Effects of intragastrically-administered Tepary bean lectins on digestive and immune organs: Preclinical evaluation

Alatorre-Cruz, Julia María; Pita-López, Wendoline; López-Reyes, Rosa Guadalupe; Ferriz‐Martínez, Roberto Augusto; Cervantes-Jiménez, Ricardo; Carrillo, María de Jesús Guerrero; Vargas, Paola Jazmín Aranda; López‐Herrera, Gabriela; Rodríguez-Méndez, Adriana Jheny; Zamora-Arroyo, Aarón; Gutiérrez-Sánchez, Héctor; Souza, Tércia Reis de; Blanco‐Labra, Alejandro; García-Gasca, Teresa

doi:10.1016/j.toxrep.2017.12.008

Cited by 18 publications

(15 citation statements)

References 40 publications

(56 reference statements)

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“…Similar effects were observed with other Tepary bean lectin fractions on different colon cancer cell lines [19,20]. In vivo studies have reported that TBLF (50 mg/kg by intragastric administration for six weeks every third day) exhibits low toxicity, good tolerability and activates the immune system [21,22]. Between the observed adverse effects are loss of body weight, small intestinal villus and colonic crypt atrophy and exocrine pancreas hypertrophy, without systemic adverse effects.…”

Section: Introductionsupporting

confidence: 71%

Tepary Bean (Phaseolus acutifolius) Lectins Induce Apoptosis and Cell Arrest in G0/G1 by P53(Ser46) Phosphorylation in Colon Cancer Cells

et al. 2020

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A Tepary bean lectin fraction (TBLF) has been studied because it exhibits differential cytotoxic and anticancer effects on colon cancer. The present work focuses on the evaluation of the apoptotic mechanism of action on colon cancer cells. Initially, lethal concentrations (LC50) were obtained for the three studied cell lines (HT-29, RKO and SW-480). HT-29 showed the highest LC50, 10 and 100 times higher than that of RKO and SW-480 cells, respectively. Apoptosis was evaluated by flow cytometry, where HT-29 cells showed the highest levels of early and total apoptosis, caspases activity was confirmed and necrosis was discarded. The effect on cell cycle arrest was shown in the G0/G1 phase. Specific apoptosis-related gene expression was determined, where an increase in p53 and a decrease in Bcl-2 were observed. Expression of p53 gene showed the maximum level at 8 h with an important decrease at 12 and 24 h, also the phosphorylated p53(ser46) increased at 8 h. Our results show that TBLF induces apoptosis in colon cancer cells by p-p53(ser46) involvement. Further studies will focus on studying the specific signal transduction pathway.

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Section: Introductionsupporting

confidence: 71%

Tepary Bean (Phaseolus acutifolius) Lectins Induce Apoptosis and Cell Arrest in G0/G1 by P53(Ser46) Phosphorylation in Colon Cancer Cells

et al. 2020

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“…Furthermore, TBLF presents low toxicity and inhibits early tumorigenesis in rats with chemically induced colorectal cancer [19,20]. When administered intragastrically to rats, TBLF has been shown to have a stimulatory effect on the immune system and displays few side effects, such as atrophy in small intestine villus and colonic crypts and pancreatic hypertrophy, that can be partially reverted after a two-week rest period [21]. These results suggest the potential therapeutic use of Tepary bean lectins against colon cancer.…”

Section: Introductionmentioning

confidence: 79%

Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification

et al. 2020

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Herein, we report the production of a recombinant Tepary bean lectin (rTBL-1), its three-dimensional (3D) structure, and its differential recognition for cancer-type glycoconjugates. rTBL-1 was expressed in Pichia pastoris, yielding 316 mg per liter of culture, and was purified by nickel affinity chromatography. Characterization of the protein showed that rTBL-1 is a stable 120 kDa homo-tetramer folded as a canonical leguminous lectin with two divalent cations (Ca2+ and Mn2+) attached to each subunit, confirmed in its 3D structure solved by X-ray diffraction at 1.9 Å resolution. Monomers also presented a ~2.5 kDa N-linked glycan located on the opposite face of the binding pocket. It does not participate in carbohydrate recognition but contributes to the stabilization of the interfaces between protomers. Screening for potential rTBL-1 targets by glycan array identified 14 positive binders, all of which correspond to β1-6 branched N-glycans’ characteristics of cancer cells. The presence of α1-6 core fucose, also tumor-associated, improved carbohydrate recognition. rTBL-1 affinity for a broad spectrum of mono- and disaccharides was evaluated by isothermal titration calorimetry (ITC); however, no interaction was detected, corroborating that carbohydrate recognition is highly specific and requires larger ligands for binding. This would explain the differential recognition between healthy and cancer cells by Tepary bean lectins.

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“…In animal models, high doses of isolated legume lectins and raw legume flours have been shown to impair the integrity of the intestinal mucosa by inducing intestinal hyperplasia, altering villus architecture, reducing disaccharidase activity, increasing intestinal permeability and activating the immune system ( Table 1 ) [ 32 , 33 ]. This change in intestinal integrity resulted in compromised nutrient absorption (protein, lipid and vitamin B12) and reduced growth of experimental animals [ 34 , 35 , 36 , 37 ].…”

Section: Lectinsmentioning

confidence: 99%

Is There Such a Thing as “Anti-Nutrients”? A Narrative Review of Perceived Problematic Plant Compounds

2020

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Plant-based diets are associated with reduced risk of lifestyle-induced chronic diseases. The thousands of phytochemicals they contain are implicated in cellular-based mechanisms to promote antioxidant defense and reduce inflammation. While recommendations encourage the intake of fruits and vegetables, most people fall short of their target daily intake. Despite the need to increase plant-food consumption, there have been some concerns raised about whether they are beneficial because of the various ‘anti-nutrient’ compounds they contain. Some of these anti-nutrients that have been called into question included lectins, oxalates, goitrogens, phytoestrogens, phytates, and tannins. As a result, there may be select individuals with specific health conditions who elect to decrease their plant food intake despite potential benefits. The purpose of this narrative review is to examine the science of these ‘anti-nutrients’ and weigh the evidence of whether these compounds pose an actual health threat.

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Effects of intragastrically-administered Tepary bean lectins on digestive and immune organs: Preclinical evaluation

Abstract: Graphical abstract

Cited by 18 publications

References 40 publications

Tepary Bean (Phaseolus acutifolius) Lectins Induce Apoptosis and Cell Arrest in G0/G1 by P53(Ser46) Phosphorylation in Colon Cancer Cells

Tepary Bean (Phaseolus acutifolius) Lectins Induce Apoptosis and Cell Arrest in G0/G1 by P53(Ser46) Phosphorylation in Colon Cancer Cells

Recombinant Lectin from Tepary Bean (Phaseolus acutifolius) with Specific Recognition for Cancer-Associated Glycans: Production, Structural Characterization, and Target Identification

Is There Such a Thing as “Anti-Nutrients”? A Narrative Review of Perceived Problematic Plant Compounds

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