Extraction of lipids from algae using supercritical carbon dioxide

Patel, Alok; Μάτσακας, Λεωνίδας; Sartaj, Km; Chandra, Rampalli Viswa

doi:10.1016/b978-0-12-817388-6.00002-7

Cited by 14 publications

(5 citation statements)

References 147 publications

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“…Apart from enhancing the lipid extraction, the application of pretreatment can also allow lipid extraction directly from wet biomass [301]. Generally, the pretreatment techniques are divided into mechanical and non-mechanical methods, with the non-mechanical methods to be further divided into physical, chemical, and enzymatic methods [285,290,[302][303][304]. Currently, various pretreatment methods have been employed in laboratory-scale, such as high-pressure homogenization, bead beating, microwave, ultrasonication, osmotic shocks, and autoclaving.…”

Section: Pretreatment For Enhancement Of Lipid Extractionmentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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Microorganisms are known to be natural oil producers in their cellular compartments. Microorganisms that accumulate more than 20% w/w of lipids on a cell dry weight basis are considered as oleaginous microorganisms. These are capable of synthesizing vast majority of fatty acids from short hydrocarbonated chain (C6) to long hydrocarbonated chain (C36), which may be saturated (SFA), monounsaturated (MUFA), or polyunsaturated fatty acids (PUFA), depending on the presence and number of double bonds in hydrocarbonated chains. Depending on the fatty acid profile, the oils obtained from oleaginous microorganisms are utilized as feedstock for either biodiesel production or as nutraceuticals. Mainly microalgae, bacteria, and yeasts are involved in the production of biodiesel, whereas thraustochytrids, fungi, and some of the microalgae are well known to be producers of very long-chain PUFA (omega-3 fatty acids). In this review article, the type of oleaginous microorganisms and their expertise in the field of biodiesel or omega-3 fatty acids, advances in metabolic engineering tools for enhanced lipid accumulation, upstream and downstream processing of lipids, including purification of biodiesel and concentration of omega-3 fatty acids are reviewed.

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Section: Pretreatment For Enhancement Of Lipid Extractionmentioning

confidence: 99%

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

et al. 2020

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“…Recently, new solvent-free lipid extraction methods have been developed, such as supercritical fluid extraction (SFE). This technique uses supercritical fluids-of which the most used is CO 2 -which are a composite form of gas and liquid properties, existing above a critical temperature and pressure [276]. Supercritical CO 2 is chemically inert, safe, low cost, nontoxic, and it is environmentally feasible with no access to organic solvents, allowing a greater purity of the final product [277].…”

Section: Extraction Purification and Stability Of Pufasmentioning

confidence: 99%

Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture

et al. 2021

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Microalgae have a great potential for the production of healthy food and feed supplements. Their ability to convert carbon into high-value compounds and to be cultured in large scale without interfering with crop cultivation makes these photosynthetic microorganisms promising for the sustainable production of lipids. In particular, microalgae represent an alternative source of polyunsaturated fatty acids (PUFAs), whose consumption is related to various health benefits for humans and animals. In recent years, several strategies to improve PUFAs’ production in microalgae have been investigated. Such strategies include selecting the best performing species and strains and the optimization of culturing conditions, with special emphasis on the different cultivation systems and the effect of different abiotic factors on PUFAs’ accumulation in microalgae. Moreover, developments and results obtained through the most modern genetic and metabolic engineering techniques are described, focusing on the strategies that lead to an increased lipid production or an altered PUFAs’ profile. Additionally, we provide an overview of biotechnological applications of PUFAs derived from microalgae as safe and sustainable organisms, such as aquafeed and food ingredients, and of the main techniques (and their related issues) for PUFAs’ extraction and purification from microalgal biomass.

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“…The lipids used are mainly fish and vegetable oils. The production of these oils has been questioned environmentally and is not viable over time, considering the projected demand for their use in the coming years [1,11]. For this reason, there has been increasing interest in discovering sustainable and cost-effective new alternative lipid sources for use in the diet of cultured aquatic animals [11,12].…”

Section: Introductionmentioning

confidence: 99%

“…Several authors have reported lipid accumulation in yeasts [11,13,14] with the most promising species for fat production being the following: Rhodosporidium toruloides, Lipomyces starkeyi, Lipomyces tetrasporus, Cutaneotrichosporon curvatum, Candida diddensiae, Metschnikowia reukaufii, Candida tropicalis, Solicoccosyma terricola, Naganishia albida, Papiliotrema laurentii, Rhodotorula glutinis, Rhodotorula graminis, Rhodotorula mucilaginosa, Apiotrichum domesticum, Trichosporon asahii, Tausonia pullulans, Yarrowia lipolytica and Schwanniomyces etchellsii [15][16][17][18][19]. Most of these yeasts accumulate lipids mainly in the cytoplasmic membrane (about 25% of the cell dry weight) in the form of triglycerides (TGs) [20], noting that the major production of these TGs is under conditions of nutrient limitation such as nitrogen, phosphorus and an excess carbon source [21][22][23].…”

Section: Introductionmentioning

confidence: 99%

Lipid Production from Native Oleaginous Yeasts Isolated from Southern Chilean Soil Cultivated in Industrial Vinasse Residues

Díaz-Navarrete,

Marileo,

Madrid

et al. 2023

Microorganisms

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In this research, six strains of oleaginous yeasts native to southern Chile were analyzed for their biotechnological potential in lipid accumulation. For this purpose, the six strains, named PP1, PP4, PR4, PR10, PR27 and PR29, were cultivated in a nitrogen-deficient synthetic mineral medium (SMM). Then, two strains were selected and cultivated in an industrial residual “vinasse”, under different conditions of temperature (°C), pH and carbon/nitrogen (C/N) ratio. Finally, under optimized conditions, the growth kinetics and determination of the lipid profile were evaluated. The results of growth in the SMM indicate that yeasts PP1 and PR27 presented biomass concentrations and lipid accumulation percentages of 2.73 and 4.3 g/L of biomass and 36.6% and 45.3% lipids, respectively. Subsequently, for both strains, when cultured in the residual vinasse under optimized environmental conditions, biomass concentrations of 14.8 ± 1.51 g/L (C/N 80) and 15.83 ± 0.57 g/L (C/N 50) and lipid accumulations of 28% and 30% were obtained for PP1 and PR27, respectively. The composition of the triglycerides (TGs), obtained in the culture of the yeasts in a 2 L reactor, presented 64.25% of saturated fatty acids for strain PR27 and 47.18% for strain PP1. The saturated fatty acid compositions in both strains are mainly constituted of fatty acids, myristic C 14:0, heptadecanoic C 17:0, palmitic C 16:0 and stearic C 18:0, and the monounsaturated fatty acids constituted of oleic acid C 18:1 (cis 9) (28–46%), and in smaller amounts, palmitoleic acid and heptadecenoic acid. This work demonstrates that the native yeast strains PP1 and PR27 are promising strains for the production of microbial oils similar to conventional vegetable oils. The potential applications in the energy or food industries, such as aquaculture, are conceivable.

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Extraction of lipids from algae using supercritical carbon dioxide

Cited by 14 publications

References 147 publications

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

An Overview of Potential Oleaginous Microorganisms and Their Role in Biodiesel and Omega-3 Fatty Acid-Based Industries

Highly Valuable Polyunsaturated Fatty Acids from Microalgae: Strategies to Improve Their Yields and Their Potential Exploitation in Aquaculture

Lipid Production from Native Oleaginous Yeasts Isolated from Southern Chilean Soil Cultivated in Industrial Vinasse Residues

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