miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

Sun, Yi; Wang, Xin; Chen, Guanghua; Song, Chengyi; Ma, Xinnan; Fu, Yutuo; Feng, Chao; Yan, Jinglong

doi:10.1155/2020/6139469

Cited by 13 publications

(9 citation statements)

References 34 publications

(44 reference statements)

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“…26 Moreover, it has been described that ICAM-1 enhances migration of leukocytes and plays an independent role in VICs calcification 43 and ICAM-1 silencing inhibits osteoblastic differentiation in bone marrow mesenchymal stem cells. 44 These results were reinforced at the ex vivo level, showing the positive association between MR expression and ICAM-1 in AVs only in men. Thus, ICAM-1 upregulation triggered by Aldo/ MR in men VICs seem to play a role in AV calcification.…”

Section: Discussionmentioning

confidence: 91%

Sex-Related Signaling of Aldosterone/Mineralocorticoid Receptor Pathway in Calcific Aortic Stenosis

et al. 2022

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Background: There are sex differences in the pathophysiology of aortic valve (AV) calcification in patients with aortic stenosis, although the molecular and cellular mechanisms have not been elucidated. Aldosterone (Aldo) promotes proteoglycan synthesis in valve interstitial cells (VICs) from mitral valves via the mineralocorticoid receptor (MR). We investigated the influence of sex in the role of Aldo/MR pathway in AV alterations in patients with aortic stenosis. METHODS AND RESULTS: MR was expressed by primary aortic VICs and in AVs from patients with aortic stenosis. MR expression positively correlated with VIC activation markers in AVs from both sexes. However, MR expression was positively associated with molecules involved in AV calcification only in AV from men. Aldo enhanced VIC activation markers in cells from men and women. Interestingly, Aldo increased the expression of calcification markers only in VICs isolated from men. In female VICs, Aldo enhanced fibrotic molecules. MR antagonism (spironolactone) blocked all the above effects. Cytokine arrays showed ICAM (intercellular adhesion molecule)-1 and osteopontin to be specifically increased by Aldo in male VICs. In AVs from men, MR expression positively associated with both ICAM-1 (intercellular adhesion molecule-1) and osteopontin. Only in female VICs, estradiol treatment blocked Aldo-induced VICs activation, inflammation, and fibrosis. Conclusions: These findings demonstrate that the Aldo/MR pathway could play a role in early stages of aortic stenosis by promoting VICs activation, fibrosis, and ulterior calcification. Importantly, Aldo/MR pathway is involved in fibrosis in women and in early AV calcification only in men. Accordingly, MR antagonism emerges as a new sex-specific pharmacological treatment to prevent AV alterations.

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Section: Discussionmentioning

confidence: 91%

Sex-Related Signaling of Aldosterone/Mineralocorticoid Receptor Pathway in Calcific Aortic Stenosis

et al. 2022

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“…Intracellular adhesion molecule I (ICAM1) can direct bone remodeling by promoting osteoclast formation, which is vital in multiple inflammatory bone diseases. miR-187-5p promotes osteogenesis by targeting ICAM1 and thus is a potential therapeutic target for bone diseases ( Sun et al., 2020 ). The phosphatidylinositol three-kinase (PI3K)/AKT signaling pathway is another significant pathway that regulates bone metabolism.…”

Section: Mechanisms Of the Ncrna-mediated Regulation Of Osteogenic Si...mentioning

confidence: 99%

Non-coding RNA delivery for bone tissue engineering: Progress, challenges, and potential solutions

Guan¹,

Zhang²,

Wu³

2022

iScience

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“…It presumably does this by targeting the Fat Mass and Obesity-associated protein, showing enhanced osteogenic differentiation upon miR-149 overexpression (95). Primarily researched in the field of oncology, miR-187 has also shown to enhance osteoblastic differentiation by targeting Intercellular Adhesion Molecule 1, leading to enhanced expression levels of RUNX2, OSX, and OPN (96). An important regulator of MSC lineage differentiation is Chicken Ovalbumin Upstream Promoter-transcription factor 2 (COUP-TFII).…”

Section: Osteogenesis and Osteoblastogenesismentioning

confidence: 99%

miRNAs Related to Different Processes of Fracture Healing: An Integrative Overview

et al. 2021

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Fracture healing is a complex, dynamic process that is directed by cellular communication and requires multiple cell types, such as osteoblasts, osteoclasts, and immune cells. Physiological fracture healing can be divided into several phases that consist of different processes, such as angiogenesis, osteogenesis, and bone resorption/remodelling. This is needed to guarantee proper bone regeneration after fracture. Communication and molecular regulation between different cell types and within cells is therefore key in successfully orchestrating these processes to ensure adequate bone healing. Among others, microRNAs (miRNAs) play an important role in cellular communication. microRNAs are small, non-coding RNA molecules of ~22 nucleotides long that can greatly influence gene expression by post-transcriptional regulation. Over the course of the past decade, more insights have been gained in the field of miRNAs and their role in cellular signalling in both inter- and intracellular pathways. The interplay between miRNAs and their mRNA targets, and the effect thereof on different processes and aspects within fracture healing, have shown to be interesting research topics with possible future diagnostic and therapeutic potential. Considering bone regeneration, research moreover focusses on specific microRNAs and their involvement in individual pathways. However, it is required to combine these data to gain more understanding on the effects of miRNAs in the dynamic process of fracture healing, and to enhance their translational application in research, as well as in the clinic. Therefore, this review aims to provide an integrative overview on miRNAs in fracture healing, related to several key aspects in the fracture healing cascade. A special focus will be put on hypoxia, angiogenesis, bone resorption, osteoclastogenesis, mineralization, osteogenesis, osteoblastogenesis, osteocytogenesis, and chondrogenesis.

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miRNA-187-5p Regulates Osteoblastic Differentiation of Bone Marrow Mesenchymal Stem Cells in Mice by Targeting ICAM1

Cited by 13 publications

References 34 publications

Sex-Related Signaling of Aldosterone/Mineralocorticoid Receptor Pathway in Calcific Aortic Stenosis

Sex-Related Signaling of Aldosterone/Mineralocorticoid Receptor Pathway in Calcific Aortic Stenosis

Non-coding RNA delivery for bone tissue engineering: Progress, challenges, and potential solutions

miRNAs Related to Different Processes of Fracture Healing: An Integrative Overview

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