Metabolomics research of medicinal plants

Duan, Lixin; Dai, Yuntao; Sun, Chao; Chen, Shilin

doi:10.4268/cjcmm20162202

Cited by 3 publications

(2 citation statements)

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“…Non-targeted metabolomics has been applied to investigate the differential metabolites and analyze the enriched pathways, especially in studies on the discovery of metabolic markers. Due to high throughput characteristics and a wide coverage of metabolites (Duan et al, 2016), all cellular metabolites can be detected without bias, more comprehensively reflecting the overall metabolic state of cells (Patti et al, 2012). Non-targeted metabolomics LC-MS was used to analyze the metabolic profiles in wild type and BcWRKY1-OX1 transgenic Arabidopsis thaliana.…”

Section: Discussionmentioning

confidence: 99%

Expression and Functional Study of BcWRKY1 in Baphicacanthus cusia (Nees) Bremek

et al. 2022

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Baphicacanthus cusia (Nees) Bremek (B. cusia) is an important medicinal plant. Its effective substances including indigo and indirubin are metabolites in indoleacetate metabolic pathway. Based on a previous transcriptome sequencing analysis, a WRKY transcription factor, BcWRKY1, in B. cusia was identified, showing significant correlation with effective substances from B. cusia. In this study, BcWRKY1 was cloned by reverse transcription-polymerase chain reaction (RT-PCR). Further analysis showed that the BcWRKY1 gene was 916 bp in length, containing three exons and two introns. The open reading frame (ORF) of BcWRKY1 was 534 bp in length and encoded a WRKY domain-containing protein with 177 amino acids residues. Subcellular localization showed that BcWRKY1 protein was mainly localized in the nucleus. It could bind to the W-box motif and its role in transcriptional activation was confirmed in yeast. The function of BcWRKY1 was investigated by overexpressing BcWRKY1 in Arabidopsis thaliana. Metabolic profiles in wild type and BcWRKY1-OX1 transgenic Arabidopsis thaliana were analyzed with LC-MS. Results showed that the metabolic profile was significantly changed in BcWRKY1-OX1 transgenic Arabidopsis thaliana compared with wild type. Furthermore, indole-related metabolites were significantly increased in BcWRKY1-OX1 transgenic Arabidopsis thaliana, and the metabolic pathway analysis showed that flavonoid biosynthesis was significantly enriched. Overexpression of BcWRKY1 significantly changed flavonoid and indole metabolism and indole-related metabolites were significantly upregulated. We postulated that the BcWRKY1 transcription factor might be involved in the regulation of effective substances metabolism in B. cusia.

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Section: Discussionmentioning

confidence: 99%

Expression and Functional Study of BcWRKY1 in Baphicacanthus cusia (Nees) Bremek

et al. 2022

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“…The metabolites of medicinal plants are the active components of Chinese medicines that might play a therapeutic role and determine the quality of medicinal materials [ 3 , 4 ]. Different cultivation areas, habitats and production methods will have different effects on plant metabolites and the quality of medicinal materials [ 5 , 6 ]. Therefore, when adopting wild sources of medicines for cultivation, the question of whether the quality of the cultivated materials can be guaranteed needs scientific verification.…”

Section: Introductionmentioning

confidence: 99%

Metabolomic Analysis Reveals the Metabolic Diversity of Wild and Cultivated Stellaria Radix (Stellaria dichotoma L. var. lanceolata Bge.)

Wang

Feng

et al. 2023

Plants

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Stellaria Radix, called Yinchaihu in Chinese, is a traditional Chinese medicine, which is obtained from the dried roots of Stellaria dichotoma L. var. lanceolata Bge. Cultivated yinchaihu (YCH) has become a main source of production to alleviate the shortage of wild plant resources, but it is not clear whether the metabolites of YCH change with the mode of production. In this study, the contents of methanol extracts, total sterols and total flavonoids in wild and cultivated YCH are compared. The metabolites were analyzed by ultra-high performance liquid chromatography–tandem time-of-flight mass spectrometry. The content of methanol extracts of the wild and cultivated YCH all exceeded the standard content of the Chinese Pharmacopoeia. However, the contents of total sterols and total flavonoids in the wild YCH were significantly higher than those in the cultivated YCH. In total, 1586 metabolites were identified by mass spectrometry, and 97 were significantly different between the wild and cultivated sources, including β-sitosterol, quercetin derivatives as well as many newly discovered potential active components, such as trigonelline, arctiin and loganic acid. The results confirm that there is a rich diversity of metabolites in the wild and cultivated YCH, and provide a useful theoretical guidance for the evaluation of quality in the production of YCH.

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Plant metabolomics: a new strategy and tool for quality evaluation of Chinese medicinal materials

Xiao

Liu

et al. 2022

Chin Med

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The present quality control method of Chinese medicinal materials (CMM) has obvious deficiency, which cannot be compatible with the multi-target and multi-component characteristics and production process of CMM. Plant metabolomics with a huge impetus to comprehensively characterize the metabolites and clarify the complexity and integrity of CMM, has been widely used in the research of CMM. This article comprehensively reviewed the application of plant metabolomics in the quality control of CMM. It introduced the concept, technique, and application examples, discussed the prospects, limitations, improvements of plant metabolomics. MS and NMR, as important techniques for plant metabolomics, are mainly highlighted in the case references. The purpose of this article is to clarify the advantage of plants metabolomics for promoting the optimization of the CMM quality control system and proposing a system approach to realize the overall quality control of CMM based on plant metabolomics combined with multidisciplinary method.

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Metabolomics research of medicinal plants

Cited by 3 publications

References 0 publications

Expression and Functional Study of BcWRKY1 in Baphicacanthus cusia (Nees) Bremek

Expression and Functional Study of BcWRKY1 in Baphicacanthus cusia (Nees) Bremek

Metabolomic Analysis Reveals the Metabolic Diversity of Wild and Cultivated Stellaria Radix (Stellaria dichotoma L. var. lanceolata Bge.)

Plant metabolomics: a new strategy and tool for quality evaluation of Chinese medicinal materials

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