Layer‐by‐layer buildup of polysaccharide‐containing films: Physico‐chemical properties and mesenchymal stem cells adhesion

Abstract

Layer-by-Layer assembled polyelectrolyte films offer the opportunity to control cell attachment and behavior on solid surfaces. In the present study, multilayer films based on negatively charged biopolymers (pectin, dextran sulfate, carboxymethylcellulose) and positively charged polysaccharide chitosan or synthetic polyelectrolyte polyethyleneimine has been prepared and evaluated. Physico-chemical properties of the formed multilayer films, including their growth, morphology, wettability, stability, and mechanical properties, have been studied. We demonstrated that chitosan-containing films are characterized by the linear growth, the defect-free surface, and predominantly viscoelastic properties. When chitosan is substituted for the polyethyleneimine in the multilayer system, the properties of the formed films are significantly altered: the rigidity and surface roughness increases, the film growth acquires the exponential character. The multilayer films were subsequently used for culturing mesenchymal stem cells. It has been determined that stem cells effectively adhered to chitosan-containing films and formed on them the monolayer culture of fibroblast-like cells with high viability. Our results show that cell attachment is a complex process which is not only governed by the surface functionality because one of the key parameter effects on cell adhesion is the stiffness of polyelectrolyte multilayer films. We therefore propose our Layer-by-Layer films for applications in tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2093-2104, 2018.

Polyvinyl alcohol and pectin blended films: Preparation, characterization, and mesenchymal stem cells attachment

“…It could be assumed that such cell behavior can be dealt with mechanical properties of film surface or toxic effect of residual GA on MSCs. Many researchers have previously shown that one of the key parameter effects on MSCs adhesion is the stiffness of film surface 12,13,49‐51 . The elastic properties of film surface can be quantitatively evaluated by PeakForce QNM (force volume mode).…”

“…Isolation of MSCs was performed by enzymatic treatment of Wistar rat fat homogenate in accordance with the previously reported procedure 13 . Briefly, after treatment of fat in the 0.1% collagenase solution (30 min, 37°C) the cell suspension was filtered through a capron filter (100 μm) and centrifuged at 370 × g for 10 min.…”

“…Pectin‐based materials have been proposed for tissue engineering, wound dressing, drug delivery, and other biomedical applications 11,12 . It has been previously shown that 3D porous scaffolds and ultrathin films from pectin were favorable for effective adhesion of viable MSCs 13‐15 . Pectin‐based biomaterials have also been proposed to use as novel excellent wound healing dressings 16,17 .…”

Study on the stability and cellular affinity of gelatin‐polysaccharide composite films

“…Despite the numerous works report the effect of polysaccharide addition on mechanical and biological properties of gelatin films, however, information about the stability and cellular affinity in high humidity environment is still limited. The improvement of stability and cellular affinity is important to maintain the integrality of films acting as skin barriers and support the repair process of stem cells (Afewerki et al, 2018; Dash et al, 2018; Kulikouskaya et al, 2018). This study prepared two kinds of gelatin‐polysaccharide composite films, including gelatin‐HA (G‐HA) and gelatin‐CS (G‐CS), and investigated the differences in the stability and cellular affinity between these two composite films.…”

Application of bioderadable materials based on alginate and pectin to prevent the formation of peritoneal adhesions

“…При клеточной трансплантации отмечены сходные с альгинатами свойства [19]. Кроме того, тонкие пленки на основе пектина пригодны для эффективной адгезии МСК [20]. Композиционные полимер-неорганические материалы на основе пектинов, например нанокомпозиты пектин-Ag, проявляют собственную антибактериальную активность и обеспечивают синергетический антибактериальный эффект в сочетании с аминогликозидными антибиотиками [20].…”