Cold modulated nuclear S-nitrosoproteome analysis indicates redox modulation of novel Brassicaceae specific, myrosinase and napin in Brassica juncea

Proteomic Analysis of S-Nitrosation Sites During Somatic Embryogenesis in Brazilian Pine, Araucaria angustifolia (Bertol.) Kuntze

“…In leaves of Camellia sinensis , two subunits of MDH were S -nitrosated, suggesting a role of this PTM in regulating enzyme activities associated with pyruvate metabolism ( Qiu et al, 2019 ). NO donors inhibit the activity of MDH, and exposition to abiotic stresses affects its S -nitrosation pattern ( Jain et al, 2018 ; Sehrawat et al, 2019 ). L-ascorbate oxidases (AOs) are glycosylated blue multicopper oxidases of the cupredoxin superfamily responsible for ascorbic acid oxidation with the production of monodehydroascorbate (MDHA) and water.…”

Understanding the Proteomes of Plant Development and Stress Responses in Brassica Crops

“…108 . 109 Proteomic analysis in B. juncea leaf cuticle identified various PTMs, wherein N-glycosylation was observed mostly in the Nterminal of proteins that were related to lipid metabolism and included ESM1, PLAT domain containing protein, and GDSL esterase/lipase. 161 N-Terminal proteolysis, phosphorylation and S-nitrosylation were observed in different classes of proteins that included carbohydrate and lipid metabolism, proteases, stress, and related pathways.…”

“…In B. juncea , various molecules like nitric oxide, napin, and myrosinase-associated proteins have been reported to alleviate low temperature stress. , In another Brassica species ( B. rapa ), a quantitative proteome analysis by Xu et al, in leaves led to the identification of DAPs. These proteins allowed the B. rapa cultivar Longyou 7 to survive the cold conditions by regulating the pentose phosphate pathway, TCA cycle, glyoxylate, and dicarboxylate, with an overall effect of decreased energy metabolism and photosynthesis, the causal mechanisms leading to a balanced synthesis and utilization of sugar.…”

Nitric oxide regulation of temperature acclimation: a molecular genetic perspective

“…Exogenous NO treatment leads to a reduction in the level of malondialdehyde (MDA), whilst chlorophyll content is increased and SUPEROXIDE DISMUTASE (SOD) and ASCORBATE PEROXIDASE (APX) activities are promoted. In addition, it has been found that the source of NO production during the cold response originates mainly from the activity of NITRATE REDUCTASE (NR) ( Zhao et al , 2009 ; Cantrel et al , 2011 ; Lv et al , 2017 ; Sehrawat et al , 2019 ). It has been reported that cold acclimation stimulates NR activity and transcription of NITRATE REDUCTASE 1 ( NIA1 ), thus increasing the NO content, a result that is supported by a lower degree of freeze tolerance being observed in the Arabidopsis nia1 nia2 mutant ( Zhao et al , 2009 ).…”

“…Although studies have shown that nitrosated proteins are clearly accumulated, only about 30% of those identified are consistent between the different analyses reported, which emphasizes the highly dynamic nature of NO regulation. Other recent research has also drawn attention to the subcellular changes that occur when NO is attached to cysteine residues, suggesting the existence of nitrosation-mediated nuclear trafficking that has an impact on cellular metabolism and redox status ( Sehrawat et al , 2019 ). These findings are also supported by results obtained in bermudagrass that show that NO has a crucial role in maintaining cell membrane stability, in antioxidant responses, and in PSII recovery, with temperature changes involving interconnections between H 2 O 2 and NO during the plant responses ( Fan et al , 2015 ).…”