Antibacterial activity and dentin bonding ability of combined use of Clearfil SE Protect and sodium hypochlorite

Abstract

The aim of this study was to evaluate the antibacterial activity and dentin bonding ability of a commercial self-etch adhesive Clearfil SE Protect (Kuraray Noritake Dental, Tokyo, Japan) in combination with sodium hypochlorite (NaOCl). Agar disc diffusion tests and measurement of minimum inhibitory/bactericidal concentrations (MIC/MBC) against Streptococcus mutans were performed to evaluate antibacterial effects. The mixture solution of 5.25% NaOCl and the primer of Clearfil SE Protect demonstrated less antibacterial activity than primer only. In microtensile bond strength tests using non-carious human molars, pretreatment with 5.25% NaOCl aqueous solution had no influence on the bond strength of Clearfil SE Protect. These results indicate that pretreatment with NaOCl does not influence the bonding ability of Clearfil SE Protect, while their combined use does not enhance cavity disinfecting effects.

Investigating a Commercial Functional Adhesive with 12-MDPB and Reactive Filler to Strengthen the Adhesive Interface in Eroded Dentin

“…These results echo the ones found in a study which also assessed the durability of CFP and found it to be stable over time [ 68 ]. Apparently, this group was less vulnerable to a hybrid layer breakdown, which is pointed out in the literature [ 15 , 23 , 50 , 54 ]. The accepted theory for this trend seems to be linked to 12-MDPB’s QAC group, which may be able to inhibit matrix metalloproteinases (MMPs) and thus, protect the hybrid layer, as previously investigated by Almahdy et al (2012), Tezvergil-Mutluay et al (2015), and Liu et al (2011) [ 23 , 32 , 69 ].…”

“…Given their respective composition, Clearfil SE Bond 2 is thus the ideal control when testing Clearfil SE Protect. 12-MDPB is described as a powerful antibacterial agent, able to co-polymerize within the organic matrix to other monomers [ 24 , 51 , 52 , 53 ], and provide an inhibitory action against oral Streptococci, without compromising the quality of the adhesive interface [ 15 , 23 , 50 , 54 ]. This antibacterial action has proven to be a promising strategy regarding treatment longevity, as proposed by Hashimoto et al (2018) [ 55 ].…”

Antibacterial effect of titanium dioxide-doped phosphate glass microspheres filled total-etch dental adhesive on S. mutans biofilm

“…Having dental adhesives with antibacterial properties is a viable option to suppress the bacterial colonization around restorations and prevent the replacement of restorations due to recurrence of caries. Some commercially available dental adhesives are modified by the inclusion of antibacterial agents such as glutraldehyde (eg, Gluma 2 Bond) (25), chlorhexidine (eg, Peak Universal Bond) (26) and methacryloyloxydodecylpyridinium bromide (MDPB) (eg, Clearfile SE Protect) (27). Regardless of the antibacterial action of these adhesives, none of them showed remineralization potential (ie, ability to remineralize dentin in deep areas where the adhesives might fail to penetrate).…”

Commercially Available Ion-Releasing Dental Materials and Cavitated Carious Lesions: Clinical Treatment Options

“… Adhesive system options including antiseptic molecules: Adhesive systems with 0.2% of CHX having anti-MMPs activities or including remineralizing modified calcium phosphate [ 40 , 54 , 55 ]. Adhesive systems with antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB): antibacterial activity based upon MDPB against S. mutans, L. casei and A. naeslundii, and the ability to disinfect cavities containing residual bacteria [ 54 , 56 , 57 ]. …”

“…Adhesive systems with antibacterial monomer 12-methacryloyloxydodecylpyridinium bromide (MDPB): antibacterial activity based upon MDPB against S. mutans, L. casei and A. naeslundii, and the ability to disinfect cavities containing residual bacteria [ 54 , 56 , 57 ].…”