Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene

Wang, Jiashun; Li, Yi; Chen, Jia; Dai, Hua; Deng, Hui; Li, Ying; Zhi-xuan, Liang; Huang, Jinhai

doi:10.1016/j.bjm.2017.09.001

Cited by 24 publications

(24 citation statements)

References 46 publications

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“…By using gold nanoparticles (AuNPs) specifically interacting with the labeled isothermal amplification products, colored signals are observed semi-quantitatively with the naked eye on LFS (Yang et al, 2013). Promising results from the RPA-LFS combined method have been reported for detection of Staphylococcus aureus, Salmonella enteritidis, and L. monocytogenes (Olsen et al, 1992;Lee et al, 2011;Gao et al, 2016;Wang et al, 2017;Wu et al, 2017;Du et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Rapid and Specific Detection of Listeria monocytogenes With an Isothermal Amplification and Lateral Flow Strip Combined Method That Eliminates False-Positive Signals From Primer–Dimers

Wang¹,

Zhao

Si³

et al. 2020

Front. Microbiol.

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Listeria monocytogenes is an important foodborne pathogenic bacterium that is explicitly threatening public health and food safety. Rapid, simple, and sensitive detection methods for this pathogen are of urgent need for the increasing on-site testing demands. Application of the isothermal recombinase polymerase amplification (RPA) and the lateral flow strip (LFS) in the detection is promising for fast speed, high sensitivity, and little dependency on equipment and trained personnel. However, the simplicity comes with an intrinsic and non-negligible risk, the false-positive signals from primer-dimers. In this study, an improved RPA-LFS system was established for detection of L. monocytogenes that eliminated false-positive signals from primerdimers. Primer candidates were carefully selected from the entire L. monocytogenes genome sequence and rigorously screened for specific amplifications in PCR and RPA reactions. For the optimal primer pairs, probes that matched the targeted fragment sequences, although had the smallest chance to form cross-dimers with the primers, were designed and screened. The intelligent use of the probe successfully linked the positive signal to the actual amplification product. This RPA-LFS system was highly specific to L. monocytogenes and was able to detect as low as 1 colony-forming unit of the bacterium per reaction (50 µl) without DNA purification, or 100 fg of the genomic DNA/50 µl. The amplification could be conducted under the temperature between 37 and 42 • C, and the whole detection finished within 25 min. Test of artificially contaminated milk gave 100% accuracy of detection without purification of the samples. Various food samples spiked with 10 colony-forming unit of L. monocytogenes per 25 g or 25 ml were successfully detected after an enrichment time period of 6 h. The RPA-LFS system established in this study is a rapid, simple, and specific detection method for L. monocytogenes that has eliminated false-positive results from primer-dimers. In

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Section: Introductionmentioning

confidence: 99%

Rapid and Specific Detection of Listeria monocytogenes With an Isothermal Amplification and Lateral Flow Strip Combined Method That Eliminates False-Positive Signals From Primer–Dimers

Wang¹,

Zhao

Si³

et al. 2020

Front. Microbiol.

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“…Com relação à presença de CT nas águas brutas, a legislação (Brasil, 2017), estabelece ausência, devido estes também são potencialmente organismos de transmissão de doenças em humanos. Incluso no grupo de CT está, por exemplo, espécies do gênero Salmonella, extremamente patogênicas e potencialmente formadoras de biofilmes, sendo agentes de doenças vinculadas a alimentos, em escala mundial (Wang et al, 2018). No Brasil, é o principal enteropatógeno causador de surtos relacionados ao consumo de água e alimentos, no período de 2000 a 2017 .…”

Section: Resultsunclassified

Avaliação bacteriológica da água de consumo em serviços de alimentação

Macedo

Shinohara

Oliveira

2020

RSD

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A água é um composto essencial à manutenção da vida, fundamental para atender necessidades humanas e atividades profissionais. Em serviços de alimentação, a água é utilizada em diversas atividades, com destaque para a higienização durante as etapas produtivas. Entretanto, se a sua qualidade estiver comprometida, devido à presença de microrganismos patogênicos, por exemplo, pode se tornar um vetor para transmissão de doenças de veiculação hídrica. O objetivo deste trabalho foi avaliar a presença de coliformes totais (CT) e Escherichia coli na água de consumo utilizada na produção em trinta unidades de serviços de alimentação na cidade do Recife/PE. A metodologia de identificação para CT e E. coli foi o teste de presença/ausência, utilizando substrato cromogênico X-GAL e fluorogênico MUG. Foi detectada a presença de CT em trinta e duas amostras, das quais 6 também foram positivas para E. coli. Conclui-se que do total de trinta estabelecimentos investigados, cerca de 54% utilizavam água na produção de alimentos que não atendiam o padrão microbiológico preconizado pela legislação brasileira, necessitando de medidas corretivas e educativas para garantir que não haja agravos à saúde.

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“…Serotyping has wide acceptance as a method to differentiate Salmonella strains, and it is an important tool in public health [ 67 ]. Immunology-based methods involving antigen-antibody bindings have been widely used for the detection of food-borne pathogens [ 85 ]. The immunology-based assays include ELISA, latex agglutination tests, immunodiffusion, and immunochromatography (dipstick).…”

Section: Food-borne Zoonotic Bacterial Pathogensmentioning

confidence: 99%

Review on Major Food-Borne Zoonotic Bacterial Pathogens

Abebe

Gugsa

Ahmed

2020

Journal of Tropical Medicine

316

203

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Food-borne microorganisms are major pathogens affecting food safety and cause human illness worldwide as a result of consumption of foodstuff, mainly animal products contaminated with vegetative pathogens or their toxins. Most of these microbes have zoonotic importance resulting in significant impact on both public health and economic sectors. Bacteria are the causative agents of two-thirds of human food-borne diseases worldwide with high burden in developing countries. Hence, the objectives of this review paper are to highlight the background of food-borne bacterial pathogens and to review common major food-borne zoonotic bacterial pathogens. Food animals are the major reservoirs of many food-borne zoonotic bacterial pathogens, and food products of animal origin are the main vehicles of transmission. Meat, dairy products, and eggs are the main ways by which people are exposed to zoonotic bacteria. S. aureus, Salmonella species, Campylobacter species, L. monocytogenes, and E. coli are the major zoonotic bacterial pathogens which are the causative agents of food-borne illness and death in the world associated with consumption of contaminated animal products. Production of toxins and structural virulent factors are responsible for the pathogenesis of these bacteria. These major zoonotic bacteria cause human infections which are characterized mainly by gastrointestinal symptoms including nausea, vomiting, diarrhea, abdominal cramps, and other agent-specific symptoms. Some bacteria may cause severe complications. Conventional (culturing), serological, and molecular techniques are important for detection of these common zoonotic bacteria and their toxins in food. Good hygiene, GMP, sanitation in operating procedures, and implementation of standardized HACCP and pasteurization procedures are effective methods for the control and prevention. Currently, the emergence of multidrug-resistant zoonotic bacteria associated with consumption of contaminated animal products is a great concern for the public health, and there should be coordinated surveillance and monitoring system for food-borne zoonotic bacterial pathogens particularly in developing countries including Ethiopia.

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Rapid detection of food-borne Salmonella contamination using IMBs-qPCR method based on pagC gene

Cited by 24 publications

References 46 publications

Rapid and Specific Detection of Listeria monocytogenes With an Isothermal Amplification and Lateral Flow Strip Combined Method That Eliminates False-Positive Signals From Primer–Dimers

Rapid and Specific Detection of Listeria monocytogenes With an Isothermal Amplification and Lateral Flow Strip Combined Method That Eliminates False-Positive Signals From Primer–Dimers

Avaliação bacteriológica da água de consumo em serviços de alimentação

Review on Major Food-Borne Zoonotic Bacterial Pathogens

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