First Diagnosed Case of Camelpox Virus in Israel

Erster, Oran; Melamed, Sharon; Paran, Nir; Weiss, Shay; Khinich, Yevgeny; Gelman, Boris; Solomony, Aharon; Laskar-Levy, Orly

doi:10.3390/v10020078

Cited by 12 publications

(6 citation statements)

References 27 publications

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“…F4018, Sigma, St. Louis, MO, USA) was used as a secondary antibody for WNV detection in IFA experiments. In-house production of rabbit anti-Vaccinia antibodies and mouse monoclonal anti-West Nile Virus antibodies were used in this study [ 17 , 18 , 19 , 20 ]. The antibodies were conjugated to Alexa 488 (Alexa Fluor 488 protein labeling kit, Cat.…”

Section: Methodsmentioning

confidence: 99%

A Cell-Based Capture Assay for Rapid Virus Detection

Milrot

Makdasi

Politi

et al. 2020

Viruses

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Routine methods for virus detection in clinical specimens rely on a variety of sensitive methods, such as genetic, cell culture and immuno-based assays. It is imperative that the detection assays would be reliable, reproducible, sensitive and rapid. Isolation of viruses from clinical samples is crucial for deeper virus identification and analysis. Here we introduce a rapid cell-based assay for isolation and detection of viruses. As a proof of concept several model viruses including West Nile Virus (WNV), Modified Vaccinia Ankara (MVA) and Adenovirus were chosen. Suspended Vero cells were employed to capture the viruses following specific antibody labeling which enables their detection by flow cytometry and immuno-fluorescence microscopy assays. Using flow cytometry, a dose response analysis was performed in which 3.6e4 pfu/mL and 1e6 pfu/mL of MVA and WNV could be detected within two hours, respectively. When spiked to commercial pooled human serum, detection sensitivity was slightly reduced to 3e6 pfu/mL for WNV, but remained essentially the same for MVA. In conclusion, the study demonstrates a robust and rapid methodology for virus detection using flow cytometry and fluorescence microscopy. We propose that this proof of concept may prove useful in identifying future pathogens.

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Section: Methodsmentioning

confidence: 99%

A Cell-Based Capture Assay for Rapid Virus Detection

Milrot

Makdasi

Politi

et al. 2020

Viruses

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“…Among the viral diseases affecting camels, camelpox is an economically important disease, notifiable to OIE (245)(246)(247)(248)(249). (240).…”

Section: Burnetii Still Neglected In the Oie List Of Zoonotic Pathmentioning

confidence: 99%

Coxiella burnetii in Dromedary Camels (Camelus dromedarius): A Possible Threat for Humans and Livestock in North Africa and the Near and Middle East?

et al. 2020

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The "One Health" concept recognizes that human health is connected to animal health and to the ecosystems. Coxiella burnetii-induced human Q fever is one of the most widespread neglected zoonosis. The main animal reservoirs responsible for C. burnetii transmission to humans are domesticated ruminants, primarily goats, sheep, and cattle. Although studies are still too sparse to draw definitive conclusions, the most recent C. burnetii serosurvey studies conducted in herds and farms in Africa, North Africa, Arabian Peninsula, and Asia highlighted that seroprevalence was strikingly higher in dromedary camels (Camelus dromedarius) than in other ruminants. The C. burnetii seroprevalence in camel herds can reach more than 60% in Egypt, Saudi Arabia, and Sudan, and 70 to 80% in Algeria and Chad, respectively. The highest seroprevalence was in female camels with a previous history of abortion. Moreover, C. burnetii infection was reported in ticks of the Hyalomma dromedarii and Hyalomma impeltatum species collected on camels. Even if dromedary camels represent <3% of the domesticated ruminants in the countries of the Mediterranean basin Southern coast, these animals play a major socioeconomic role for millions of people who live in the arid zones of Africa, Middle East, and Asia. In Chad and Somalia, camels account for about 7 and 21% of domesticated ruminants, respectively. To meet the growing consumers demand of camel meat and milk (>5 million tons/year of both raw and pasteurized milk according to the Food and Agriculture Organization) sustained by a rapid increase of population (growth rate: 2.26-3.76 per year in North Africa), dromedary camel breeding tends to increase from the Maghreb to the Arabic countries. Because of possible long-term persistence of C. burnetii in camel hump adipocytes, this pathogen could represent a threat for herds and breeding farms and ultimately for public health. Because this review highlights a hyperendemia of C. burnetii in dromedary camels, a proper screening of herds and breeding farms for C. burnetii is urgently needed in countries where camel breeding is on the rise. Moreover, the risk of C. burnetii transmission from camel to human should be further evaluated.

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“…No infections in husbandry personnel were reported. In order to identify the etiologic agent, skin samples from representative camels were subjected to various diagnostic tests (6). Transmission electron microscopy analysis revealed the presence of an OPV-like virus, based on its characteristic brick shape.…”

Section: Announcementmentioning

confidence: 99%

“…The virus from the skin lesions induced a cytopathic effect in Vero cells, which were subsequently positively stained by an orthopox-specific antibody. The final identification of the virus was accomplished by two quantitative PCR (qPCR) tests (6, 7). Here, we report, for the first time, the whole-genome sequencing of a CMLV case in Israel.…”

Section: Announcementmentioning

confidence: 99%