Functional analysis of the global repressor Tup1 for maltose metabolism in Saccharomyces cerevisiae: different roles of the functional domains

Abstract

BackgroundTup1 is a general transcriptional repressor of diverse gene families coordinately controlled by glucose repression, mating type, and other mechanisms in Saccharomyces cerevisiae. Several functional domains of Tup1 have been identified, each of which has differing effects on transcriptional repression. In this study, we aim to investigate the role of Tup1 and its domains in maltose metabolism of industrial baker’s yeast. To this end, a battery of in-frame truncations in the TUP1 gene coding region were performed in the industrial baker’s yeasts with different genetic background, and the maltose metabolism, leavening ability, MAL gene expression levels, and growth characteristics were investigated.ResultsThe results suggest that the TUP1 gene is essential to maltose metabolism in industrial baker’s yeast. Importantly, different domains of Tup1 play different roles in glucose repression and maltose metabolism of industrial baker’s yeast cells. The Ssn6 interaction, N-terminal repression and C-terminal repression domains might play roles in the regulation of MAL transcription by Tup1 for maltose metabolism of baker’s yeast. The WD region lacking the first repeat could influence the regulation of maltose metabolism directly, rather than indirectly through glucose repression.ConclusionsThese findings lay a foundation for the optimization of industrial baker’s yeast strains for accelerated maltose metabolism and facilitate future research on glucose repression in other sugar metabolism.Electronic supplementary materialThe online version of this article (10.1186/s12934-017-0806-6) contains supplementary material, which is available to authorized users.

Corepressors SsnF and RcoA Regulate Development and Aflatoxin B1 Biosynthesis in Aspergillus flavus NRRL 3357

“…Cyc8 (homologous with Ssn6 or SsnF) and Tup1 (homologous with RcoA) are critical transcriptional repressors in CCR. They are involved in nutrient uptake, mating type, and other metabolisms in S. cerevisiae [ 14 ]. In yeast, the recruit proteins Cyc8 and Tup1 are recruited by Mig1, and regulate the target’s expressions by binding to the promoter regions of downstream genes, such as diverse hydrolase genes [ 15 ].…”

Convergence between Regulation of Carbon Utilization and Catabolic Repression in Xanthophyllomyces dendrorhous

“…Notably, the similar profiles of DAPs shared between mutants cyc8 Ϫ/Ϫ and tup1 Ϫ/Ϫ , for instance, showing upregulated glycolysis and gluconeogenesis proteins and downregulated amino acid biosynthesis proteins, suggest opposite and collaborative roles according to the environmental conditions. Indeed, it has been reported previously that the Cyc8-Tup1 complex showed antagonistic and activator effects (26)(27)(28), but it is necessary to elucidate this behavior and its effect on carotenogenesis.…”

Tup1 is critical for transcriptional repression in Quiescence in S. cerevisiae

“…Tup1 plays a role in histone deacetylation and chromatin remodeling in other contexts [ 21 , 28 ]; however, it is unclear whether Tup1 contributes to these processes in quiescence. Tup1 has been identified as a gene that is essential for viability of cells in G0 and is implicated in glucose repression in yeast, but there is no molecular mechanism to explain its role in these processes [ 42 – 46 ]. Here, we demonstrate that Tup1 is essential for repressing target genes in stationary phase by interacting with Rpd3 and Isw2 to generate regions of repressed chromatin.…”