Mapping brown adipose tissue based on fat water fraction provided by Z‐spectral imaging

Scotti, Alessandro; Tain, Rong Wen; Li, Weiguo; Gil, Victoria; Liew, Chong Wee; Cai, Kejia

doi:10.1002/jmri.25890

Cited by 14 publications

(26 citation statements)

References 35 publications

(73 reference statements)

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“…Body composition was assessed by magnetic resonance imaging (MRI) scan on gestational day 18. Mice were anesthetized with vaporized isoflurane (1.5%) to reduce respiratory motion artifacts and then imaged on a 3.0‐T MRI system (Magnetom Verio; Siemens, Erlangen, Germany) with an eight‐channel wrist coil as previously described . The volumes of the abdominal visceral WAT (vWAT, containing the perigonadal WAT [pWAT]) and subcutaneous WAT (scWAT, containing the inguinal WAT [iWAT]) were calculated for transverse sections via a manual threshold setting in ImageJ 1.41 (National Institutes of Health, Bethesda, Maryland).…”

Section: Methodsmentioning

confidence: 99%

β3‐Adrenergic Activation Improves Maternal and Offspring Perinatal Outcomes in Diet‐Induced Prepregnancy Obesity in Mice

Zhang

Miao

Chen

et al. 2019

Obesity

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Objective Prepregnancy obesity is an epidemic disorder that seriously threatens both maternal and offspring health. This study investigated the effects of β3‐adrenergic receptor (β3‐AR) activation on the perinatal outcomes in a diet‐induced prepregnancy obese (PPO) murine model. Methods Four‐week‐old female C57BL/6 mice were fed high‐fat diet or chow diet for 16 weeks to yield PPO mice and chow‐fed (CF) lean mice, respectively. After successful mating with CF males, the PPO and CF mice were both randomly divided into vehicle control‐ or CL316,243 (a highly selective β3‐AR agonist)‐treated groups. On gestational day 7, subcutaneous infusion of CL316,243 or saline vehicle (1 mg/kg/d) was provided using osmotic pumps. The perinatal outcomes, adipose tissue morphology, and metabolic and inflammatory markers were examined. Results Chronic β3‐AR agonist infusion induced brown adipose tissue activation and white adipose tissue browning and countered obesity‐induced alterations in lipid profiles, insulin resistance, and systemic and local inflammatory states. Moreover, β3‐AR activation was associated with improved placental perfusion and offspring outcomes. Conclusions Our results provide proof‐of‐principle evidence that pharmacological β3‐AR activation may be of therapeutic potential in preventing prepregnancy‐obesity‐associated adverse maternal and offspring perinatal outcomes.

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Section: Methodsmentioning

confidence: 99%

β3‐Adrenergic Activation Improves Maternal and Offspring Perinatal Outcomes in Diet‐Induced Prepregnancy Obesity in Mice

Zhang

Miao

Chen

et al. 2019

Obesity

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“…Compared to PET and SPECT, MR imaging is a more attractive modality for human studies due to no ionizing-radiation. In recent years, developing MRI methods that can differentiate BAT and WAT has been an increasing interest [127][128][129][130]. The unique structure of BAT with multilocular oil droplets and dense mitochondria and abundant capillaries provides a unique basis for MRI to selectively image BAT via different mechanisms of generating image contrast.…”

Section: Magnetic Resonance Imaging (Mri)mentioning

confidence: 99%

Molecular Imaging of Brown Adipose Tissue Mass

Yang

Zhang

Parhat

et al. 2021

IJMS

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Brown adipose tissue (BAT), a uniquely thermogenic tissue that plays an important role in metabolism and energy expenditure, has recently become a revived target in the fight against metabolic diseases, such as obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD). Different from white adipose tissue (WAT), the brown adipocytes have distinctive features including multilocular lipid droplets, a large number of mitochondria, and a high expression of uncoupling protein-1 (UCP-1), as well as abundant capillarity. These histologic characteristics provide an opportunity to differentiate BAT from WAT using imaging modalities, such as PET/CT, SPECT/CT, MRI, NIRF and Ultrasound. However, most of the reported imaging methods were BAT activation dependent, and the imaging signals could be affected by many factors, including environmental temperatures and the states of the sympathetic nervous system. Accurate BAT mass detection methods that are independent of temperature and hormone levels have the capacity to track the development and changes of BAT throughout the lifetime of mammals, and such methods could be very useful for the investigation of potential BAT-related therapies. In this review, we focus on molecular imaging modalities that can detect and quantify BAT mass. In addition, their detection mechanism and limitations will be discussed as well.

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“…Signal intensity as a function of saturation frequency is then used to obtain a Z-spectrum from which water and fat signal components can be extracted, on a voxel-by-voxel basis. This method was initially tested in excised BAT samples and in vivo in humans (73), and later implemented to measure temperature in a water-fat emulsion phantom (74). With this method, the phase of the MR signal is completely ignored, so there are no phase distortions, and no assumption is made on the relative water-fat frequency, which is known to be greatly affected by microscopic field inhomogeneities in supraclavicular fat (69).…”

Section: Z-spectrum Imaging [Recently Proposed Technique]mentioning

confidence: 99%

Magnetic Resonance Imaging Techniques for Brown Adipose Tissue Detection

Junker

Branca

et al. 2020

Front. Endocrinol.

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Magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) methods can non-invasively assess brown adipose tissue (BAT) structure and function. Recently, MRI and MRS have been proposed as a means to differentiate BAT from white adipose tissue (WAT) and to extract morphological and functional information on BAT inaccessible by other means. Specifically, proton MR (1 H) techniques, such as proton density fat fraction mapping, diffusion imaging, and intermolecular multiple quantum coherence imaging, have been employed to access BAT microstructure; MR thermometry, relaxometry, and MRI and MRS with 31 P, 2 H, 13 C, and 129 Xe have shown to provide complementary information on BAT function. The purpose of the present review is to provide a comprehensive overview of MR imaging and spectroscopy techniques used to detect BAT in rodents and in humans. The present work discusses common challenges of current methods and provides an outlook on possible future directions of using MRI and MRS in BAT studies.

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Mapping brown adipose tissue based on fat water fraction provided by Z‐spectral imaging

Abstract: 1 Technical Efficacy: Stage 1 J. Magn. Reson. Imaging 2018;47:1527-1533.

Cited by 14 publications

References 35 publications

β3‐Adrenergic Activation Improves Maternal and Offspring Perinatal Outcomes in Diet‐Induced Prepregnancy Obesity in Mice

β3‐Adrenergic Activation Improves Maternal and Offspring Perinatal Outcomes in Diet‐Induced Prepregnancy Obesity in Mice

Molecular Imaging of Brown Adipose Tissue Mass

Magnetic Resonance Imaging Techniques for Brown Adipose Tissue Detection

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