Polyphenolic compounds from <i>Malus hupehensis</i> and their free radical scavenging effects

Hu, Qingwen; Chen, Yingying; Jiao, Qiyang; Khan, Afsar; Shan, Jimiao; Cao, Gui-Dong; Li, Feng; Zhang, Chao; Lou, Hongxiang

doi:10.1080/14786419.2017.1367784

Cited by 13 publications

(11 citation statements)

References 27 publications

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“…Authentic standards were chosen based on the LC-MC and NMR spectroscopic results from Malus spp. and M. hupehensis (Li et al 2007;Wang et al 2013;Hu et al 2017), including cyanidin-3-galactoside, cyanidin-3-rutinoside, cyanidin-3-arabinoside, cyanidin-3-glucoside, catechin, chlorogenic acid, ferulic acid, caffeic acid, epicatechin, proanthocyanidin B1, proanthocyanidin B2, rutin, hyperoside, dihydroquercetin, phlorizin and quercetin from Shanghai Yuanye Biological Company.…”

Section: Plant Materials and Phenotypic Characterization -mentioning

confidence: 99%

Analysis of flavonoids and anthocyanin biosynthesis-related genes expression reveals the mechanism of petal color fading of Malus hupehensis (Rosaceae)

et al. 2020

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Malus hupehensis (Pamp.) Rehder is an indigenous wild crabapple tree from China that has received increased attention, due to its significant ornamental and economical values. The flowers change from red to white during development, a phenomenon that is prevalent within Malus genus. However, there are no reports on the phytochemical aspects of M. hupehensis flower. Here, we investigated the mechanism of color fading through biochemical and molecular analysis of M. hupehensis petals during development. Sixteen flavonoids were detected, and cyanidin-3-galactoside was the major anthocyanin in petals. The levels of most compounds initially increased and then decreased during flower development. The changing profile of total anthocyanins was consistent with the changes in floral color. Analysis of anthocyanin biosynthesis-related genes showed that the expression levels of PAL, CHS, CHI, DFR, FLS, ANS, UFGT, MYB10 and MYB12 were significantly higher at early stages than at later stages, similarly to the change in cyanidin-3-galactoside contents. MYB10 and MYB12 significantly positively correlated with CHS, CHI and DFR. There was a particularly strong positive correlation between MYB10 and ANS, and MYB12 was strongly positively correlated with LAR. Thus, MYB10 appeared to regulate mainly anthocyanin biosynthesis, and MYB12 could influence the expression of multiple structural genes in both the anthocyanin and flavanol pathways. By contrast, MYB6, bHLH33 and WD40 were significantly and positively correlated with one another but negatively correlated with the levels of many compounds and most structural genes, indicating that these three transcription factors could regulate together anthocyanin biosynthesis. Our results revealed the information about the temporal and spatial regulation of anthocyanin biosynthesis in M. hupehensis and provide the diverse flower color intensities and patterning in Chinese crabapple.

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Section: Plant Materials and Phenotypic Characterization -mentioning

confidence: 99%

Analysis of flavonoids and anthocyanin biosynthesis-related genes expression reveals the mechanism of petal color fading of Malus hupehensis (Rosaceae)

et al. 2020

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“…The compound varieties were determined by comparing their retention times and UV spectral data with those of the known standards according to reported LC-MC and NMR spectroscopic results of Malus spp. (Li et al, 2007;Hu et al, 2017). The concentration of each compound was calculated using corresponding standard calibration curves with three biological replicates.…”

Section: Hplc-diode Array Detector Analysismentioning

confidence: 99%

How the Color Fades From Malus halliana Flowers: Transcriptome Sequencing and DNA Methylation Analysis

et al. 2020

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The flower color of many horticultural plants fades from red to white during the development stages, affecting ornamental value. We selected Malus halliana, a popular ornamental species, and analyzed the mechanisms of flower color fading using RNA sequencing. Forty-seven genes related to anthocyanin biosynthesis and two genes related to anthocyanin transport were identified; the expression of most of these genes declined dramatically with flower color fading, consistent with the change in the anthocyanin content. A number of transcription factors that might participate in anthocyanin biosynthesis were selected and analyzed. A phylogenetic tree was used to identify the key transcription factor. Using this approach, we identified MhMYB10 as directly regulating anthocyanin biosynthesis. MhMYB10 expression was strongly downregulated during flower development and was significantly positively related to the expression of anthocyanin biosynthetic genes and anthocyanin content in diverse varieties of Malus. To analyze the methylation level during flower development, the MhMYB10 promoter sequence was divided into 12 regions. The methylation levels of the R2 and R8 increased significantly as flower color faded and were inversely related to MhMYB10 expression and anthocyanin content. Therefore, we deduce that the increasing methylation activities of these two regions repressed MhMYB10 expression.

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“…Malus hupehensis (Pamp.) Rehder (M. hupehensis), an edible and medicinal plant, is known as the most important species for ornamental value in the genus Malus, and extensively distributed in southern China [1,2]. As an antipyretic drink with time honored history, a pot of tea by using three pieces of leaves is very popular in Hubei province of China [3,4].…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from Malus hupehensis

Xue

Xiao

et al. 2021

Molecules

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Malus hupehensis (M. hupehensis), an edible and medicinal plant with significant antioxidant and hypoglycemic activity, has been applied to new resource foods. However, the structural characterization and biological effects of its polysaccharides (MHP) are less known. The optimum extraction parameters to achieve the highest extraction efficiency (47.63%), the yield (1.68%) and purity of MHP (89.6%) by ultrasonic-assisted aqueous two-phase system (ATPS) were obtained under the liquid-to-solid ratio of 23 g/mL, ultrasonic power of 65 W, and ultrasonic time of 33 min. According to the analysis results, MHP was composed of Man, GlcA, Rha, GalA, Glc, Gal, Xyl, Ara, and Fuc, in which Ara and Gal were the main components, and the content of GlcA was the lowest. In in vitro activity analysis, MHP showed a significant antioxidant capacity, and an inhibition activity of α-glucosidase and the advanced glycation end products (AGEs) formation in the BSA/Glc reaction model. MHP interacted with α-glucosidase and changed the internal microenvironment of the enzyme, and inhibited the AGEs formation, which provides more evidence for the antihyperglycemic mechanism of MHP. The results suggest that ATPS is an efficient and environmentally friendly solvent system, and M. hupehensis has broad application prospects in functional foods, healthcare products, and pharmaceuticals.

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Polyphenolic compounds from Malus hupehensis and their free radical scavenging effects

Cited by 13 publications

References 27 publications

Analysis of flavonoids and anthocyanin biosynthesis-related genes expression reveals the mechanism of petal color fading of Malus hupehensis (Rosaceae)

Analysis of flavonoids and anthocyanin biosynthesis-related genes expression reveals the mechanism of petal color fading of Malus hupehensis (Rosaceae)

How the Color Fades From Malus halliana Flowers: Transcriptome Sequencing and DNA Methylation Analysis

Ultrasonic-Assisted Aqueous Two-Phase Extraction and Properties of Water-Soluble Polysaccharides from Malus hupehensis

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