Gold–carbon dots for the intracellular imaging of cancer-derived exosomes

Jiang, Xiaoyue; Zong, Shenfei; Chen, Chen; Zhang, Yizhi; Wang, Zhuyuan; Cui, Yiping

doi:10.1088/1361-6528/aaaf14

Cited by 48 publications

(48 citation statements)

References 44 publications

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“…Other authors have encapsulated quantum dots (QDs) into EVs. These strongly fluorescent materials could be useful for variety of fluorescence analysis techniques, because of their enhanced stability and the possibility of using them simultaneously for photodynamic therapy [138].…”

Section: Tracking Studies Of Evsmentioning

confidence: 99%

Exploiting the Natural Properties of Extracellular Vesicles in Targeted Delivery towards Specific Cells and Tissues

et al. 2020

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Extracellular vesicles (EVs) are important mediators of intercellular communication that participate in many physiological/pathological processes. As such, EVs have unique properties related to their origin, which can be exploited for drug delivery applications in cell regeneration, immunosuppression, inflammation, cancer treatment or cardioprotection. Moreover, their cell-like membrane organization facilitates uptake and accumulation in specific tissues and organs, which can be exploited to improve selectivity of cargo delivery. The combination of these properties with the inclusion of drugs or imaging agents can significantly improve therapeutic efficacy and selectivity, reduce the undesirable side effects of drugs or permit earlier diagnosis of diseases. In this review, we will describe the natural properties of EVs isolated from different cell sources and discuss strategies that can be applied to increase the efficacy of targeting drugs or other contents to specific locations. The potential risks associated with the use of EVs will also be addressed.

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Section: Tracking Studies Of Evsmentioning

confidence: 99%

Exploiting the Natural Properties of Extracellular Vesicles in Targeted Delivery towards Specific Cells and Tissues

et al. 2020

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“…Exosomes were the imaging target of the study of Jiang et al [78]. They produced gold-CNDs by combining carbon dots and gold nanoclusters, which then they modified with rabbit anti-HER2.…”

Section: Target-specific Imaging Applications For Eukaryotic Cellsmentioning

confidence: 99%

Bioimaging Applications of Carbon Nanodots: A Review

Kasouni

Chatzimitakos

Stalikas

2019

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Carbon nanodots (CNDs) is the newest member of carbon-based nanomaterials and one of the most promising for the development of new, advanced applications. Owing to their unique and unparalleled physicochemical and photoluminescent properties, they are considered to be a rising star among nanomaterials. During the last decade, many applications have been developed based on CNDs. Among others, they have been used as bioimaging agents to label cells and tissues. In this review, we will discuss the advancements in the applications of CNDs in in the field of imaging, in all types of organisms (i.e., prokaryotes, eukaryotes, and animals). Selective imaging of one type of cells over another, imaging of (bio)molecules inside cells and tumor-targeting imaging are some of the studies that will be discussed hereafter. We hope that this review will assist researchers with obtaining a holistic view of the developed applications and hit on new ideas so that more advanced applications can be developed in the near future.

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“…how the cells remain physically attached to one another after cell division) can be made. Based on these results, we can assume that the NPCDs could be effectively used for cancer cell imaging 12 , 61 .…”

Section: Discussionmentioning

confidence: 82%

Multifunctional N-P-doped carbon dots for regulation of apoptosis and autophagy in B16F10 melanoma cancer cells and in vitro imaging applications

et al. 2020

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Rationale: The present study reports the multifunctional anticancer activity against B16F10 melanoma cancer cells and the bioimaging ability of fluorescent nitrogen-phosphorous-doped carbon dots (NPCDs). Methods: The NPCDs were synthesized using a single-step, thermal treatment and were characterized by TEM, XPS, fluorescence and UV-Vis spectroscopy, and FTIR analysis. The anticancer efficacy of NPCDs was confirmed by using cell viability assay, morphological evaluation, fluorescent live-dead cell assay, mitochondrial potential assay, ROS production, RT-PCR, western-blot analysis, siRNA transfection, and cellular bioimaging ability. Results: The NPCDs inhibited the proliferation of B16F10 melanoma cancer cells after 24 h of treatment and induced apoptosis, as confirmed by the presence of fragmented nuclei, reduced mitochondrial membrane potential, and elevated levels of reactive oxygen species. The NPCDs treatment further elevated the levels of pro-apoptotic factors and down-regulated the level of Bcl2 (B-cell lymphoma 2) that weakened the mitochondrial membrane, and activated proteases such as caspases. Treatment with NPCDs also resulted in dose-dependent cell cycle arrest, as indicated by reduced cyclin-dependent kinase (CDK)-2, -4, and -6 protein levels and an enhanced level of p21. More importantly, the NPCDs induced the activation of autophagy by upregulating the protein expression levels of LC3-II and ATG-5 (autophagy-related-5) and by downregulating p62 level, validated by knockdown of ATG-5. Additionally, owing to their excellent luminescence property, these NPCDs were also applicable in cellular bioimaging, as evidenced by the microscopic fluorescence imaging of B16F10 melanoma cells. Conclusion: Based on these findings, we conclude that our newly synthesized NPCDs induced cell cycle arrest, autophagy, and apoptosis in B16F10 melanoma cells and presented good cellular bioimaging capability.

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Gold–carbon dots for the intracellular imaging of cancer-derived exosomes

Cited by 48 publications

References 44 publications

Exploiting the Natural Properties of Extracellular Vesicles in Targeted Delivery towards Specific Cells and Tissues

Exploiting the Natural Properties of Extracellular Vesicles in Targeted Delivery towards Specific Cells and Tissues

Bioimaging Applications of Carbon Nanodots: A Review

Multifunctional N-P-doped carbon dots for regulation of apoptosis and autophagy in B16F10 melanoma cancer cells and in vitro imaging applications

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