No abstract

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No abstract

Bioavailable content of metals in aquatic systems has become critical in assessing the toxic effect of metals accumulating in the environment. Considering the need for rapid measurements, an optical microalgal-cyanobacterial array biosensor was developed using two strains of microalgae, Mesotaenium sp. and a strain of cyanobacteria Synechococcus sp. to detect Cd2+, Cr6+ and Zn2+ in aquatic systems. Microalgal and cyanobacterial cells were immobilized in a 96-well microplate using sol-gel method using silica. Optimum operational conditions for the biosensor array such as exposure time, storage stability, pH, and multiple metal effect were tested. A 10 min exposure time yielded optimum fluorescence values. Metal toxicity increased with decreasing pH, resulting in low relative fluorescence (%) and decreased with increasing pH, resulting in higher relative fluorescence (%). The optimum storage time for biosensor strains were 4 weeks for microalgal cultures and 8 weeks for cyanobacterial culture, at 4 °C storage temperature. The metal mixtures showed less effect on the inhibition of relative fluorescence (%) of microalgal/cyanobacterial cultures, displaying an antagonistic behavior among the metals tested. As a single unit, this photosynthetic array biosensor will be a valuable tool in detecting multi-metals in aquatic systems.