“…In agreement with our prediction, the rooting profiles observed in this study were quite similar to those that have been observed in the field, where the majority of the roots (>50%) are located in the upper (e.g., 0-25 cm) soil layer under both control and water stress conditions [soybean (Dwyer et al, 1988;Kirkham et al, 1998;Benjamin and Nielsen, 2006;Ordonez et al, 2018)], maize (Dwyer et al, 1988;Kirkham et al, 1998;Ordonez et al, 2018;Zhang et al, 2018), field pea (Cutforth et al, 2013), canola (Cutforth et al, 2013), wheat (White and Kirkegaard, 2010;Cutforth et al, 2013;Kang et al, 2014;Wang et al, 2014;Guo et al, 2016;Hodgkinson et al, 2017), and barley (Dwyer et al, 1988)]. Growth medium also strongly affected root DM distribution by depth, with a higher fraction of root DM distributed in the deeper soil layers in the 0% FS growth medium treatment.…”