A New Cell Separation Method Based on Antibody-Immobilized Nanoneedle Arrays for the Detection of Intracellular Markers

Kawamura, Ryuzo; Miyazaki, Minami; Shimizu, Keita; Matsumoto, Yuta; Silberberg, Yaron; Sathuluri, Ramachandra Rao; Iijima, Masumi; Kuroda, Shinya; Iwata, Futoshi; Kobayashi, Takeshi; Nakamura, Chikashi

doi:10.1021/acs.nanolett.7b03918

Cited by 25 publications

(9 citation statements)

References 40 publications

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“…Kawamura et al have demonstrated a unique form of cell capture and sorting using antibody‐functionalized silicon nanoneedle arrays . They interfaced cocultures of two different cell types using nanoneedle arrays.…”

Section: Biochemical Sensingmentioning

confidence: 99%

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

et al. 2020

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Materials patterned with high‐aspect‐ratio nanostructures have features on similar length scales to cellular components. These surfaces are an extreme topography on the cellular level and have become useful tools for perturbing and sensing the cellular environment. Motivation comes from the ability of high‐aspect‐ratio nanostructures to deliver cargoes into cells and tissues, access the intracellular environment, and control cell behavior. These structures directly perturb cells' ability to sense and respond to external forces, influencing cell fate, and enabling new mechanistic studies. Through careful design of their nanoscale structure, these systems act as biological metamaterials, eliciting unusual biological responses. While predominantly used to interface eukaryotic cells, there is growing interest in nonanimal and prokaryotic cell interfacing. Both experimental and theoretical studies have attempted to develop a mechanistic understanding for the observed behaviors, predominantly focusing on the cell–nanostructure interface. This review considers how high‐aspect‐ratio nanostructured surfaces are used to both stimulate and sense biological systems.

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Section: Biochemical Sensingmentioning

confidence: 99%

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

et al. 2020

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“…In addition to advancements in delivery, several sampling devices equipped with nanoneedle arrays were established for monitoring intracellular activity under the assistance of steerable forces supported by simple pressing or micromanipulators. [ 53,150 ] Using their diamond nanoneedle platform, Wang et al accomplished in situ probing of specific intracellular signaling elements involving in cellular innate immunity after the stimulator of interferon genes (STING) pathway was activated by nanoneedle‐mediated delivery of double‐stranded DNAs (Figure 7c). [ 149 ] After the aptamer‐modified diamond nanoneedles were inserted into the cytoplasmic domain by centrifugation, the surface bound aptamer molecules could specifically recognize the target biomolecules of interest (such as the transcription factor NF‐κB) in the cytosol.…”

Section: Assisted Penetrationmentioning

confidence: 99%

Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

He¹,

Hu²,

et al. 2020

Adv Funct Materials

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Establishing techniques to efficiently and nondestructively access the intracellular milieu is essential for many biomedical and scientific applications, ranging from drug delivery, to electrical recording, to biochemical detection. Cell penetration using nanoneedle arrays is currently a research focus area because it not only meets the increasing therapeutic demands of cell modifications and genome editing, but also provides an ideal platform for tracking long-term intracellular information. Although the precise mechanism driving membrane penetration by nanoneedle arrays is still unclear, the low cytotoxicity, wide range of delivered materials, diverse cell type targets, and simple material structures of nanoneedle arrays make these splendid platforms for cell access. Here, the recent progress in this field is reviewed by examining device architectures and discussing mechanisms for nanoneedle penetration, and the major studies demonstrating the most general applicability of nanoneedle arrays, typical methodologies to access the intracellular environment using nanoneedles with spontaneous or assisted penetration modes, as well as biosafety aspects are presented. This review should be valuable for deeply understanding the materials fabrication principles, device designs, cell penetration methodologies, biosafety aspects, and application strategies of nanoneedle array-based systems that are of crucial importance for the development of future practical biomedical platforms.

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“…By a similar strategy, antibody-functionalized nanoneedle array was fabricated to target individual cells, and separate them from a mixed population of cells on a substrate (Kawamura et al, 2017). The nanoneedle array comprised of 10,000 nanoneedles was fabricated using top-down MEMS (Micro-Electro-Mechanical System) technique with each nanoneedle <200 nm diameter and more than 20 µm long.…”

Section: Nanoneedle For Single-cell Samplingmentioning

confidence: 99%

The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling

Jia

Chen

2020

Front. Chem.

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The research in endogenous biomolecules from a single cell has grown rapidly in recent years since it is critical for dissecting and scrutinizing the complexity of heterogeneous tissues, especially under pathological conditions, and it is also of key importance to understand the biological processes and cellular responses to various perturbations without the limitation of population averaging. Although conventional techniques, such as micromanipulation or cell sorting methods, are already used along with subsequent molecular examinations, it remains a big challenge to develop new approaches to manipulate and directly extract small quantities of cytosol from single living cells. In this sense, nanostructure or nanomaterial may play a critical role in overcoming these challenges in cellular manipulation and extraction of very small quantities of cells, and provide a powerful alternative to conventional techniques. Since the nanostructures or nanomaterial could build channels between intracellular and extracellular components across cell membrane, through which cytosol could be pumped out and transferred to downstream analyses. In this review, we will first brief the traditional methods for single cell analyses, and then shift our focus to some most promising methods for single-cell sampling with nanostructures, such as glass nanopipette, nanostraw, carbon nanotube probes and other nanomaterial. In this context, particular attentions will be paid to their principles, preparations, operations, superiorities and drawbacks, and meanwhile the great potential of nano-materials for single-cell sampling will also be highlighted and prospected.

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A New Cell Separation Method Based on Antibody-Immobilized Nanoneedle Arrays for the Detection of Intracellular Markers

Cited by 25 publications

References 40 publications

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

High‐Aspect‐Ratio Nanostructured Surfaces as Biological Metamaterials

Nanoneedle Platforms: The Many Ways to Pierce the Cell Membrane

The Most Recent Advances in the Application of Nano-Structures/Nano-Materials for Single-Cell Sampling

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