Cloning and Expression of Novel Aminoglycoside Phosphotransferase Genes from Campylobacter and Their Role in the Resistance to Six Aminoglycosides

Zhao, Shaohua; Mukherjee, Sampa; Li, C.; Jones, S. B.; Young, Shenia; McDermott, Patrick F.

doi:10.1128/aac.01682-17

Cited by 11 publications

(7 citation statements)

References 7 publications

(10 reference statements)

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“…Significantly lower TFs (p < 0.05) were observed with erythromycin resistance (<10 −8 ) (Figure 1). For each donor-recipient combination, the TFs to gentamicin resistance were similar overall to those for kanamycin resistance (Figure 1), in support of previous evidence that the gentamicin resistance gene aph(2")-If also mediated resistance to kanamycin [32]. Gentamicin-selected transformants grew readily on MHA with kanamycin (64 µg/mL), and vice versa, kanamycin-selected transformants grew on MHA with gentamicin (4 µg/mL).…”

Section: Differences In Transformation Frequencies Of C Jejuni Sn:cm ...supporting

confidence: 81%

Differences in the Propensity of Different Antimicrobial Resistance Determinants to Be Disseminated via Transformation in Campylobacter jejuni and Campylobacter coli

et al. 2022

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Campylobacter jejuni and Campylobacter coli are leading zoonotic foodborne pathogens, and the drugs of choice for human campylobacteriosis are macrolides (e.g., erythromycin) and fluoroquinolones. C. jejuni and C. coli are naturally competent for transformation via naked DNA uptake, but potential differences in transformation frequency (TF) for different antimicrobial resistance (AMR) markers remain poorly understood. We determined TFs for resistance to different antibiotics using as recipient a derivative of C. jejuni NCTC 11168 (strain SN:CM) with donor DNA from multidrug-resistant C. jejuni or C. coli. TF for nalidixic acid resistance ranked significantly highest (~1.4 × 10−3), followed by resistance to streptomycin and gentamicin. Tetracycline resistance via chromosomal tet(O) was less commonly transferred (~7.6 × 10−7), while transformation to erythromycin resistance was rare (≤4.7 × 10−8). We also determined TFs with the contemporary poultry-derived strains C. jejuni FSIS 11810577 and C. coli FSIS 1710488 as recipients. TFs to nalidixic acid and streptomycin resistance remained the highest (~7 × 10−4). However, TF for gentamicin resistance was remarkably low in certain recipient–donor combinations, while average TF for erythromycin resistance was noticeably higher (~3 × 10−6) than with SN:CM. Findings from this experimental model provide insights into factors that may impact transformation-mediated transfer of AMR leading to AMR dissemination in the agricultural ecosystem.

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Section: Differences In Transformation Frequencies Of C Jejuni Sn:cm ...supporting

confidence: 81%

Differences in the Propensity of Different Antimicrobial Resistance Determinants to Be Disseminated via Transformation in Campylobacter jejuni and Campylobacter coli

et al. 2022

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“…In addition, the antibiotic resistant genotypes were well-corresponding to the ST types (Figures 1, 2). The aminoglycoside-resistant genes ant(6)-Ia, aph(3')-III, aph(2")-If in C. jejuni and C. coli were frequently reported from other studies, which were related to the resistance to aminoglycoside (33,34). All isolates contained the catA gene were associated with the resistance to chloramphenicol, which is relatively high compared to other studies.…”

Section: Discussionmentioning

confidence: 72%

Characterization and Prevalence of Campylobacter spp. From Broiler Chicken Rearing Period to the Slaughtering Process in Eastern China

et al. 2020

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Campylobacter is one of the most important foodborne pathogens worldwide, and poultry is regarded as the main reservoir of Campylobacter. The contamination of Campylobacter in broiler chickens at the farm level is closely related to the transmission of Campylobacter in the poultry production chain. This study identified 464 Campylobacter isolates from 1,534 samples from broiler rearing period and slaughtering process including 233 Campylobacter jejuni isolates and 231 Campylobacter coli isolates. We have observed a dynamic distribution of Campylobacter during broiler chicken production, that 66.3% of Campylobacter isolates were C. jejuni during broiler rearing period, while C. coli occupied 60.4% of Campylobacter isolates during the broiler slaughtering process. A tag-label method allowed us to track the dynamic of Campylobacter in each broiler chicken from 31-day age at rearing to the partition step in the slaughterhouse. At the 31-day during rearing, 150 broiler chicken were labeled, and was tracked for Campylobacter positive from rearing period to slaughtering process. Among the labeled broiler, 11 of the tracking broiler samples were able to detect Campylobacter from rearing period to slaughtering. All Campylobacter isolates from the 11 tracking samples were sequenced and analyzed. C. jejuni isolates were divided into four STs and C. coli isolates were divided into six STs. Isolates with identical core genome were observed from the same tag-labeled samples at different stages indicating a vertical transmission of Campylobacter in the early broiler meat production. Meanwhile, the core genome analysis elucidated the cross-contamination of Campylobacter during the rearing period and the slaughtering process. The virulotyping analysis revealed that all C. jejuni isolates shared the same virulotypes, while C. coli isolates were divided into three different virulotypes. The antimicrobial resistance gene analysis demonstrated that all Campylobacter isolates contained at least two antibiotic resistance genes (ARGs), Tang et al. Campylobacter From Broiler in China and the ARG profiles were well-corresponding to each ST type. Our study observed a high prevalence of Campylobacter during the early chicken meat production, and further studies will be needed to investigate the diversity and transmission of Campylobacter in the poultry production chain.

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“…In Belgium, gentamicin resistance in poultry isolates has increased from almost being nonexistent in 2004 to approximately 20% by 2009 (11). Multiple phosphotransferase ( aph ) genes are commonly associated with aminoglycoside resistance, and many of them have been identified as transferable (13, 14, 51).…”

Section: Discussionmentioning

confidence: 99%

Antibiotic Resistance of Campylobacter Species in a Pediatric Cohort Study

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Colston

Olórtegui

et al. 2019

Antimicrob Agents Chemother

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Cloning and Expression of Novel Aminoglycoside Phosphotransferase Genes from Campylobacter and Their Role in the Resistance to Six Aminoglycosides

Cited by 11 publications

References 7 publications

Differences in the Propensity of Different Antimicrobial Resistance Determinants to Be Disseminated via Transformation in Campylobacter jejuni and Campylobacter coli

Differences in the Propensity of Different Antimicrobial Resistance Determinants to Be Disseminated via Transformation in Campylobacter jejuni and Campylobacter coli

Characterization and Prevalence of Campylobacter spp. From Broiler Chicken Rearing Period to the Slaughtering Process in Eastern China

Antibiotic Resistance of Campylobacter Species in a Pediatric Cohort Study

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