LC-MS/MS method for the determination of the prodrug aripiprazole lauroxil and its three metabolites in plasma and its application to in vitro biotransformation and animal pharmacokinetic studies

A systematized and chemometrics‐assisted liquid chromatography coupled with tandem mass spectrometry method for quantification of aripiprazole in implantable microparticles in rat plasma from pharmacokinetic study

“…The detailed study on analytical methods available on ARIP is particularly focused on quantification of the drug which includes HPLC [7], CE [8], and LC-MS/MS [9][10][11][12]. However, all these methods have one or more drawbacks to qualify for an ideal and robust method of analysis of ARIP in the plasma or serum.…”

“…The method described by Liang et al, 2012 had a higher flow rate of the mobile phase with gradient elution, supported by a higher organic fraction, though leading to the lower recovery of ARIP. A larger particle-sized stationary phase was used by Ravinder et al, in 2012, andSun et al, in 2018, in two separate analytical methods for ARIP. The earlier one also has issues like higher ACN content, low recovery, and longer retention of ARIP.…”