Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and Decidua Basalis

Kusuma, Gina D.; Abumaree, Mohamed; Pertile, Mark D.; Kalionis, Bill

doi:10.1007/978-1-4939-7498-6_19

Cited by 16 publications

(9 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human placenta not only plays a fundamental role in fetal development, nutrition, and immune tolerance [Svensson-Arvelund et al, 2015], but can also be used as MSC source for commercial stem cell banks. MSCs could be isolated from 3 different parts of placenta, named chorionic plate MSCs (CP-MSCs), amniotic membrane MSCs (AM-MSCs), and decidual plate MSCs (DP-MSCs), respectively [Oliveira and Barreto-Filho, 2015;Kusuma et al, 2018]. These 3 types of MSCs possess general characteristics of other tissue-derived MSCs, like umbilical cord-derived MSCs (UC-MSCs), including low immunogenicity, immunoregulation, and multi-lineage differentiation capacity [Diaz-Prado et al, 2010;Nogami et al, 2012;Abumaree et al, 2017;Kim et al, 2018].…”

Section: Introductionmentioning

confidence: 99%

Analysis of Key Distinct Biological Characteristics of Human Placenta-Derived Mesenchymal Stromal Cells and Individual Heterogeneity Attributing to Donors

Tai¹,

Wang²,

Xie³

et al. 2021

Cells Tissues Organs

View full text Add to dashboard Cite

For potential clinical applications in the future, we investigated the distinct biological features of mesenchymal stromal cells (MSCs) derived from different origin areas of human placenta and individual heterogeneity among different donors. Chorionic plate MSCs (CP-MSCs), amniotic membrane MSCs (AM-MSCs), and decidual plate MSCs (DP-MSCs) were isolated from 5 human placentae and were analyzed in terms of main features of MSCs including surface marker profile, growth, differentiation potential, immune regulation capability, and tubulin acetylation (Ac-tubulin). The expression profile of surface markers in the 3 types of MSCs derived from the 5 donors was relatively stable. Heterogeneity was found in growth, differentiation potential, and immune regulation among MSCs according to the different areas of isolation and different donors. CP-MSCs and AM-MSCs derived from the placentae of donors 1–3 had a higher osteogenic differentiation potential than the corresponding DP-MSCs, but those derived from the placentae of donors 4 and 5 had a markedly lower osteogenic differentiation potential than DP-MSCs. All CP-MSCs derived from donors 1–3 had the highest adipogenic differentiation potential, but CP-MSCs derived from donors 4 and 5 did not show strong capability of adipogenic differentiation. CP-MSCs markedly inhibited the proliferation of peripheral blood mononuclear cells (PBMCs) induced by phytohemagglutinin, whereas AM- and DP-MSCs did not. All MSCs decreased the proportion of CD3+/CD8–/IFN-γ+ Th1 and CD3+/CD8–/IL17+ Th17 cells, but increased the proportion of Treg cells in PBMCs, with individual differences among the 5 donors. DP-MSCs from donors 1 and 2 had higher levels of Ac-tubulin compared with CP- and AM-MSCs. However, the levels of Ac-tubulin in AM-MSCs from donors 3 and 5 were higher than those of the other 2 types of MSCs. Our results revealed that there was tissue-specific heterogeneity among the 3 types of MSCs from different origin tissues of placenta and individual heterogeneity among donors. In future, the pre-selected placenta-derived MSCs with specific biological advantages may improve the curative effect of cell therapy in different situations.

show abstract

Section: Introductionmentioning

confidence: 99%

Analysis of Key Distinct Biological Characteristics of Human Placenta-Derived Mesenchymal Stromal Cells and Individual Heterogeneity Attributing to Donors

Tai¹,

Wang²,

Xie³

et al. 2021

Cells Tissues Organs

View full text Add to dashboard Cite

show abstract

“…The morphology of passaged CVCs and AFCs differed, with CVCs being slender with clear boundaries, and the AFCs being flat; both were homogeneous as determined by bright field observation. Actually, CVCs [11] and AFCs [12] were mesenchymal stromal cells (MSCs), which represent a part bio-function of their sources. Here, we check the surface biomarkers of MSCs [13].…”

Section: Discussionmentioning

confidence: 99%

Colchicine causes prenatal cell toxicity and increases tetraploid risk

Wang

Xie

Yan

et al. 2019

BMC Pharmacol Toxicol

View full text Add to dashboard Cite

BackgroundColchicine is a clinical medicine used for relief from gout and familial Mediterranean fever. Because of its toxic effects, intravenous injection of colchicine has been banned, but it is still widely administered orally. We assayed the toxic effects of colchicine in cultured primary chorionic villus cells and amniotic fluid cells to interpret its influence on the placenta and foetus.MethodsBright field record and cell count kit 8 were used to value cell viability. Flow cytometer was used to identify cells markers, cell cycle and cell apoptosis. G-banding was used for karyotype analysis for sample genetic and drug effect evaluation.ResultsChorionic villus cells and amniotic fluid cells were characterized as mesenchymal cells that share most cell surface markers and have a similar response to colchicine. Colchicine did not induce a decline in cell viability at low concentrations but suppressed cell proliferation by arresting the cell cycle in the G2/M phase and increased the risk of tetraploid generation in a small subset of cases.ConclusionsOur study revealed the results of a colchicine-induced toxicity test in prenatal cells and determined the anti-mitotic biologically functional dose and manner of administration that might reduce the risk of tetraploid generation.

show abstract

“…The AFCs and CVCs were harvested at different gestational ages and represent different tissues. Chorionic villus tissue was acquired by biopsy of the placenta at [11][12] weeks of gestation, which was an important period of its growth and maturity [8]. The CVCs were acquired from and represent the placenta.…”

Section: Discussionmentioning

confidence: 99%

Colchicine causes prenatal cell toxicity and increases tetraploid risk

Wang

Xie

Yan

et al. 2019

Preprint

View full text Add to dashboard Cite

Background Colchicine is a clinical medicine used for relief from gout and familial Mediterranean fever. Because of its toxic effects, intravenous injection of colchicine has been banned, but it is still widely administered orally. We assayed the toxic effects of colchicine in cultured primary chorionic villus cells and amniotic fluid cells to interpret its influence on the placenta and foetus. Methods Bright field record and cell count kit 8 were used to value cell viability. Flow cytometer was used to identify cells markers, cell cycle and cell apoptosis. G-banding was used for karyotype analysis for sample genetic and drug effect evaluation. Results Chorionic villus cells and amniotic fluid cells were characterized as mesenchymal cells that share most cell surface markers and have a similar response to colchicine. Colchicine did not induce a decline in cell viability at low concentrations but suppressed cell proliferation by arresting the cell cycle in the G2/M phase and increased the risk of tetraploid generation in a small subset of cases. Conclusions Our study revealed the results of a colchicine-induced toxicity test in prenatal cells and determined the anti-mitotic biologically functional dose and manner of administration that might reduce the risk of tetraploid generation.

show abstract

Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and Decidua Basalis

Cited by 16 publications

References 39 publications

Analysis of Key Distinct Biological Characteristics of Human Placenta-Derived Mesenchymal Stromal Cells and Individual Heterogeneity Attributing to Donors

Analysis of Key Distinct Biological Characteristics of Human Placenta-Derived Mesenchymal Stromal Cells and Individual Heterogeneity Attributing to Donors

Colchicine causes prenatal cell toxicity and increases tetraploid risk

Colchicine causes prenatal cell toxicity and increases tetraploid risk

Contact Info

Product

Resources

About