The efficacy of basic fibroblast growth factor‐loaded poly(lactic‐co‐glycolic acid) nanosheet for mouse wound healing

Abstract

Although human recombinant basic fibroblast growth factor (bFGF) is widely used for wound healing, daily treatment with bFGF is required because of its short half-life. An effective controlled-release system of bFGF is, therefore, desired in clinical settings. To investigate the efficacy of a bFGF-loaded nanosheet for wound healing, focusing on the controlled-release of bFGF, bFGF-loaded poly(lactic-co-glycolic acid) (PGLA) nanosheets were developed, and their in vitro release profile of bFGF and their in vivo efficacy for wound healing were examined. A polyion complex of positively charged human recombinant bFGF and negatively charged alginate was sandwiched between PLGA nanosheets (70 nm thick for each layer). The resulting bFGF-loaded nanosheet robustly adhered to silicon skin by observation using a microscratch test. bFGF was gradually and continuously released over three days in an in vitro incubation study. Treatment with the bFGF-loaded nanosheets (every 3 day for 15 days) as well as with a conventional bFGF spray effectively promoted wound healing of mouse dorsal skin defects with accelerated tissue granulation and angiogenesis, although the dose of bFGF used in the treatment with the bFGF nanosheets was approximately 1/20 of the sprayed bFGF. In conclusion, we developed a bFGF-loaded nanosheet that sustained a continuous release of bFGF over three days and effectively promoted wound healing in mice.

Angiogenic efficacy of ASC spheroids filtrated on porous nanosheets for the treatment of a diabetic skin ulcer

“…The spheroid‐covered nanosheet had the most contracted skin defects at 14 days (Figure 4B). The increase in the defect area at Day 3 observed in all samples would occur by the contraction of the surrounding skin because of skin removal; this phenomenon was previously reported 37 . The transplanted spheroids would secrete VEGF and stimulate the defect surface, that promotes the secretion of exudate by the paracrine effect 2 .…”

Evaluation of sustained ciprofloxacin release of biodegradable electrospun gelatin/poly(glycerol sebacate) mat membranes for wound dressing applications

“…Zhang et al porous‐fabricated PGS scaffolds as skin substitutes . Aoki et al prepared fibroblast growth factor‐embedded poly(lactic‐ co ‐glycolic acid) nanosheet for wound healing …”

The immunologic changes during different phases of intestinal anastomotic healing

“…The bFGF is a multiple potential glycoprotein that promotes various cells such as fibroblasts, keratinocytes, and endothelial cells. Because of its mitogenic and angiogenic characteristics, the bFGF plays an important role in inducing tissue remodeling and wound healing 126,127 …”

“…Because of its mitogenic and angiogenic characteristics, the bFGF plays an important role in inducing tissue remodeling and wound healing. 126,127…”