MicroRNA-187 Inhibits Growth and Metastasis of Osteosarcoma by Downregulating S100A4

Xiao, Yi; Zhao, Quan; Du, Bo; Chen, Huayan; Dao-zheng, Zhou

doi:10.1080/07357907.2017.1415348

Cited by 17 publications

(15 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A miR-187 mimic was transfected into cells to overexpress miR-187, and then, the proliferation of the cells was measured by an MTS assay. The results indicated that miR-187 overexpression inhibited NSCLC cell proliferation, which is consistent with the results of Xiao et al [34] showing that miR-187 inhibits osteosarcoma cell proliferation in vitro and vivo, and Mao et al [19] who reported that the overexpression of miR-187-5p inhibits the growth and metastasis of NSCLC cells. Colony formation assay indicated that miR-187 had an anticlonogenic effect on cancer cells.…”

Section: Discussionsupporting

confidence: 88%

MiR-187 suppresses non-small-cell lung cancer cell proliferation by targeting FGF9

Liang

et al. 2019

Bioengineered

View full text Add to dashboard Cite

Non-small-cell lung cancer (NSCLC) is the main pathological type of lung cancer and has a low overall five-year survival rate. miR-187 has been reported to play major roles in various tumor types. In this study, we explored the impact of miR-187 on NSCLC. qRT-PCR results demonstrated that miR-187 expression is lower in NSCLC and cancer cells than normal tissues and normal lung cells. miR-187 expression levels are associated with tumor size, TNM stage and overall survival rate. MTS and colony formation assays showed that high miR-187 expression inhibits NSCLC cell proliferation and colony formation ability, and flow cytometry showed that miR-187 overexpression induces cell cycle arrest at the G0/G1 phase. A luciferase reporter assay showed that FGF9 is a target of miR-187. miR-187 overexpression reduces the expression of FGF9, cyclin D1 CDK4 and CDK6. Therefore, miR-187 may present a new NSCLC treatment target by regulates cyclins-related protein expression.

show abstract

Section: Discussionsupporting

confidence: 88%

MiR-187 suppresses non-small-cell lung cancer cell proliferation by targeting FGF9

Liang

et al. 2019

Bioengineered

View full text Add to dashboard Cite

show abstract

“…miR-187-5p has been widely studied in recent years as a tumor suppressor in cervical cancer [12,13], bone tumor [17], lung cancer [11,18], and urologic neoplasms [19]. It has been investigated that miR-187 could suppress S100A4 expression through binding with S100A4 mRNA 3′-UTR in osteosarcoma cells [17]. In lung cancer, miR-187 contributes to the initiation of metastasis through regulating mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3-kinase/protein kinase B (PI3K/PKB) pathways.…”

Section: Introductionmentioning

confidence: 99%

miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

Sun

Wang

Chen

et al. 2020

BioMed Research International

View full text Add to dashboard Cite

Osteoporosis (OP) is a common bone metabolic disease, the process of which is fundamentally irreversible. Therefore, the investigation into osteoblastic differentiation of bone marrow mesenchymal stem cells (BMSCs) will provide more clues for OP treatment. In the present study, we found that microRNA-187-5p (miR-187-5p) played a key role on osteoblastic differentiation, which was significantly upregulated during osteogenic differentiation of BMSCs in mice. Moreover, overexpression of miR-187-5p suppressed osteoblastic differentiation of BMSCs through increasing alkaline phosphatase (ALP), matrix mineralization, and levels of Osterix (OSX), and osteopontin (OPN) as well as runt-related transcription factor 2 (Runx2) in vitro. The results in vivo indicated that the upregulation of miR-187-5p enhanced the efficacy of new bone formation in the heterotopic bone formation assay. Luciferase reporter assay and western blot analysis revealed that miR-187-5p was involved in osteogenesis by targeting intracellular adhesion molecule 1 (ICAM-1). Furthermore, ICAM-1 silence inhibited osteoblastic differentiation of BMSCs. Taken together, our results suggested for the first time that miR-187-5p may promote osteogenesis by targeting ICAM-1, and provided a possible therapeutic target for bone metabolic diseases.

show abstract

“…Cumulative studies have demonstrated that expression levels of several microRNAs in various tumor tissue are up-or downregulated to different degrees, including in OS. 34,35 miR381-3p is an important member of the microRNAs, and has been shown to be involved in the regulation of a variety of human diseases, such as intestinal ischemia-reperfusion injury and chondrogenesis and cartilage degradation. 36,37 However, there are few records Compared with the NC-mimic group, 143B and HOS cells of the miR381-3p-mimic group had much lower CCND1 mRNA and protein expression.…”

Section: Discussionmentioning

confidence: 99%

<p>lncRNA FLVCR-AS1 promotes osteosarcoma growth by targeting miR381-3p/CCND1</p>

Yang¹,

He²,

Duan³

et al. 2020

OTT

View full text Add to dashboard Cite

Purpose: This article reports on FLVCR-AS1 effects on osteosarcoma (OS) growth. Methods: Tumor tissue and adjacent normal tissue of 48 OS patients were collected. HOS and 143B cells were transfected. Gene expression was examined with qRT-PCR and Western blot. CCK8 assays and cell cloning was performed to measure cell proliferation. Cell cycle and apoptosis were assessed. Luciferase-reporter gene assays and RNA pull-down tests were used to detect targeting relationships between genes. Results: Prominently higher FLVCR-AS1 expression was found in OS tissue and cells, and was associated with poor prognosis (P<0.05, P<0.01, or P<0.001). Compared with the siCtrl group, 143B and HOS cells of the siFLVCR-AS1 group had significantly lower OD 450 values and clone numbers and obviously higher percentages of cells in the G 1 phase and apoptosis (P<0.01 or P<0.001). miR381-3p expression was directly inhibited by FLVCR-AS1, and CCND1 expression was directly suppressed by miR381-3p. Compared with the FLVCR-AS1 group, 143B cells of the FLVCR-AS1 + miR381-3p mimic group and FLVCR-AS1 + siCCND1 group showed remarkably lower OD 450 values and clone numbers obviously higher apoptosis and percentage of cells in the G 1 phase (P<0.05, P<0.01, or P<0.001). Conclusion: FLVCR-AS1 promoted OS growth by upregulating CCND1 expression via downregulation of miR381-3p.

show abstract

MicroRNA-187 Inhibits Growth and Metastasis of Osteosarcoma by Downregulating S100A4

Cited by 17 publications

References 21 publications

MiR-187 suppresses non-small-cell lung cancer cell proliferation by targeting FGF9

MiR-187 suppresses non-small-cell lung cancer cell proliferation by targeting FGF9

miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

<p>lncRNA FLVCR-AS1 promotes osteosarcoma growth by targeting miR381-3p/CCND1</p>

Contact Info

Product

Resources

About