Anti-tumor effects of cold atmospheric pressure plasma on vestibular schwannoma demonstrate its feasibility as an intra-operative adjuvant treatment

Yoon, Yeo Jun; Suh, Michelle J.; Lee, Hyun Young; Lee, Hae June; Choi, Eun Ha; Moon, In Seok; Song, Kiwon

doi:10.1016/j.freeradbiomed.2017.11.011

Cited by 18 publications

(18 citation statements)

References 72 publications

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“…There are several promising experimental results leading to the situation that plasma application in cancer therapy is now one of the most attractive research fields in plasma medicine (155). Based on the experimental proof of inactivation of single layers of cancer cells by local plasma treatment (165), first of all a supportive plasma application in combination with surgical tumor resections in cases where large-scale tumor removal is impossible, seems to be realistic (166,167). Moreover, first CAP applications in palliative care in patients with advanced squamous cell carcinoma of head and neck have not only resulted in the intended reduction of microbial load and resulting reduction of typical fetid odor, but in some particular cases also in transient tumor remission (168)(169)(170).…”

Section: Medical Application Of Physical Plasma -Present and Futurementioning

confidence: 99%

Plasma Medicine: A Field of Applied Redox Biology

Schmidt

Bekeschus

et al. 2019

In Vivo

219

153

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Section: Medical Application Of Physical Plasma -Present and Futurementioning

confidence: 99%

Plasma Medicine: A Field of Applied Redox Biology

Schmidt

Bekeschus

et al. 2019

In Vivo

219

153

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“…However, the big challenge for the plasma community is the treatment of non-superficial cancers. One approach is as an intra-operative adjuvant treatment [55]. Another method of delivering "plasma species" to deep tissues could be via plasma-activated liquids.…”

Section: Introductionmentioning

confidence: 99%

Role of Short- and Long-Lived Reactive Species on the Selectivity and Anti-Cancer Action of Plasma Treatment In Vitro

Sklias

Sousa

Girard

2021

Cancers

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(1) Plasma-activated liquids (PAL) have been extensively studied for their anti-cancer properties. Two treatment modalities can be applied to the cells, direct and indirect plasma treatments, which differ by the environment to which the cells are exposed. For direct plasma treatment, the cells covered by a liquid are present during the plasma treatment time (phase I, plasma ON) and the incubation time (phase II, plasma OFF), while for indirect plasma treatment, phase I is cell-free and cells are only exposed to PAL during phase II. The scope of this work was to study these two treatment modalities to bring new insights into the potential use of PAL for cancer treatment. (2) We used two models of head and neck cancer cells, CAL27 and FaDu, and three models of normal cells (1Br3, NHK, and RPE-hTERT). PBS was used as the liquid of interest, and the concentration of plasma-induced H2O2, NO2− and NO3−, as well as pH change, were measured. Cells were exposed to direct plasma treatment, indirect plasma treatment or reconstituted buffer (PBS adjusted with plasma-induced concentrations of H2O2, NO2−, NO3− and pH). Metabolic cell activity, cell viability, lipid peroxidation, intracellular ROS production and caspase 3/7 induction were quantified. (3) If we showed that direct plasma treatment is slightly more efficient than indirect plasma treatment and reconstituted buffer at inducing lipid peroxidation, intracellular increase of ROS and cancer cell death in tumor cells, our data also revealed that reconstituted buffer is equivalent to indirect plasma treatment. In contrast, normal cells are quite insensitive to these two last treatment modalities. However, they are extremely sensitive to direct plasma treatment. Indeed, we found that phase I and phase II act in synergy to trigger cell death in normal cells and are additive concerning tumor cell death. Our data also highlight the presence in plasma-treated PBS of yet unidentified short-lived reactive species that contribute to cell death. (4) In this study, we provide strong evidence that, in vitro, the concentration of RONS (H2O2, NO2− and NO3−) in combination with the acidic pH are the main drivers of plasma-induced PBS toxicity in tumor cells but not in normal cells, which makes ad hoc reconstituted solutions powerful anti-tumor treatments. In marked contrast, direct plasma treatment is deleterious for normal cells in vitro and should be avoided. Based on our results, we discuss the limitations to the use of PAL for cancer treatments.

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“…Cold atmospheric plasma (CAP) is a new technology that has attracted much attention in recent years especially in biomedical applications, such as bacterial disinfection, application of skin diseases, dentistry, cell transfection, wound healing and cancer treatment [3–8]. It is an ionized gas generated by electrical discharges in the atmospheric pressure at room temperature [9]. It has reported that plasma can effectively induce cell death in various types of cancer cells, including colon cancer, melanoma, cervical cancer, glioma, multiple myeloma and so on [10–17].…”

Section: Introductionmentioning

confidence: 99%

Effect of cold atmospheric plasma treatment on the metabolites of human leukemia cells

Ning

et al. 2019

Cancer Cell Int

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Background Acute myeloid leukemia (AML) is a typically fatal malignancy and new drug and treatment need to be developed for a better survival outcome. Cold atmospheric plasma (CAP) is a novel technology, which has been widely applied in biomedicine, especially in various of cancer treatment. However, the changes in cell metabolism after CAP treatment of leukemia cells have been rarely studied. Methods In this study, we investigated the metabolite profiling of plasma treatment on leukemia cells based on Gas Chromatography Tandem Time-of-Flight Mass Spectrometry (GC-TOFMS). Simultaneously, we conducted a series of bioinformatics analysis of metabolites and metabolic pathways with significant differences after basic data analysis. Results 800 signals were detected by GC–TOF mass-spectrometry and then evaluated using PCA and OPLS-DA. All the differential metabolites were listed and the related metabolic pathways were analyzed by KEGG pathway. The results showed that alanine, aspartate and glutamate metabolism had a significant change after plasma treatment. Meanwhile, d -glutamine and d -glutamate metabolism were significantly changed by CAP. Glutaminase activity was decreased after plasma treatment, which might lead to glutamine accumulation and leukemia cells death. Conclusions We found the above two metabolic pathways vulnerable to plasma treatment, which might result in leukemia cells death and might be the cornerstone of further exploration of plasma treatment targets. Electronic supplementary material The online version of this article (10.1186/s12935-019-0856-4) contains supplementary material, which is available to authorized users.

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Anti-tumor effects of cold atmospheric pressure plasma on vestibular schwannoma demonstrate its feasibility as an intra-operative adjuvant treatment

Cited by 18 publications

References 72 publications

Plasma Medicine: A Field of Applied Redox Biology

Plasma Medicine: A Field of Applied Redox Biology

Role of Short- and Long-Lived Reactive Species on the Selectivity and Anti-Cancer Action of Plasma Treatment In Vitro

Effect of cold atmospheric plasma treatment on the metabolites of human leukemia cells

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