalosi strains isolated from clinical cases that had been subjected to MIC testing were also used for evaluation purposes along with ATCC reference strains ( Table S4 ). Bibersteinia trehalosi was included in the reference set because it is occasionally isolated from BRD cases and has shown AMR [ 26 ]. This collection of reference strains was employed only for evaluating assay performance and specificity and was excluded from further data analysis of the clinical samples.…”

Section: Methodsmentioning

confidence: 99%

Development of a Multiplex Real-Time PCR Assay for Predicting Macrolide and Tetracycline Resistance Associated with Bacterial Pathogens of Bovine Respiratory Disease

et al. 2021

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Antimicrobial resistance (AMR) in bovine respiratory disease (BRD) is an emerging concern that may threaten both animal and public health. Rapid and accurate detection of AMR is essential for prudent drug therapy selection during BRD outbreaks. This study aimed to develop a multiplex quantitative real-time polymerase chain reaction assay (qPCR) to provide culture-independent information regarding the phenotypic AMR status of BRD cases and an alternative to the gold-standard, culture-dependent test. Bovine clinical samples (297 lung and 111 nasal) collected in Nebraska were subjected to qPCR quantification of macrolide (MAC) and tetracycline (TET) resistance genes and gold-standard determinations of AMR of BRD pathogens. Receiver operating characteristic curve analysis was used to classify AMR based on the qPCR results. For lung tissues, the qPCR method showed good agreement with the gold-standard test for both MACs and TETs, with a sensitivity of 67–81% and a specificity higher than 80%. For nasal swabs, qPCR results passed validation criteria only for TET resistance detection, with a sensitivity of 88%, a specificity of 80% and moderate agreement. The culture-independent assay developed here provides the potential for more rapid AMR characterization of BRD cases directly from clinical samples at equivalent accuracy and higher time efficiency compared with the gold-standard, culture-based test.

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Section: Methodsmentioning

confidence: 99%

Development of a Multiplex Real-Time PCR Assay for Predicting Macrolide and Tetracycline Resistance Associated with Bacterial Pathogens of Bovine Respiratory Disease

et al. 2021

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Bibersteinia

Blackall¹

2020

Bergey's Manual of Systematics of Archaea and Bacteria

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Bi.ber.stei'ni.a. N.L. fem. n . Bibersteinia bacterial genus named after Ernst L. Biberstein, who did much of the early characterization work on this organism, including the creation of the serotyping scheme and some of the earliest DNA–DNA relatedness studies that indicated the unique nature of this taxon. Proteobacteria / Gammaproteobacteria / Pasteurellales / Pasteurellaceae / Bibersteinia Bibersteina is a genus within the family Pasteurellaceae . Members of this genus are Gram‐negative, nonmotile, rod‐shaped, or pleomorphic cells occurring singly and in pairs or short chains. Most isolates are hemolytic on sheep blood agar and are CAMP‐positive. Growth is mesophilic and facultatively anaerobic or microaerophilic. Nitrate is reduced without gas production. The reaction in Hugh–Leifson medium with (+)‐ d ‐glucose is fermentative without gas production. Variable reactions have been reported for the catalase and oxidase tests. Members of this genus have been associated with systemic infections in lambs and adult sheep and pneumonia in domestic sheep. The role of the organism as a cause of disease in cattle and in wild sheep, particularly bighorn sheep ( Ovis canadensis ), is controversial with reports of the organism being part of the normal flora and other reports linking the organism to disease. DNA G + C content (mol%) : 42.6% ( T m ). Type species : Bibersteinia trehalosi Blackall et al. 2007 VP .

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Bovine Respiratory Disease: Conventional to Culture-Independent Approaches to Studying Antimicrobial Resistance in North America

et al. 2022

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Numerous antimicrobial resistance (AMR) surveillance studies have been conducted in North American feedlot cattle to investigate the major bacterial pathogens of the bovine respiratory disease (BRD) complex, specifically: Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, and Mycoplasma bovis. While most bacterial isolates recovered from healthy cattle are susceptible to a repertoire of antimicrobials, multidrug resistance is common in isolates recovered from cattle suffering from BRD. Integrative and conjugative elements (ICE) have gained increasing notoriety in BRD-Pasteurellaceae as they appear to play a key role in the concentration and dissemination of antimicrobial resistant genes. Likewise, low macrolide susceptibility has been described in feedlot isolates of M. bovis. Horizontal gene transfer has also been implicated in the spread of AMR within mycoplasmas, and in-vitro experiments have shown that exposure to antimicrobials can generate high levels of resistance in mycoplasmas via a single conjugative event. Consequently, antimicrobial use (AMU) could be accelerating AMR horizontal transfer within all members of the bacterial BRD complex. While metagenomics has been applied to the study of AMR in the microbiota of the respiratory tract, the potential role of the respiratory tract microbiome as an AMR reservoir remains uncertain. Current and prospective molecular tools to survey and characterize AMR need to be adapted as point-of-care technologies to enhance prudent AMU in the beef industry.

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Whole-Genome Sequence of Multidrug-Resistant Bibersteinia trehalosi Strain OADDL-BT1

Abstract: The genome of a multidrug-resistant strain of Bibersteinia trehalosi isolated from a calf with chronic pneumonia is presented. The draft genome sequences have been deposited at DDBJ/ENA/GenBank.

Cited by 3 publications

References 15 publications

Development of a Multiplex Real-Time PCR Assay for Predicting Macrolide and Tetracycline Resistance Associated with Bacterial Pathogens of Bovine Respiratory Disease

Development of a Multiplex Real-Time PCR Assay for Predicting Macrolide and Tetracycline Resistance Associated with Bacterial Pathogens of Bovine Respiratory Disease

Bibersteinia

Bovine Respiratory Disease: Conventional to Culture-Independent Approaches to Studying Antimicrobial Resistance in North America

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