Genome sequence of an aflatoxigenic pathogen of Argentinian peanut, Aspergillus arachidicola

Abstract

BackgroundAspergillus arachidicola is an aflatoxigenic fungal species, first isolated from the leaves of a wild peanut species native to Argentina. It has since been reported in maize, Brazil nut and human sputum samples. This aflatoxigenic species is capable of secreting both B and G aflatoxins, similar to A. parasiticus and A. nomius. It has other characteristics that may result in its misidentification as one of several other section Flavi species. This study offers a preliminary analysis of the A. arachidicola genome.ResultsIn this study we sequenced the genome of the A. arachidicola type strain (CBS 117610) and found its genome size to be 38.9 Mb, and its number of predicted genes to be 12,091, which are values comparable to those in other sequenced Aspergilli. A comparison of 57 known Aspergillus secondary metabolite gene clusters, among closely-related aflatoxigenic species, revealed nearly half were predicted to exist in the type strain of A. arachidicola. Of its predicted genes, 691 were identified as unique to the species and 60% were assigned Gene Ontology terms using BLAST2GO. Phylogenomic inference shows CBS 117610 sharing a most recent common ancestor with A. parasiticus. Finally, BLAST query of A. flavus mating-type idiomorph sequences to this strain revealed the presence of a single mating-type (MAT1–1) idiomorph.ConclusionsBased on A. arachidicola morphological, genetic and chemotype similarities with A. flavus and A. parasiticus, sequencing the genome of A. arachidicola will contribute to our understanding of the evolutionary relatedness among aflatoxigenic fungi.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-4576-2) contains supplementary material, which is available to authorized users.

A comparative genomics study of 23 Aspergillus species from section Flavi

“…1b). Two of these (A. nomius and A. arachidicola 18,19 ) were also published by other groups in parallel to this work. We compare these 19 to previously sequenced section Flavi species (A. oryzae, A. flavus, A. sojae, and Fig.…”

Genome‐wide nucleotide variation distinguishesAspergillus flavusfromAspergillus oryzaeand helps to reveal origins of atoxigenicA. flavusbiocontrol strains

“…ATCC 12892 also had an averaged total SNPs 10‐fold greater than any other comparison between two A. oryzae isolates (Figs and S2). By cross analyses using species in Aspergillus section Flavi , ATCC 12982 was found to be more closely related to A. parasiticus (total SNPs count between them was approximately 500 000), distantly related to A. arachidicola (1 100 000) (Moore et al ) and even more distantly related to A. flavus/A. oryzae (1 850 000).…”

Genome Sequencing of Cladobotryum protrusum Provides Insights into the Evolution and Pathogenic Mechanisms of the Cobweb Disease Pathogen on Cultivated Mushroom

“…The terpene gene cluster in C . protrusum encodes 4,4′-piperazine-2,5-diyldimethyl-bis-phenol, which has high homology to Aspergillus flavus and has possible pharmacological properties [ 102 ]. Its associated hybrids encode mycotoxins (nivalenol/deoxynivalenol/3-acetyldeoxynivalenol and trichothecene) and phytotoxins (betaenone (A, B and C)).…”