Polyvinyl alcohol and pectin blended films: Preparation, characterization, and mesenchymal stem cells attachment

Abstract

The design of novel wound dressings for chronic wound treatment is still of great importance. One of the promising approaches is application of mesenchymal stem cells (MSCs), immobilized on a flexible polymer film, for healing. In this study, blended films based on polyvinyl alcohol (PVA) and pectin with different component ratio have been prepared by solution casting method and evaluated. Physicochemical properties of the formed PVA/pectin films, including their morphology, wettability, swelling, stability, mechanical characteristics, have been studied. We demonstrated that the surface of PVA/pectin films could be modified by ultraviolet or dielectric barrier discharge plasma exposure. After both ultraviolet and plasma treatment, the hydrophilicity of PVA/pectin films increased. It has been shown that additional crosslinking of PVA/pectin films with glutaraldehyde resulted in reinforcement of their structure. MSCs were cultured on neat and modified PVA/pectin samples to evaluate the effects of film characteristics and composition on cell behavior. It has been determined that MSCs effectively adhered to glutaraldehyde‐crosslinked PVA/pectin films and formed on them the monolayer culture of fibroblast‐like cells. The additional modification of PVA/pectin films with collagen resulted in enhancement of MSCs adhesion. Our results show that the obtained PVA/pectin films with adhered MSCs can be suggested for potential application as a part of novel complex wound dressings.

Using biomaterials to improve mesenchymal stem cell therapies for chronic, nonhealing wounds

“…Polymeric biomaterials can take the form of scaffolds, 48,49 films, 50 hydrocolloids, 51 hydrogels, [52][53][54][55][56][57] hydrofibers, 58 and electrospun nanofibers. 59 Among these, hydrogels are widely preferred due to their superior properties, such as biodegradability, 60 high water content retention, 60,61 easy fabrication, 62 high capacity for drug loading, 63 immunomodulation, 64 and their ability to boost MSC immunomodulatory properties.…”

Pectin-Based Scaffolds for Tissue Engineering Applications

“…Pectin-based patches produced with this approach support cell adhesion and proliferation and accelerate the processes occurring during the formation of new tissue [27][28][29][30]. Moreover, films with high toughness and stretchability can be produced with solvent casting, and these can be potentially used as pectin-based patches for load-bearing tissues (e.g., cartilage, tendon) [28].…”

Development and characterization of antibacterial packaging film based on poly (vinyl alcohol)/agarose enriched with cinnamon oil

“…Nevertheless, the strong affinity toward water molecules has limited the utilization of PVOH in this area because the hydrophilic surface of PVOH could facilitate to the development of foodborne pathogens and changes in food quality during storage and distribution. With a goal to enhance such drawbacks and enrich the functionalities, blending PVOH with other biopolymers such as starch (Tian et al, 2017), gelatin (Pawde et al, 2008), pectin (Kraskouski et al, 2021), chitosan (Liu et al, 2018), and agarose (Bui et al, 2021) was effectively performed. In above‐mentioned biopolymers, agarose is preferred as it is a polysaccharide isolated from seaweed comprised of repetition of d ‐galactose and 3,6‐anhydro‐ l ‐galactose (Carina et al, 2021).…”