In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231

Nordin, Muhammad Luqman; Kadir, Arifah Abdul; Zakaria, Zainul Amiruddin; Abdullah, Rasedee; Abdullah, M.

doi:10.1186/s12906-018-2153-5

Cited by 51 publications

(44 citation statements)

References 40 publications

(39 reference statements)

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“…This means that polar solvent was used since methanol and water were classified as polar solvent. Hydromethanolic extract (mixture of methanol and water) has greater efficiency in pulling out (extract) phytochemical compound in plant compared to pure solvent (without mixture) [20,26,27]. Interestingly, in this study, it was found that both antibacterial and cytotoxic activities of this plant are considered weak.…”

Section: Discussionmentioning

confidence: 82%

“…[19] and Nordin et al . [20]. Cytotoxicity activity of S. polyanthum extract was prepared with concentrations ranging from 15.63 µg/mL to 1000 µg/mL.…”

Section: Methodsmentioning

confidence: 99%

“…The cytotoxic effect of S. polyanthum extract against 4T1 was recorded as IC 50 and compared with untreated cells according to the method described by Nordin et al . [20] and Ayob et al . [21].…”

Section: Methodsmentioning

confidence: 99%

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Antibacterial and cytotoxic activities of the Syzygium polyanthum leaf extract from Malaysia

et al. 2019

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Background and Aim: The increasing prevalence of drug resistance eventually leads scientist to discover new drugs that could solve the problem. Since ancient immemorial times, medicinal plants generally known as herbs were widely used in every culture throughout the world. In fact, currently up to 70,000 plant species have been screened for biological activities and about 70% ends up for commercialization. Therefore, this study was aimed to evaluate the potential cytotoxic and antibacterial effect of Syzygium polyanthum leaves which are local Malaysia plants, against 4T1 and MCF-7 mammary carcinoma cells, respectively, and also against bacteria causing mastitis in cows. Materials and Methods: The cytotoxic effect of hydromethanolic extract of S. polyanthum against 4T1 and MCF-7 mammary carcinoma cells was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The cells were treated with the concentration of extracts ranging from 15.63 μg/mL to 1000 μg/ml for 72 h, and the percentage of cell survivability was determined based on minimum concentration that was able to allow at least 50% growth of cancer cells (IC50) after 72 h. The antibacterial activity was tested against common bacteria causing mastitis in cow. The bacteria were isolated from milk samples. The antibacterial activity of the extract was determined by disk diffusion method and susceptibility test based on minimum inhibitory concentration (MIC). Results: Staphylococcus aureus, Staphylococcus hyicus, and Staphylococcus intermedius were isolated from the milk samples that positive for mastitis. The MIC values range from 7.12 mm to 13.5 mm. The extract exhibits the widest zone of inhibition (13.5±0.20 mm) at 1000 mg/ml of concentrations. The extract relatively has low cytotoxicity effect against 4T1 and MCF-7 cells with IC50 values ranging from 672.57±59.42 and 126.05±50.89 μg/ml, respectively. Conclusion: S. polyanthum exerts weak antibacterial activity and cytotoxic effect to mammary carcinoma cells. The extract does not toxic to cells. However, further study is recommended, especially, this plant should be tested for in vivo.

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Section: Discussionmentioning

confidence: 82%

“…[19] and Nordin et al . [20]. Cytotoxicity activity of S. polyanthum extract was prepared with concentrations ranging from 15.63 µg/mL to 1000 µg/mL.…”

Section: Methodsmentioning

confidence: 99%

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Antibacterial and cytotoxic activities of the Syzygium polyanthum leaf extract from Malaysia

et al. 2019

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“…The extracting ratio of total flavonoids from T. jasminoides by an ultrasonic wave cooperated method was 3.22% [28], which was a bit higher than that achieved by our ethanol soaking extraction. It is commonly considered that medicinal plants with high amounts of phenols and flavonoids have potential antioxidant actions [29,30], which prompted us to evaluate the antioxidant activity.…”

Section: Total Phenolic and Flavonoid Contentmentioning

confidence: 99%

Cytotoxic activities against MCF-7 and MDA-MB-231, antioxidant andα-glucosidase inhibitory activities ofTrachelospermum jasminoidesextractsin vitro

Song

Zhang

Gong

et al. 2019

Biotechnology & Biotechnological Equipment

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The present study investigated the cytotoxic activities against MCF-7 and MDA-MB-231 cells, and the potential antioxidant and a-glucosidase inhibitory activities of the extracts of the aerial parts of Trachelospermum jasminoides, a traditional medicinal plant used for the treatment of rheumatic arthralgia in China. In MTT assay and microscopic analysis, the ethyl acetate extract (EAE) of T. jasminoides showed moderate cytotoxic effect against MCF-7 and MDA-MB-231 with IC 50 values of 109.54 ± 2.39 lg/mL and 162.68 ± 9.52 lg/mL, respectively, but the water extract (WE) was inactive against both of them. With gallic acid and rutine as equivalents for determination, the total phenolic and total flavonoid contents in the EAE (54.93 ± 0.32 mg GAE/g and 186.75 ± 4.55 mg RE/ g, respectively) were higher than those of the WE. The antioxidant activities of the EAE and WE, which were evaluated by reducing power assay, DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging assay and hydroxyl radical scavenging assay, may be directly associated with the high contents of phenols and flavonoids in T. jasminoides. The EAE showed higher inhibitory effect in the a-glucosidase inhibition tests with an IC 50 value of 776.81 ± 107.80 lg/mL as compared to the WE with 2607.48 ± 364.31 lg/mL. Furthermore, the antioxidant activity and the inhibitory effects of the fractions against a-glucosidase were concentration-dependent. ARTICLE HISTORY

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“…About 500 species of evergreen trees and shrubs are commenced throughout the tropical and sub-tropical regions of the universe [8]. Besides, A. compressa possesses significant hypoglycemic effects in Type 2 diabetic rats, and antioxidant activity and A. crispa showed in vitro cytotoxic activity and antioxidative actions against breast cancer cell lines [9]. Barks and leaves of A. colorata were extracted with ethanol having antimicrobial activities [10].…”

Section: Introductionmentioning

confidence: 99%

In-vivo and in-vitro evaluation of pharmacological activities of Ardisia solanacea leaf extract

et al. 2019

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Background: The Bangladeshi rural and hilly areas people have long tradition to use various medicinal plants for treating different diseases. That's why, the crude methanolic leaf extract of Ardisia solanacea with its different fractions (petroleum ether, carbon tetrachloride, n-hexane and chloroform fractions) were subjected to investigate bioactivities in swiss albino mice; namely analgesic, CNS, and Oral hypoglycemic activities, while in-vitro evaluation of cytotoxicity. Methods: Central nervous system activity was investigated by various method such as Elevated plus maze, Hole board, Hole cross and Open field test apparatus. Analgesic activity was evaluated by both acetic acid induced and tail immersion method. Hypoglycemic activity was evaluated by oral glucose tolerance test and cytotoxicity was evaluated by Brine shrimp lethality bioassay. Results: In CNS activity, among others fractions, ASCF fraction produced a significant anxiolytic activity in both elevated plus maze and Hole board test. During open-field test almost all the fractions of A. solanacea leaves extract display decreased locomotor activities that indicates significant sedative activity. The ASME and ASCF showed significant peripheral analgesic activity at a dose of 200 mg/kg and 400 mg/kg body weight (p < 0.05). In tail immersion method, among others extracts chloroform fractions exhibited significant (p < 0.05) elongation of reaction time 30 min after oral dose of 200 and 400 mg/kg body weight respectively. The methanolic and n-hexane extracts reduced blood glucose level significantly after 90 min with value of 53.94% and 48.15% respectively (p < 0.05). In case of cytotoxicity activity, among other fractions carbon tetrachloride fraction showed lowest LC 50 values. Conclusions: From the above results, it is clear that different fractions of A. solanacea showed significant pharmacological potentiality in different in-vitro and in-vivo study model. So, it will be very much possible source for an isolating lead compound for curing the numerous disorders.

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In vitro investigation of cytotoxic and antioxidative activities of Ardisia crispa against breast cancer cell lines, MCF-7 and MDA-MB-231

Cited by 51 publications

References 40 publications

Antibacterial and cytotoxic activities of the Syzygium polyanthum leaf extract from Malaysia

Antibacterial and cytotoxic activities of the Syzygium polyanthum leaf extract from Malaysia

Cytotoxic activities against MCF-7 and MDA-MB-231, antioxidant andα-glucosidase inhibitory activities ofTrachelospermum jasminoidesextractsin vitro

In-vivo and in-vitro evaluation of pharmacological activities of Ardisia solanacea leaf extract

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