“…Further validation analysis revealed that these two compounds could inhibit liver fibrosis by detecting fibrotic gene expression and EMT marker proteins, such as hyaluronic acid, laminin, hydroxyproline, type-I collagen, PAI-1, fibronectin, E -cadherin, vimentin, Snail1, Snail2, and Twist. In addition to the above-mentioned effector molecules, the typical Nrf2/AMPK/mTOR/p-S6K, AMPK/SREBP-1c/FAS, and AMPK/ACC/CPT1 signaling pathways, participating in the regulation of oxidative stress, inflammation, energy metabolism, TGFβ/Smad or non-Smad/ERK/AKT signaling pathway, and Shh signaling pathway, participating in liver fibrosis, were regulated during geniposide and genipin intervention. − Furthermore, genipin was proven to regulate small RNAs and pyroptosis. Genipin specifically increased the expression levels of miR-142a-5p, which bound to the 3′ untranslated region of SREBP-1c to inhibit lipogenesis in HFD-induced male C57BL/6J mice .…”