MicroRNA‐22 suppresses cell proliferation, migration and invasion in oral squamous cell carcinoma by targeting NLRP3

Abstract

MicroRNAs (miRNAs) have been implicated as important regulators of carcinogenesis and tumor development. Recently, microRNA-22 (miR-22) has been reported to be a cancer-related miRNA in several types of tumors. In this study, we aimed to investigate the role of miR-22 in oral squamous cell carcinoma (OSCC). We found that miR-22 expression was significantly decreased in OSCC tissues compared with that in the adjacent noncancerous tissues. Furthermore, lentivirus-mediated miR-22 overexpression markedly reduced OSCC cell viability, migration and invasion, whereas miR-22 inhibitor promoted these parameters. Mechanistically, NLR family pyrin domain containing three (NLRP3) was identified as a direct target of miR-22. miR-22 expression was inversely correlated with NLRP3 expression both in OSCC tissues and cell lines. Moreover, overexpression of miR-22 in OSCC cells could reverse the tumor-promoting effect of the activated NLRP3 inflammasome and vice versus. Therefore, our results indicate that miR-22 may play a suppressive role in OSCC by targeting NLRP3, which offer new insights into the molecular mechanisms of the growth and metastasis of OSCC.

Evidence and perspective for the role of the NLRP3 inflammasome signaling pathway in ischemic stroke and its therapeutic potential (Review)

“…Many NLRP3 inhibitors found to have benefits following ischemic stroke were identified as natural compounds, and further research should elucidate the relationship between molecular structure and anti-NLRP3 inflammasome function. Furthermore, several microRNAs have been demonstrated to influence the NLRP3 inflammasome pathway, including miR-22 (117), miR-132 (118), and long non-coding RNA XLOc_000647 (119). Further studies should consider the modulation of the NLRP3 inflammasome post-stroke via epigenetic approaches.…”

MicroRNA‐16 functions as a tumor‐suppressor gene in oral squamous cell carcinoma by targeting AKT3 and BCL2L2

“…Dysregulation of miRNAs has been reported to be strongly associated with the progression and prognosis of OSCC (Tu, Lin, & Chang, 2013). In particular, a number of miRNAs, for example, miR-22, miR-124, miR-433, and miR-375 (Feng, Luo, Wang, Zhang, & Chen, 2018;Hunt, Jones, Hinsley, Whawell, & Lambert, 2011;Wang et al, 2017;Zhang et al, 2017), have been reported to inhibit OSCC cell growth, migration, and apoptosis, and have been recognized as novel targets in OSCC diagnosis and therapy.…”

<p>MicroRNA-139 Suppresses the Tumorigenicity of Triple Negative Breast Cancer Cells by Targeting SOX8</p>

“…Cells were cultured 1 day before transfection and inoculated into 6-well plates for lentivirus transfection. 15 Full length SOX8 cDNA cloned into pcDNA3.1 mammalian expression plasmid (Shanghai GeneChem Co., Ltd, China). TNBC cells (HCC1806 and BT549) were divided into the following five groups according to treatment: 1) blank control group (BC group, no transfection), 2) SOX8 overexpression group (SOX8 group, cells were transfected with SOX8 pcDNA3.1 using Lipofectamine 3000), 3) SOX8 overexpression negative control group (NC1 group, cells transfected with SOX8 negative control), 4) SOX8 silencing group (si-S group, stable shSOX8 cells were constructed using short hairpin RNA (shRNA) with GeneChem), and 5) SOX8 silencing negative control group (NC2 group, cells transfected with shSOX8 negative control).…”