Simultaneous cell disruption and lipid extraction of wet aurantiochytrium sp. KRS101 using a high shear mixer

Abstract

Microalgae are regarded as a promising source of biofuels, and the concept of a microalgae-based biorefinery has attracted increasing attention in recent years. From an economic perspective, however, the process remains far from competitive with fossil fuels. This is particularly true of lipid extraction, due in part to the energy-intensive drying step. As a result, wet extraction methods have been studied as an economic alternative. In the present study, a novel extraction approach which utilizes high shear stress mixing was adopted and demonstrated for simultaneous lipid extraction and cell disruption to enable the retrieval of lipids directly from concentrated wet biomass. When a high shear mixer (HSM) was used to extract lipid from a dense biomass (> 350 g/L) of the oleaginous algae Aurantiochytrium sp., it exhibited a yield of esterifiable lipids which exceeded 80% in 10 min at 15,000 rpm with various solvent types. The HSM was found to improve the lipid yields substantially with solvents less miscible with either lipids or water, such that the range of Hansen solubility parameters for the usable solvents became 3.3 times wider (14.9-26.5 MPa). The HSM, which appeared effectively to loosen the water barrier that prevents solvent molecules from penetrating through the cell envelope, was found to be more efficient with hexane, hexane/isopropanol, and ethanol, all of which showed nearly identical lipid yields compared to the dry extraction process. The HSM can, indeed, offer a powerful mechanical means of lipid extraction with non-polar and less toxic solvents from wet biomass.

High-Throughput Biochemical Fingerprinting of Oleaginous Aurantiochytrium sp. Strains by Fourier Transform Infrared Spectroscopy (FT-IR) for Lipid and Carbohydrate Productions

“…A wet extraction method was directly exerted on the wet cells of Aurantiochytrium sp. for lipid extraction and analysis through a high shear mixer (HSM) [11]. Otherwise, pyrolysis of cell based on the thermogravimetric analysis and ultrasound-assisted transesterification in wet Aurantiochytrium sp.…”

Optimasi Penggunaan High Shear Mixer pada Pembuatan Fraksi Alkaloid dari Daun Awar-awar (Ficus septica) dengan Desain Faktorial

“…Metode fraksinasi ataupun ekstraksi dengan menggunakan HSM juga terbukti efektif untuk sampel tanaman atau bahan alam lain. Sebelumnya, Kwak et al 26 menunjukkan bahwa HSM efektif digunakan dalam pemisahan lemak dari alga hijau. Temuan terkini juga menunjukkan efektivitas HSM dalam memisahkan karbohidrat berbobot molekul rendah dalam serbuk umbi ketela pohon.…”

Extraction of lipids from algae using supercritical carbon dioxide

“…The expeller press uses mechanical crushing in an oil press, while bead beating relies on a grinding mechanism. Shaking vessels and agitated beads are types of beads that break cells by shaking the culture vessel [75][76][77][78][79][80]. High hardness and density of zirconia-silica, zirconium oxide, or titanium carbide beads improve the disruption rate and extraction efficiency [81,82].…”