Cascaded signal amplification via target-triggered formation of aptazyme for sensitive electrochemical detection of ATP

Li, Xia; Yang, Jianmei; Xie, Juan; Jiang, Bingying; Yuan, Ruo; Xiang, Yun

doi:10.1016/j.bios.2017.11.005

Cited by 78 publications

(16 citation statements)

References 39 publications

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“…Third, as a practical application, the evolved HEEFTRA was applied to develop a biosensor with excellent specicity, stability, reproducibility, and regenerability for the ultrasensitive detection of miRNA-21, achieving the assay of miRNA from cancer cell lysates. Given these advantages, the programmable mismatch-fueled HEEFTRA shows great potential as a new generation of DNA signal converter to construct biosensors for sensing analysis and clinical diagnosis, like the detection of nucleic acids, 47 ATP, 48 and proteins, 49 and even for applications in other areas 50 aer some small adjustments of the specic nucleic sequence in it.…”

Section: Discussionmentioning

confidence: 99%

Programmable mismatch-fueled high-efficiency DNA signal converter

et al. 2020

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Section: Discussionmentioning

confidence: 99%

Programmable mismatch-fueled high-efficiency DNA signal converter

et al. 2020

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“…This detection system exhibits a superior or comparative sensitivity to the reported assays but has a significantly shorter detection time (Table S3). − Furthermore, to validate the selectivity of the proposed ATP strategy, several other ATP analogous molecules, such as GTP, CTP, and UTP, were examined as controls. As shown in Figure S8, only ATP could trigger the self-replicating system and form the DNA nanowires, which implied that the strategy we proposed here exhibited a good selectivity.…”

Section: Resultsmentioning

confidence: 99%

Self-Replication-Assisted Rapid Preparation of DNA Nanowires at Room Temperature and Its Biosensing Application

Dai

Dong

et al. 2019

Anal. Chem.

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A rapid room-temperature DNA nanowires preparation strategy on the basis of self-replicating catalyzed hairpin assembly (SRCHA) was reported. In this system, three hairpin probes (P1, P2, and P3) were well-designed and partially hybridize to each other, and two split trigger DNA sequences were integrated into P1 and P3, respectively. When the SRCHA was initiated by the trigger DNA, a series of DNA assembly steps based on the toehold-mediated DNA strand displacement were activated, and the Y shaped DNA (P1−P2− P3) was formed. In that case, the two split trigger DNA sequences will come into close-enough proximity to form the trigger DNA replicas, which can initiate the additional SRCHA reaction cycles for DNA nanowire preparation, and eventually a rapid room-temperature DNA nanowires preparation strategy without need of fuel strands was successfully developed. Furthermore, the prepared DNA nanowires have been used to develop a rapid and signal amplified sensing platform for sensitive adenosine triphosphate (ATP) detection.

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“…With varied signal output platforms, the system signal can be delivered through indicators or coupled with another signal amplification method recovering DNAzyme, NPs, and HCR‐analogous isothermal amplification . Li et al extended the probe containing aptamers and metal ion‐dependent DNAzyme for small molecules . DNAzyme is generated with recognition between the aptamer and target.…”

Section: Cha Based Biosensors For Various Targetsmentioning

confidence: 99%

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Liu

Zhang

Xie

et al. 2019

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Nucleic acids are considered as perfect programmable materials for cascade signal amplification and not merely as genetic information carriers. Among them, catalytic hairpin assembly (CHA), an enzyme‐free, high‐efficiency, and isothermal amplification method, is a typical example. A typical CHA reaction is initiated by single‐stranded analytes, and substrate hairpins are successively opened, resulting in thermodynamically stable duplexes. CHA circuits, which were first proposed in 2008, present dozens of systems today. Through in‐depth research on mechanisms, the CHA circuits have been continuously enriched with diverse reaction systems and improved analytical performance. After a short time, the CHA reaction can realize exponential amplification under isothermal conditions. Under certain conditions, the CHA reaction can even achieve 600 000‐fold signal amplification. Owing to its promising versatility, CHA is able to be applied for analysis of various markers in vitro and in living cells. Also, CHA is integrated with nanomaterials and other molecular biotechnologies to produce diverse readouts. Herein, the varied CHA mechanisms, hairpin designs, and reaction conditions are introduced in detail. Additionally, biosensors based on CHA are presented. Finally, challenges and the outlook of CHA development are considered.

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Cascaded signal amplification via target-triggered formation of aptazyme for sensitive electrochemical detection of ATP

Cited by 78 publications

References 39 publications

Programmable mismatch-fueled high-efficiency DNA signal converter

Programmable mismatch-fueled high-efficiency DNA signal converter

Self-Replication-Assisted Rapid Preparation of DNA Nanowires at Room Temperature and Its Biosensing Application

Applications of Catalytic Hairpin Assembly Reaction in Biosensing

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