Characterization of Novel Gene Yr79 and Four Additional Quantitative Trait Loci for All-Stage and High-Temperature Adult-Plant Resistance to Stripe Rust in Spring Wheat PI 182103

Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici, is an important disease of wheat worldwide. Exploring new resistance genes is essential for breeding resistant wheat cultivars. PI 182103, a spring wheat landrace originally from Pakistan, has shown a high level of resistance to stripe rust in fields for many years, but genes for resistance to stripe rust in the variety have not been studied. To map the resistance gene(s) in PI 182103, 185 recombinant inbred lines (RILs) were developed from a cross with Avocet Susceptible (AvS). The RIL population was genotyped with simple sequence repeat (SSR) and single nucleotide polymorphism markers and tested with races PST-100 and PST-114 at the seedling stage under controlled greenhouse conditions and at the adult-plant stage in fields at Pullman and Mt. Vernon, Washington under natural infection by the stripe rust pathogen in 2011, 2012, and 2013. A total of five quantitative trait loci (QTL) were detected. QyrPI182103.wgp-2AS and QyrPI182103.wgp-3AL were detected at the seedling stage, QyrPI182103.wgp-4DL was detected only in Mt. Vernon field tests, and QyrPI182103.wgp-5BS was detected in both seedling and field tests. QyrPI182103.wgp-7BL was identified as a high-temperature adult-plant resistance gene and detected in all field tests. Interactions among the QTL were mostly additive, but some negative interactions were detected. The 7BL QTL was mapped in chromosomal bin 7BL 0.40 to 0.45 and identified as a new gene, permanently designated as Yr79. SSR markers Xbarc72 and Xwmc335 flanking the Yr79 locus were highly polymorphic in various wheat genotypes, indicating that the molecular markers are useful for incorporating the new gene for potentially durable stripe rust resistance into new wheat cultivars.

Mapping QTL for Resistance to New Virulent Races of Wheat Stripe Rust from Two Argentinean Wheat Cultivars

“…6). QYr.wgp-4D, identified in PI 182103, was mapped on the centromeric region of chromosome 4D, and its long arm border (IWA4044; Feng et al, 2018) was mapped >150 Mb proximal to QYr.ucw-4DL, indicating that they do not correspond to the same resistance gene. QYr.caas-4DL, identified in the cultivar 'Bainong 64' (Ren et al, 2012), was associated with leaf rust and powdery mildew resistance, suggesting that it may be Lr67/Yr46 (Ren et al, 2015).…”

Characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in Northern Chinese wheat landraces

“…In contrast, APR is only effective at adult plant stages when warm weather is prevalent, and is usually non-race specific and quantitatively inherited, and more likely to be durable [11]. Previously, 80 Yr genes for stripe rust resistance have been identified and formally named [12,13], however, the majority of these resistance genes are ineffective against new Pst races [14][15][16]. Therefore, identification of novel sources of resistance for deployment in breeding programs is a matter of urgency.…”

Transcriptomic Analysis Reveal the Molecular Mechanisms of Wheat Higher-Temperature Seedling-Plant Resistance to Puccinia striiformis f. sp. tritici

“…There have been many studies on temperature sensitive R genes against stripe rust, especially non-race-specific HTAP resistance, such as Yr36 (Fu et al, 2009 ; Bryant et al, 2014 ); Yr52 (Ren et al, 2012 ); Yr59 (Zhou et al, 2014 ), Yr62 (Lu et al, 2014 ), LrZH22 (Wang et al, 2016 ), and Yr79 (Feng et al, 2018 ). Previously cloned non-race-specific resistance genes to stripe rust and/or leaf rust, such as Yr18/Lr34 (Krattinger et al, 2009a ), Yr36 (Fu et al, 2009 ), and Yr46/Lr67 (Moore et al, 2015 ), do not contain NBS-LRR domains (Chen, 2013 ), in contrast to many race-specific resistance to stripe rust and leaf rust, such as Lr10 (Feuillet et al, 2003 ), Lr21 (Huang et al, 2003 ); Lr1 (Cloutier et al, 2007 ); Yr10 (Liu W. et al, 2014 ).…”