Characterization of molecular diversity and genome-wide association study of stripe rust resistance at the adult plant stage in Northern Chinese wheat landraces

Abstract

Background: Stripe rust is a serious fungal disease of wheat (Triticum aestivum L.) caused by Puccinia striiformis f. sp. tritici (Pst), which results in yield reduction and decreased grain quality. Breeding for genetic resistance to stripe rust is the most cost-effective method to control the disease. In the present study, a genome-wide association study (GWAS) was conducted to identify markers linked to stripe rust resistance genes (or loci) in 93 Northern Chinese wheat landraces, using Diversity Arrays Technology (DArT) and simple sequence repeat (SSR) molecular marker technology based on phenotypic data from two field locations over two growing seasons in China. Results: Seventeen accessions were verified to display stable and high levels of adult plant resistance (APR) to stripe rust via multi-environment field assessments. Significant correlations among environments and high heritability were observed for stripe rust infection type (IT) and disease severity (DS). Using mixed linear models (MLM) for the GWAS, a total of 32 significantly associated loci (P < 0.001) were detected. In combination with the linkage disequilibrium (LD) decay distance (6.4 cM), 25 quantitative trait loci (QTL) were identified. Based on the integrated map of previously reported genes and QTL, six QTL located on chromosomes 4A, 6A and 7D were mapped far from resistance regions identified previously, and represent potentially novel stripe rust resistance loci at the adult plant stage. Conclusions: The present findings demonstrated that identification of genes or loci linked to significant markers in wheat by GWAS is feasible. Seventeen elite accessions conferred with stable and high resistance to stripe rust, and six putative newly detected APR loci were identified among the 93 Northern Chinese wheat landraces. The results illustrate the potential for acceleration of molecular breeding of wheat, and also provide novel sources of stripe rust resistance with potential utility in the breeding of improved wheat cultivars.

Genomic Regions Associated With Stripe Rust Resistance Against the Egyptian Race Revealed by Genome-Wide Association Study

“…However, no available information about the reaction of these two genes against the Egyptian stripe rust race. In addition, many studies mapped novel QTLs controlling adult stage stripe rust resistance to this chromosome which con rming our ndings [45][46][47].…”

“…Some of these resistance genes were reported to resist the Egyptian race of stripe rust at the adult stage such as Yr1, Yr17, and Yr32 [44]. Some stripe rust resistance genes were mapped on chromosome 4A such as Yr51 and Yr60 [45]. However, no available information about the reaction of these two genes against the Egyptian stripe rust race.…”

Efficient anchoring of Erianthus&nbsp;arundinaceus chromatin introgressed into sugarcane by specific molecular markers

“…Nevertheless, these conventional methods used for development of speci c molecular markers are timeconsuming, laborious, and expensive (Devos and Gale 1992). In recent years, sequencing-based methods also can greatly facilitate the availability of species-speci c molecular markers, such as genotyping by sequencing (GBS) and the diversity arrays technology sequencing (DarT-seq), which were bene ted for some grass species such as maize, wheat, and rice (He et al 2014;Phung et al 2014;Yao et al 2019). Nevertheless, developing species-speci c molecular markers by using genomic tools were relatively expensive.…”

Breeding Progress in Seedling Resistance against Various Races of Stripe and Leaf Rust in European Bread Wheat

“…Accordingly, susceptibility to the latter races considerably However, minimal or no information about cultivars carrying these resistances is available. Furthermore, the differential set used in this study did not comprise all of the 80 Yr genes which have been identified for stripe rust [37]. A possible recombination between "Oakley,v7/Kranich" and the Warrior races could result in an increased level of stripe rust infections due to a high number of virulences.…”