Determination of heavy metal toxicity by using a micro-droplet hydrodynamic voltammetry for microalgal bioassay based on alkaline phosphatase

Islam, Md. Saiful; Sazawa, Kazuto; Hata, Noritake; Sugawara, Kazuharu; Kuramitz, Hideki

doi:10.1016/j.chemosphere.2017.09.008

Cited by 15 publications

(7 citation statements)

References 56 publications

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“…The role of rotation rate and substrate concentration on detection of enzyme activity by using RDE has been considered. In our previous study, we have investigated the impacts of rotation on detection of enzyme activity by using RDE [34]. The hydrodynamic chronoamperograms were recorded Fig.…”

Section: Optimization Of Electrochemical Parametersmentioning

confidence: 99%

“…A sensitive determination for PP2A activity can be performed from the oxidation current of PAP by means of hydrodynamic chronoamperometry. The electrochemical behaviors of PAP and PAPP were investigated by hydrodynamic linear sweep voltammetry using RDE according to our previous research work [34]. The study was conducted to obtain the best applied potential for PAP detection where the oxidation of PAP should not be influenced from the effect of PAPP oxidation.…”

Section: Pap Detection By Micro-droplet Hydrodynamic Voltammetrymentioning

confidence: 99%

“…The major advantage of this technique is the decrease in dilution of the product of the enzymatic reaction, which results in high sensitivity and rapid detection for minimizing the nonenzymatic hydrolysis of substrate and product. Our research group previously established micro-droplet hydrodynamic voltammetry for a rapid and simple method for evaluating enzyme activity and genotoxicity [28,[32][33][34].…”

Section: Introductionmentioning

confidence: 99%

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Micro-droplet Hydrodynamic Voltammetry for the Determination of Microcystin-LR Based on Protein Phosphatase

Islam

Sazawa

Sugawara

et al. 2019

J. of Wat. & Envir. Tech.

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Microcystin-LR (MC-LR) is one of the most frequent and most lethal toxicants and a vital environmental pollutant due to its toxicity and persistence. It is hepatotoxins and have been shown to be potent tumor-promoters which pose a serious threat to human health in the form of chronic exposure through drinking water. In this study, a novel electrochemical method for the determination of MC-LR has been developed using protein phosphatase 2A (PP2A) enzyme inhibition. The rapid and simple determination of PP2A activity was accomplished by means of hydrodynamic voltammetry in a 50 µL micro-droplet with a rotating disk electrode (RDE). The chronoamperometric response were obtained from the electrochemical oxidation of p-aminophenol (PAP) following the enzymatic conversion of p-aminophenyl phosphate (PAPP) as a substrate. PP2A activity estimated by using PAPP substrate is affected by MC-LR, and this phenomenon decreases the current signal of PAP. The IC 50 value was calculated as 0.08 µg/L which is well below the World Health Organization (WHO) provisional guideline value for total MC-LR of 1 µg/L in drinking water. The enzymatic reaction using RDE is possible to perform using fewer chemicals in a rapid manner. Therefore, the method can be very attractive for MC contaminated drinking water routine assessment.

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Section: Optimization Of Electrochemical Parametersmentioning

confidence: 99%

Section: Pap Detection By Micro-droplet Hydrodynamic Voltammetrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Micro-droplet Hydrodynamic Voltammetry for the Determination of Microcystin-LR Based on Protein Phosphatase

Islam

Sazawa

Sugawara

et al. 2019

J. of Wat. & Envir. Tech.

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“…Another notable benefit associated with the use of this system is its rapid response due to obtain fast equilibrium. Various reports exist on the use of this detection technique, for example in the magnetic microbead‐based assay for ovalbumin , in the soil enzyme assay , in the gene toxicity assay , and in microalgal bioassay .…”

Section: Resultsmentioning

confidence: 99%

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

et al. 2018

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A rapid and simple electrochemical method was utilized for the determination of the presence of classic fungal metabolite ochratoxin A (OTA) based on differential pulse adsorptive voltammetry, using the carbon nanotube paste (CNTP) electrode. OTA showed a clear oxidation peak, and the CNTP electrode exhibited a more sensitive response to OTA oxidation than the glassy carbon, the plastic formed carbon, and the carbon paste electrodes. The addition of long‐chain cationic surfactants, such as benzethonium chloride, cetyltrimethylammonium bromide, and benzyldimethyltetradecylammonium chloride (zephiramine), enhanced the electrochemical response of OTA. Under optimized experimental conditions, the calibration plots are linear in the 5×10−6−5×10−5 M OTA concentration range, and the limit of detection is 1.1×10−6 M. In order to improve the selectivity for OTA determination, we attempted to combine this adsorptive stripping voltammetric technique with “sequestration electrochemistry,” based on the specific interaction occurring between bovine serum albumin (BSA) and OTA. The peak current of OTA oxidation decreased substantially as a result of OTA‐BSA binding. Furthermore, it was demonstrated that the application of this proposed method of hydrodynamic adsorptive voltammetry, using a micro‐droplet and a rotating disk electrode, improves the approach's sensitivity and reduces the time and sample volume required for the analysis.

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“…Heavy metal pollution is largely due to human activities such as purification of metals, including mining, smelting, ore refinement, preparation of nuclear fuels and other nuclear processing activities, industrial manufacture of batteries, electroplating, paints, pesticides, metal alloys, and many other products and agricultural practices. Heavy metal-rich effluents from these industries can contaminate water sources by adding runoff water to the downstream waterways by rain or by intentional discharge of wastewater by humans (Turdean 2011;Dey 2012;Islam et al 2017). Heavy metal pollution of aquatic ecosystems including oceans, rivers, lakes and reservoirs is now a worldwide severe problem.…”

Section: Introductionmentioning

confidence: 99%

Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems

Rathnayake

Munagamage

Pathirathne

et al. 2021

Water Science and Technology

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Bioavailable content of metals in aquatic systems has become critical in assessing the toxic effect of metals accumulating in the environment. Considering the need for rapid measurements, an optical microalgal-cyanobacterial array biosensor was developed using two strains of microalgae, Mesotaenium sp. and a strain of cyanobacteria Synechococcus sp. to detect Cd2+, Cr6+ and Zn2+ in aquatic systems. Microalgal and cyanobacterial cells were immobilized in a 96-well microplate using sol-gel method using silica. Optimum operational conditions for the biosensor array such as exposure time, storage stability, pH, and multiple metal effect were tested. A 10 min exposure time yielded optimum fluorescence values. Metal toxicity increased with decreasing pH, resulting in low relative fluorescence (%) and decreased with increasing pH, resulting in higher relative fluorescence (%). The optimum storage time for biosensor strains were 4 weeks for microalgal cultures and 8 weeks for cyanobacterial culture, at 4 °C storage temperature. The metal mixtures showed less effect on the inhibition of relative fluorescence (%) of microalgal/cyanobacterial cultures, displaying an antagonistic behavior among the metals tested. As a single unit, this photosynthetic array biosensor will be a valuable tool in detecting multi-metals in aquatic systems.

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Determination of heavy metal toxicity by using a micro-droplet hydrodynamic voltammetry for microalgal bioassay based on alkaline phosphatase

Cited by 15 publications

References 56 publications

Micro-droplet Hydrodynamic Voltammetry for the Determination of Microcystin-LR Based on Protein Phosphatase

Micro-droplet Hydrodynamic Voltammetry for the Determination of Microcystin-LR Based on Protein Phosphatase

Adsorptive Voltammetry for the Determination of Ochratoxin A Using Enrichment Effect by Cationic Surfactants

Whole cell microalgal-cyanobacterial array biosensor for monitoring Cd, Cr and Zn in aquatic systems

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