Tunable Electroosmosis-Based Femto-Liter Pipette: A Promising Tool toward Living-Cell Surgery

Chen, Apeng; Lynch, Kyle B.; Ren, Jiangtao; Jia, Zhijian; Yu, Yihua; Lü, Jianmin; Liu, Shaorong

doi:10.1021/acs.analchem.7b02132

Cited by 19 publications

(27 citation statements)

References 38 publications

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“…The first category of nanopipettes utilize a combination of electroosmotic flows and electrophoresis to extract cellular content (or to deliver cargo into) a cell. [287][288][289][290] To extract cellular contents, the nanopipette is first filled with an electrolyte solution (e.g. ammonium acetate solution).…”

Section: Transport Driven By Electric Field 511 Nanopipettesmentioning

confidence: 99%

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Microfluidic Surgery in Single Cells and Multicellular Systems

Zhang¹,

Nadkarni²,

Paul³

et al. 2021

Preprint

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Microscale surgery on single cells and small organisms have enabled major advances in fundamental biology and in engineering biological systems. Examples of applications range from wound healing and regeneration studies to the generation of hybridoma to produce monoclonal antibodies. Even today, these surgical operations are often performed manually, but they are labor-intensive and lack reproducibility. Microfluidics has emerged as a powerful technology to control and manipulate cells and multicellular systems at the micro- and nanoscale with high precision. Here, we review the physical and chemical mechanisms of microscale surgery, and the corresponding design principles, applications, and implementations in microfluidic systems. We consider four types of surgical operations: 1) Sectioning, which splits a biological entity into multiple parts, 2) ablation, which destroys part of an entity, 3) biopsy, which extracts materials from within a living cell, and 4) fusion, which joins multiple entities into one. For each type of surgery, we summarize the motivating applications and the microfluidic devices developed. Throughout the review, we highlight existing challenges and opportunities. We hope that this review will inspire scientists and engineers to continue to explore and improve microfluidic surgical methods.

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Section: Transport Driven By Electric Field 511 Nanopipettesmentioning

confidence: 99%

“…ammonium acetate solution). 287,290 An electrode is then inserted into the nanopipette and immersed into the electrolyte solution. The counter electrode is inserted in the culture medium.…”

Section: Transport Driven By Electric Field 511 Nanopipettesmentioning

confidence: 99%

Microfluidic Surgery in Single Cells and Multicellular Systems

Zhang¹,

Nadkarni²,

Paul³

et al. 2021

Preprint

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“…Moreover, it has the characteristics of continuously and accurately transporting micro-liter and nano-liter fluids, no moving parts, no pulsation, no mechanical wear and avoiding micro-leakage from valves and conveying process. EOP has been applied in flow injection analysis (FIA) 1 , micro-, nano liquid chromatography (HPLC) separation [2][3][4] , femto-pipette 5,6 , electroosmotic droplet switch 7,8 and micro-cooling systems 9 .…”

Section: Introductionmentioning

confidence: 99%

A Performance-enhanced Electroosmotic Pump with Track-etched Polycarbonate Membrane by Allylhydridopolycarbosilane Coating

et al. 2020

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Nanochannel plastic membranes are excellent materials for electroosmotic pump (EOP) elements owing to their surface charge properties, flexibility and cost-effectiveness. However, the surface charge properties of plastics are inferior to those of silicate-based materials. This paper reports a performance-enhanced EOP equipped with a glassified track-etch polycarbonate membrane (PC), which has a nanochannel surface covered by allylhydridopolycarbosilane (AHPCS). The effects of applied voltage, pH and membrane pore size on the electroosmotic flow velocity, along with comparative study of the EOP with coated and pure membranes were investigated. It was found that when low DC voltage (1040 V) was applied at both ends of the pump, the magnitude of the electroosmotic flow was linearly proportional to the voltage when the pore sizes of membrane were below 600-nm. Higher the flow rate obtained with larger pore size membrane. Compared with the uncoated film, the coated one showed faster electroosmosis velocity, with higher stability under the same conditions. For pH 10.0 buffer solution, a flow rate of 89.13 μL/min obtained in the modified membrane based EOP with excellent repeatability and durability, while the flow rate was only 37.89 μL/min in the bare PC membrane under 20 V.In order to demonstrate the performance of the developed EOP, the EOP was used to a microcomplexometric titration to determine actual tap water hardness. The measured results were considerably consistent with a conventional complexometric titration. The EOP with an AHPCS-coated plastic membrane expanded the application ranges to harsh condition solutions like high concentration acids or bases.

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“…The reason for this is that in EOPs the average flow velocity is independent of the channel diameter, 11,12 unlike pressure-driven flow, where the average flow velocity decreases with the square of the channel diameter. EOP-based nanoporous membranes have been applied in various methods of analytical and physical chemistry, such as: liquidchromatography separation, 13 use of femto-liter pipettes, 14 micro-energy systems, 15 and micro-nanofluidic microchips. 16 A common challenge facing the field is that in many situations involving EOPs, there is a tendency for the microfluidic device to become clogged with aggregates of CPs.…”

Section: Introductionmentioning

confidence: 99%