S -nitroso- and nitro- proteomes in the olive ( Olea europaea L.) pollen. Predictive versus experimental data by nano-LC-MS

Abstract

The data presented here are related to the research article entitled “Generation of nitric oxide by olive (Olea europaea L.) pollen during in vitro germination and assessment of the S-nitroso- and nitro-proteomes by computational predictive methods” doi:10.1016/j.niox.2017.06.005 (Jimenez-Quesada et al., 2017) [1]. Predicted cysteine S-nitrosylation and Tyr-nitration sites in proteins derived from a de novo assembled and annotated pollen transcriptome from olive tree (Olea europaea L.) were obtained after using well-established predictive tools in silico. Predictions were performed using both default and highly restrictive thresholds. Numerous gene products identified with these characteristics are listed here. An experimental validation of the data, consisting in nano-LC-MS (Liquid Chromatography-Mass Spectrometry) determination of olive pollen proteins after immunoprecipitation with antibodies to anti-S-nitrosoCys and anti-3-NT (NitroTyrosine) allowed identification of numerous proteins subjected to these two post-translational modifications, which are listed here together with information regarding their cross-presence among the predictions.

Generation of Superoxide by OeRbohH, a NADPH Oxidase Activity During Olive (Olea europaea L.) Pollen Development and Germination

“…As mentioned before, the production of superoxide by OeRbohH and other RbohHs has been demonstrated to be modulated by multiple factors including Ca 2+ signaling, acidic signaling phospholipids, and small GTP-binding proteins from the Rac/Rop family (Potocky et al, 2012). Moreover, we have evidence of OeRbohH regulation by S -nitrosylation events, which may exert changes in its function (Jimenez-Quesada et al, 2017a; Jimenez-Quesada et al, 2017b). Such PTM is highly dependent on the levels of NO (GSNO) (Corpas et al, 2013), which are critically acting on olive pollen germination physiology (Jimenez-Quesada et al, 2017a; Jimenez-Quesada et al, 2017b).…”

“…Moreover, we have evidence of OeRbohH regulation by S -nitrosylation events, which may exert changes in its function (Jimenez-Quesada et al, 2017a; Jimenez-Quesada et al, 2017b). Such PTM is highly dependent on the levels of NO (GSNO) (Corpas et al, 2013), which are critically acting on olive pollen germination physiology (Jimenez-Quesada et al, 2017a; Jimenez-Quesada et al, 2017b).…”

Stem cells and pain

“…SC can also increase the level of cytokines capable of decreasing nociception such as IL-4[ 46 ] and IL-10[ 53 , 54 ]. On the other hand, treatment with SC can increase the level of molecules indirectly linked to the promotion of hyperalgesia, such as NO (has a dual role in pain)[ 45 , 55 ], PGE 2 [ 46 , 56 ] and IDO[ 47 , 57 ].…”