Saccharopolyspora aidingensis sp. nov., an actinomycete isolated from a salt lake

Abstract

A novel halophilic actinomycete strain, designated TRM 46074, was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074 was aerobic, Gram-stain-positive and grew optimally with 12 % (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were MK-9(H4), MK-10(H8) and MK-10(H4). The G+C content of the DNA was 70.9 mol%. The major fatty acids were anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TRM 46074 belongs to the genus Saccharopolyspora. Strain TRM 46074 had 16S rRNA gene sequence similarity of 99.6 % with the closest described species, Saccharopolyspora ghardaiensis H53. DNA-DNA hybridization between strain TRM 46074 and Saccharopolysporaghardaiensis H53 showed 58.6 % relatedness. On the basis of the evidence from a polyphasic study, strain TRM 46074 is considered to represent a novel species in the genus Saccharopolyspora, for which the name Saccharopolyspora aidingensis sp. nov. is proposed. The type strain is TRM 46074 (=CCTCC AA 2012014=JCM 30185).

Saccharopolyspora : an underexplored source for bioactive natural products

“…2). They were frequently reported from coastal sediments and from marine organisms (sponges, tunicates) (Pimentel-Elardo et al 2008;Souza et al 2017;Suksaard et al 2018), in addition to extreme marine habitats such as deep-sea sediments and hypersaline lakes (Lv et al 2014;Jiang et al 2016;Xia et al 2017). A number of Saccharopolyspora species have been also reported as endophytic micro-organism in different plants (Qin et al 2008;Qin et al 2010;Vaddavalli et al 2014).…”